Isolation, characterization, and surface chemistry of a surface-active fraction from dog lung

Frosolono, M.F.; Charms, Bernard L.; Pawłowski, Ryszard; Slivka, Sandra R.

doi:10.1016/s0022-2275(20)42954-2

Cited by 183 publications

(20 citation statements)

References 61 publications

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“…However, the yields of material we obtained (Table 1) are similar to those of other workers (e.g. Abrams, 1966;Frosolono et al, 1970) and are of the same order as those suggested by King & Clements (1972b).…”

Section: Resultssupporting

confidence: 90%

“…in the preparation from sheep lung. Although the overall protein content of the surfactant samples was comparable with that for the most surface-active preparations reported (Dickie et al, 1973;Frosolono et al, 1970;King & Clements, 1972a;Klein & Margolis, 1968), more' individual bands were visible on the polyacrylamide gels than on those of King (1974) for preparations from dog. This most probably reflects differences in lavage and in the-isolation prooedures rather than in species variations.…”

Section: Resultssupporting

confidence: 54%

“…The surfactant lipids from dog are similar in overall composition (Frosolono et al, 1970;King, 1974;King & Clements, 1972b) except that cholesterol represented a higher and triacylglycerol a lower percentage than in the surfactant samples we isolated. Vol.…”

Section: Resultsmentioning

confidence: 64%

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Characterization of pulmonary surfactant from ox, rabbit, rat and sheep

Harwood¹,

Desai²,

Hext³

et al. 1975

Biochemical Journal

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1. Pulmonary surfactants from ox, rabbit, rat and sheep were isolated and analysed. 2. All preparations had a high anenoic phosphatidylcholine content and would produce stable surface tensions of 0.01 Nm-1 or less. 3. Protein content was 8-18% of the dry weights. A number of proteins were observed; their overall composition were high in hydrophobic amino acid residues. 4. Lipid content varied from 79% (ox) to 90% (rabbit) with phosphatidylcholine representing from 58% (sheep) to 83% (rabbit) of the total lipid. The surfactant preparations were rather similar in lipid composition except that sheep surfactant contained about 10% lysophosphatidylcholine. 5. Hexadecanoic acid was the principal fatty acid. It was particularly high in phosphatidylcholine. 6. Phosphatidylglycerol was a minor constituent of all surfactants but phosphatidyldimethylethanolamine was not detected.

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Section: Resultssupporting

confidence: 90%

Section: Resultssupporting

confidence: 54%

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Characterization of pulmonary surfactant from ox, rabbit, rat and sheep

Harwood¹,

Desai²,

Hext³

et al. 1975

Biochemical Journal

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“…These were the fractions that were morphologically pure and contained surface activity. The fraction containing the common myelin figures (F I) was studied because the physical characteristics and chemical composition of this fraction are similar to the surface-active fractions isolated from lung lavage of rabbit (37) and dog (66,67) . Electron micrographs of this fraction showed mostly osmiophilic structures that by morphological criteria could be interpreted as damaged lamellar bodies that had lost their limiting membrane and amorphous material ; on the other hand, protein analyses indicated the presence of protein not Dipalmitoyl lecithin, first isolated from lung by Thannhauser (72) was subsequently identified as one of the main components of surface-active lung extracts (73) .…”

Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of Lamellar Bodies and Tubular Myelin From Rat Lung Homogenates

Gil

Reiss

1973

The Journal of Cell Biology

251

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Three surface-active fractions which differ in their morphology have been isolated from rat lung homogenates by ultracentrifugation in a discontinuous sucrose density gradient . In order of increasing density, the fractions consisted, as shown by electron microscopy, primarily of common myelin figures, lamellar bodies, and tubular myelin figures . The lipid of all three fractions contained approximately 94% polar lipids and 2 % cholesterol . In the case of the common myelin figures and the lamellar bodies, the polar lipids consisted of 73% phosphatidylcholines, 9 % phosphatidylserines and inositols, and 8 % phosphatidylethanolamines. In the case of the tubular myelin figures, the respective percentages were 58, 19, and 5 . Over 90% of the fatty acids of the lecithins of all three fractions were saturated . Electrophoresis of the proteins of the fractions in sodium dodecyl sulfate or Triton X-100 revealed that the lamellar bodies and the tubular myelin figures differed in the mobilities of their proteins . The common myelin figures, however, contained proteins from both of the other fractions. These data indicate that, whereas the lipids of the extracellular, alveolar surfactant(s) originate in the lamellar bodies, the proteins arise from another source . It is further postulated that the tubular myelin figures represent a liquid crystalline state of the alveolar surface-active lipoproteins.

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“…Extensive studies (e.g. by Frosolono et al, 1970;Gil &Reiss, 1973;Rooney et al, 1975) have shown that the composition and properties of lamellar-body material resembles, but is not identical with, surfactant isolated by endobronchial lavage. Phospholipid synthesis has been studied by using lamellar bodies (Spitzer et al, 1975), and it is likely that material from this source forms most of the pulmonary surfactant secreted into the alveoli.…”

mentioning

confidence: 99%

Changes in pulmonary surfactant and phosphatidylcholine metabolism in rats exposed to chrysotile asbestos dust

Tetley¹,

Richards²,

Harwood³

1977

Biochemical Journal

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1. Pulmonary surfactant was isolated from rats that had been exposed to chrysotile asbestos dust for from 3 days to 15 weeks. 2. Asbestos-treated rats showed a progressive increase in amounts of surfactant. After 15 weeks, treated animals contained 4 times as much as non-treated. 3. No significant change was seen in the total protein or total fatty acid composition of surfactant with exposure. 4. The increase in surfactant phosphatidylcholine normally seen on maturation of rat lung was accelerated by exposure of animals to asbestos. 5. An increase in the activity of phosphorylcholine glyceride transferase in lung homogenates and free cell populations was found. 6. Lysosomal phospholipase A was relatively unaffected by dust exposure. 7. It is suggested that the increase in surfactant amounts could be due to an increase in its synthesis without a corresponding alteration in its degradation.

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Isolation, characterization, and surface chemistry of a surface-active fraction from dog lung

Cited by 183 publications

References 61 publications

Characterization of pulmonary surfactant from ox, rabbit, rat and sheep

Characterization of pulmonary surfactant from ox, rabbit, rat and sheep

Isolation and Characterization of Lamellar Bodies and Tubular Myelin From Rat Lung Homogenates

Changes in pulmonary surfactant and phosphatidylcholine metabolism in rats exposed to chrysotile asbestos dust

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