Isolation, characterization and optimization of EPSs produced by a cold-adapted<i>Marinobacter</i>isolate from Antarctic seawater

Caruso, Consolazione; Rizzo, Carmen; Mangano, Santina; Poli, Annarita; Donato, Paola Di; Nicolaus, Barbara; Finore, Ilaria; Marco, G. Di; Michaud, Luigi; Giudice, Angelina Lo

doi:10.1017/s0954102018000482

Cited by 30 publications

(21 citation statements)

References 39 publications

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“…grown on alkane formed cell aggregates supported by enhanced EPS production (Sabirova et al, 2011). Analysis of EPS produced by Marinobacter isolates revealed high levels of carbohydrates with a broad range of structural monosaccharides (Caruso et al, 2019), including xylose (Bhaskar et al, 2005). Members of the genus Colwellia isolated from cold waters and sediments produced cryoprotectant EPS (Marx et al, 2009) as well as EPS enriched in deoxysugarcontaining polysaccharides (Casillo et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Polysaccharide hydrolysis in the presence of oil and dispersants: Insights into potential degradation pathways of exopolymeric substances (EPS) from oil-degrading bacteria

Ziervogel

Joye

Kleindienst

et al. 2019

Elementa: Science of the Anthropocene

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Oceanic oil-degrading bacteria produce copious amounts of exopolymeric substances (EPS) that facilitate their access to oil. The fate of EPS in the water column is in part determined by activities of heterotrophic microbes capable of utilizing EPS compounds as carbon and energy sources. To evaluate the potential of natural microbial communities to degrade EPS produced during oil degradation, we measured potential hydrolysis rates of six structurally distinct polysaccharides in two roller bottle experiments, using water from a natural oil seep in the northern Gulf of Mexico. The suite of polysaccharides used to measure the initial step in carbon degradation is indicative of polymers within microbial EPS. The treatments included (i) unamended surface or deep waters (whole water), and water amended with (ii) a water-accommodated fraction of oil (WAF), (iii) oil dispersant Corexit 9500, and (iv) WAF chemically-enhanced with Corexit (CEWAF). The oil and Corexit treatments were employed to simulate conditions during the Deepwater Horizon oil spill. Polysaccharide hydrolysis rates in the surface-water treatments were lowest in the WAF treatment, despite elevated levels of EPS in the form of transparent exopolymer particles (TEP). In contrast, the three deep-water treatments (WAF, Corexit, CEWAF) showed enhanced hydrolysis rates and TEP levels (WAF) compared to the whole water. We also observed variations in the spectrum of polysaccharide-hydrolyzing enzyme activities among the treatments. These substrate specificities were likely driven by activities of oil-degrading bacteria, shaping the pool of EPS and TEP as well as degradation products of hydrocarbons and Corexit compounds. A model calculation of potential turnover rates of organic carbon within the TEP pool suggests extended residence times of TEP in oil-contaminated waters, making them prone to serve as the sticky matrix for oily aggregates known as marine oil snow.

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Section: Discussionmentioning

confidence: 99%

Polysaccharide hydrolysis in the presence of oil and dispersants: Insights into potential degradation pathways of exopolymeric substances (EPS) from oil-degrading bacteria

Ziervogel

Joye

Kleindienst

et al. 2019

Elementa: Science of the Anthropocene

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“…Although Marinobacter can use hydrocarbons as carbon source, various studies demonstrated that it can also grow and produce EPS on other carbon sources such as glucose. Marinobacter species have been shown to produce exopolysaccharide polymers with excellent emulsifying activity against hydrocarbons that were superior to commercial synthetic surfactants like Tween 80 (Caruso et al, 2019 ). Marinobacter sp.…”

Section: Marine Biosurfactant-producing Bacteriamentioning

confidence: 99%

“…The highest yield was produced when the culture was grown at 15°C and in the presence of 2% glucose. However, the strain was able to synthesize EPS, even at 4°C, albeit in lower quantities, suggesting that the EPS might have cryoprotective functions (Caruso et al, 2019 ). In addition, Marinobacter sp.…”

Section: Marine Biosurfactant-producing Bacteriamentioning

confidence: 99%

Biosurfactants and Their Applications in the Oil and Gas Industry: Current State of Knowledge and Future Perspectives

Nikolova

Gutiérrez

2021

Front. Bioeng. Biotechnol.

114

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Surfactants are a group of amphiphilic chemical compounds (i.e., having both hydrophobic and hydrophilic domains) that form an indispensable component in almost every sector of modern industry. Their significance is evidenced from the enormous volumes that are used and wide diversity of applications they are used in, ranging from food and beverage, agriculture, public health, healthcare/medicine, textiles, and bioremediation. A major drive in recent decades has been toward the discovery of surfactants from biological/natural sources—namely bio-surfactants—as most surfactants that are used today for industrial applications are synthetically-manufactured via organo-chemical synthesis using petrochemicals as precursors. This is problematic, not only because they are derived from non-renewable resources, but also because of their environmental incompatibility and potential toxicological effects to humans and other organisms. This is timely as one of today's key challenges is to reduce our reliance on fossil fuels (oil, coal, gas) and to move toward using renewable and sustainable sources. Considering the enormous genetic diversity that microorganisms possess, they offer considerable promise in producing novel types of biosurfactants for replacing those that are produced from organo-chemical synthesis, and the marine environment offers enormous potential in this respect. In this review, we begin with an overview of the different types of microbial-produced biosurfactants and their applications. The remainder of this review discusses the current state of knowledge and trends in the usage of biosurfactants by the Oil and Gas industry for enhancing oil recovery from exhausted oil fields and as dispersants for combatting oil spills.

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“…A plethora of ecological roles are played by EPSs in cold environments. Beside acting as osmoprotectants, EPSs produced by cold-adapted bacteria have been reported as ROS scavengers and cryoprotectants [ 147 , 148 , 149 , 150 ]. For example, high concentrations of EPSs are produced at low and sub-freezing temperatures by Pseudoalteromonas , Winogradskyella and Marinobacter isolates, probably acting as a diffusion barrier to solutes and a physical-like barrier to ice formation [ 148 , 149 ].…”

Section: Bacteriamentioning

confidence: 99%

Physiological and Molecular Responses to Main Environmental Stressors of Microalgae and Bacteria in Polar Marine Environments

et al. 2020

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The Arctic and Antarctic regions constitute 14% of the total biosphere. Although they differ in their physiographic characteristics, both are strongly affected by snow and ice cover changes, extreme photoperiods and low temperatures, and are still largely unexplored compared to more accessible sites. This review focuses on microalgae and bacteria from polar marine environments and, in particular, on their physiological and molecular responses to harsh environmental conditions. The data reported in this manuscript show that exposure to cold, increase in CO2 concentration and salinity, high/low light, and/or combination of stressors induce variations in species abundance and distribution for both polar bacteria and microalgae, as well as changes in growth rate and increase in cryoprotective compounds. The use of -omics techniques also allowed to identify specific gene losses and gains which could have contributed to polar environmental adaptation, and metabolic shifts, especially related to lipid metabolism and defence systems, such as the up-regulation of ice binding proteins, chaperones and antioxidant enzymes. However, this review also provides evidence that -omics resources for polar species are still few and several sequences still have unknown functions, highlighting the need to further explore polar environments, the biology and ecology of the inhabiting bacteria and microalgae, and their interactions.

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Isolation, characterization and optimization of EPSs produced by a cold-adaptedMarinobacterisolate from Antarctic seawater

Cited by 30 publications

References 39 publications

Polysaccharide hydrolysis in the presence of oil and dispersants: Insights into potential degradation pathways of exopolymeric substances (EPS) from oil-degrading bacteria

Polysaccharide hydrolysis in the presence of oil and dispersants: Insights into potential degradation pathways of exopolymeric substances (EPS) from oil-degrading bacteria

Biosurfactants and Their Applications in the Oil and Gas Industry: Current State of Knowledge and Future Perspectives

Physiological and Molecular Responses to Main Environmental Stressors of Microalgae and Bacteria in Polar Marine Environments

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