Isolation, Characterization and MicroRNA-based Genetic Modification of Human Dental Follicle Stem Cells

Müller, Paula; Ekat, Katharina; Brosemann, Anne; Köntges, Anne; Dávid, Róbert; Lang, Hermann

doi:10.3791/58089

Cited by 5 publications

(4 citation statements)

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“…Dental follicle stem cells can be isolated by an enzymatic digestion with collagenase I and trypsin after separating the dental follicle tissue from the dental crown. Afterwards, the cells can be seeded, and only the DFSCs will adhere to plastic cell culture dishes [38]. The isolation of SCAP from the apical papilla can be done accordingly [27].…”

Section: Isolation and Characterization Of Stem Cells From Dental Tissuesmentioning

confidence: 99%

Sinking Our Teeth in Getting Dental Stem Cells to Clinics for Bone Regeneration

Shoushrah

Transfeld

Tonk

et al. 2021

IJMS

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Dental stem cells have been isolated from the medical waste of various dental tissues. They have been characterized by numerous markers, which are evaluated herein and differentiated into multiple cell types. They can also be used to generate cell lines and iPSCs for long-term in vitro research. Methods for utilizing these stem cells including cellular systems such as organoids or cell sheets, cell-free systems such as exosomes, and scaffold-based approaches with and without drug release concepts are reported in this review and presented with new pictures for clarification. These in vitro applications can be deployed in disease modeling and subsequent pharmaceutical research and also pave the way for tissue regeneration. The main focus herein is on the potential of dental stem cells for hard tissue regeneration, especially bone, by evaluating their potential for osteogenesis and angiogenesis, and the regulation of these two processes by growth factors and environmental stimulators. Current in vitro and in vivo publications show numerous benefits of using dental stem cells for research purposes and hard tissue regeneration. However, only a few clinical trials currently exist. The goal of this review is to pinpoint this imbalance and encourage scientists to pick up this research and proceed one step further to translation.

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Section: Isolation and Characterization Of Stem Cells From Dental Tissuesmentioning

confidence: 99%

Sinking Our Teeth in Getting Dental Stem Cells to Clinics for Bone Regeneration

Shoushrah

Transfeld

Tonk

et al. 2021

IJMS

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“…The DFSCs are able to differentiate into neural-like cells, when cultured in a neural inductor medium [113]. These cells were proven to possess characteristics of mesenchymal stem cells (MSCs) including self-renewing capacity, plastic adherence, expression of specific surface markers: CD73, CD90, CD105 [114]. hDFSCs have been shown to secrete a significant number of essential factors, such as matrix metalloproteinases (MMPs), insulin-like growth factor (IGF), vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), and hepatocyte growth factor (HGF), factors with a key role in angiogenesis, immunomodulation, and regenerative process [114].…”

Section: Dental Follicle Stem Cells Dfscsmentioning

confidence: 99%

Angiogenesis in Regenerative Dentistry: Are We Far Enough for Therapy?

Baru

Nuțu

Braicu

et al. 2021

IJMS

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Angiogenesis is a broad spread term of high interest in regenerative medicine and tissue engineering including the dental field. In the last two decades, researchers worldwide struggled to find the best ways to accelerate healing, stimulate soft, and hard tissue remodeling. Stem cells, growth factors, pathways, signals, receptors, genetics are just a few words that describe this area in medicine. Dental implants, bone and soft tissue regeneration using autologous grafts, or xenografts, allografts, their integration and acceptance rely on their material properties. However, the host response, through its vascularization, plays a significant role. The present paper aims to analyze and organize the latest information about the available dental stem cells, the types of growth factors with pro-angiogenic effect and the possible therapeutic effect of enhanced angiogenesis in regenerative dentistry.

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“…Briefly, DF tissue is dissected from the upside of the dental crown and incubated with a dissociative solution containing collagenase and trypsin. Followed by cell seeding, the subset population of DFSCs would adhere to the plastic dish surface, while non-adherent cells are removed simply by the media change [73]. DFSCs express CD9, CD10, CD13, CD29, CD44, CD53, CD59, CD73, CD106, CD166, and CD271, but not hematopoietic markers including CD 117, CD31, CD34, CD45, and CD133 [14].…”

Section: Dental Follicle Stem Cells (Dfscs)mentioning

confidence: 99%

Therapeutic Functions of Stem Cells from Oral Cavity: An Update

Yang

Shin

Seo

et al. 2020

IJMS

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Adult stem cells have been developed as therapeutics for tissue regeneration and immune regulation due to their self-renewing, differentiating, and paracrine functions. Recently, a variety of adult stem cells from the oral cavity have been discovered, and these dental stem cells mostly exhibit the characteristics of mesenchymal stem cells (MSCs). Dental MSCs can be applied for the replacement of dental and oral tissues against various tissue-damaging conditions including dental caries, periodontitis, and oral cancers, as well as for systemic regulation of excessive inflammation in immune disorders, such as autoimmune diseases and hypersensitivity. Therefore, in this review, we summarized and updated the types of dental stem cells and their functions to exert therapeutic efficacy against diseases.

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Isolation, Characterization and MicroRNA-based Genetic Modification of Human Dental Follicle Stem Cells

Cited by 5 publications

References 0 publications

Sinking Our Teeth in Getting Dental Stem Cells to Clinics for Bone Regeneration

Sinking Our Teeth in Getting Dental Stem Cells to Clinics for Bone Regeneration

Angiogenesis in Regenerative Dentistry: Are We Far Enough for Therapy?

Therapeutic Functions of Stem Cells from Oral Cavity: An Update

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