Isolation, Characterization, and Mapping of Temperature-Sensitive Mutations in the Genes Essential for Lysogenic and Lytic Growth of the Mycobacteriophage L1

Chaudhuri, Biswendu; Sau, Subrata; Datta, Hirock J.; Mandal, Nripendranath

doi:10.1006/viro.1993.1246

Cited by 25 publications

(69 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…M. smegmatis mc 2 6 and LR222, obtained from B. Bloom (Albert Einstein College of Medicine, Bronx, NY, USA) and Anil Tyagi (University of Delhi, South Campus, Delhi, India) respectively, were grown in a Middlebrook 7H9 medium. The composition of the 7H9 medium was given in Chaudhuri et al (1993). The L1cI − and L1cIts391 mycobacteriophage strains were described in Chaudhuri et al (1993).…”

Section: Methodsmentioning

confidence: 99%

“…The composition of the 7H9 medium was given in Chaudhuri et al (1993). The L1cI − and L1cIts391 mycobacteriophage strains were described in Chaudhuri et al (1993). The L1cIts391 lysogen of LR222 was purified from the turbid growth at the center of the plaque that formed at 32 o C (Chaudhuri et al, 1993).…”

Section: Methodsmentioning

confidence: 99%

“…The L1cI − and L1cIts391 mycobacteriophage strains were described in Chaudhuri et al (1993). The L1cIts391 lysogen of LR222 was purified from the turbid growth at the center of the plaque that formed at 32 o C (Chaudhuri et al, 1993). The E. coli promoter-probe vector pTAC3734 was obtained from Dr. T. Atlung (Technical University of Denmark, Denmark), and the E. coli-M. smegmatis shuttle vector pSD5S30 from Dr. Anil Tyagi.…”

Section: Methodsmentioning

confidence: 99%

“…were performed according to the methods described by Sambrook et al, (1989). Mycobacteriophage L1 DNA was isolated as described by Chaudhuri et al, (1993). The isolation of the plasmid DNA from M. smegmatis and the electrotransformation of LR222 were done according to the standard methods of Das Gupta et al, (1993).…”

Section: Dna Isolations and Manipulationsmentioning

confidence: 99%

“…By genetic analyses, the gene coding for the L1 repressor and 28 other genes that are involved in the lytic development of L1 have been mapped. Some were also to an extent characterized (Chaudhuri et al, 1993). Of the 28 genes, the G27 gene was shown to be an early positive regulator that controls the expression of both the delayed early and late genes at the transcriptional level (Datta and Mandal, 1998).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Cloning and Characterization of the Promoters of Temperate Mycobacteriophage L1

Chattopadhyay¹,

Sau²,

Mandal³

2003

BMB Reports

Self Cite

View full text Add to dashboard Cite

Four putative promoters of the temperate mycobacteriophage L1 were cloned by detecting the β-galactosidase reporter expression in E. coli transformants that carried L1 specific operon-fusion library. All of the four L1 promoters were also found to express differentially in the homologous environment of mycobacteria. Of the four promoters, two were suggested to be the putative early promoters of L1 since they express within 0 to 10 min of the initiation of the lytic growth of L1. One of the putative early promoters showed a relatively better and almost identical activity in both E. coli and M. smegmatis. By a sequence analysis, we suggest that the L1 insert that contained the stronger early promoter possibly carries two convergent E. coli σ 70 -like L1 promoters, which are separated from each other by about 300 nucleotides. One of them is the early promoter of L1 as it showed a 100% similarity with the early P left promoter of the homoimmune phage L5. The second promoter, designated P4, was suggested for its appreciable level of reporter activity in the absence of the −10 element of the P left equivalent of L1. By analyzing most of the best characterized mycobacteriophages-specific promoters, including the L1 promoter P4, we suggest that both the − 10 and −35 hexamers of the mycobacteriophage promoters are highly conserved and almost similar to the consensus − 10 and −35 hexamers of the E. coli σ 70 promoters.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Dna Isolations and Manipulationsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Cloning and Characterization of the Promoters of Temperate Mycobacteriophage L1

Chattopadhyay¹,

Sau²,

Mandal³

2003

BMB Reports

Self Cite

View full text Add to dashboard Cite

show abstract

Dynamics of Mycobacteriophage—Mycobacterial Host Interaction

Ghosh

Phukan

Johari

et al. 2020

Methods in Molecular Biology

View full text Add to dashboard Cite

A high yielding mutant of mycobacteriophage L1 and its application as a diagnostic tool

Chatterjee

2000

FEMS Microbiology Letters

View full text Add to dashboard Cite

L1 is a lysogenic phage of mycobacteria, which along with L5 and D29 constitute a closely linked family of homoimmune mycobacteriophages. These phages can be potentially used for genetic engineering of mycobacteria and diagnosis of mycobacterial infection. The effectiveness of such phage based systems depends on the efficiency with which they infect and grow within target cells. While working with phage L1c1ts which is a temperature sensitive mutant of phage L1, we observed that high yielding phage stocks were generated by repeated passage through the host, Mycobacterium smegmatis. A plaque purified mutant L1-P2, obtained from one such high yielding stock, when analyzed further was found to infect host cells with increased efficiency. The DNA obtained from L1-P2 was examined by restriction digestion, and it was observed that spontaneous loss of DNA fragment from the right arm, which encodes early regulatory factors, had occurred. It has been further demonstrated that the high yielding property of the mutant phage could be utilized to increase the sensitivity of mycobacteriophage-based detection systems. ß

show abstract

Isolation, Characterization, and Mapping of Temperature-Sensitive Mutations in the Genes Essential for Lysogenic and Lytic Growth of the Mycobacteriophage L1

Cited by 25 publications

References 0 publications

Cloning and Characterization of the Promoters of Temperate Mycobacteriophage L1

Cloning and Characterization of the Promoters of Temperate Mycobacteriophage L1

Dynamics of Mycobacteriophage—Mycobacterial Host Interaction

A high yielding mutant of mycobacteriophage L1 and its application as a diagnostic tool

Contact Info

Product

Resources

About