Isolation, characterization, and survival strategies of Thermotoga sp. strain PD524, a hyperthermophile from a hot spring in Northern Thailand

Kanoksilapatham, Wirojne; Keawram, Porranee; Gonzalez, Juan M.; Robb, Frank T.

doi:10.1007/s00792-015-0761-2

Cited by 9 publications

(6 citation statements)

References 35 publications

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“…Other phyla, such as Thermi and Thermotogae were also found abundant at both the sites. Phylum Thermotogae observed as abundant in Tattapani is found associated with hot springs of high temperature and is reported majorly for its high heat tolerance (Chan et al, 2015; Kanoksilapatham et al, 2015). Tepidimonas sp., belonging to phylum Proteobacteria observed as abundant in BAN, CAN, TAT-2, TAT-4, and CAP samples, has been isolated and sequenced from Chhoti Anhoni in an earlier study (Dhakan et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Metagenomic Analysis of Hot Springs in Central India Reveals Hydrocarbon Degrading Thermophiles and Pathways Essential for Survival in Extreme Environments

et al. 2017

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Extreme ecosystems such as hot springs are of great interest as a source of novel extremophilic species, enzymes, metabolic functions for survival and biotechnological products. India harbors hundreds of hot springs, the majority of which are not yet explored and require comprehensive studies to unravel their unknown and untapped phylogenetic and functional diversity. The aim of this study was to perform a large-scale metagenomic analysis of three major hot springs located in central India namely, Badi Anhoni, Chhoti Anhoni, and Tattapani at two geographically distinct regions (Anhoni and Tattapani), to uncover the resident microbial community and their metabolic traits. Samples were collected from seven distinct sites of the three hot spring locations with temperature ranging from 43.5 to 98°C. The 16S rRNA gene amplicon sequencing of V3 hypervariable region and shotgun metagenome sequencing uncovered a unique taxonomic and metabolic diversity of the resident thermophilic microbial community in these hot springs. Genes associated with hydrocarbon degradation pathways, such as benzoate, xylene, toluene, and benzene were observed to be abundant in the Anhoni hot springs (43.5–55°C), dominated by Pseudomonas stutzeri and Acidovorax sp., suggesting the presence of chemoorganotrophic thermophilic community with the ability to utilize complex hydrocarbons as a source of energy. A high abundance of genes belonging to methane metabolism pathway was observed at Chhoti Anhoni hot spring, where methane is reported to constitute >80% of all the emitted gases, which was marked by the high abundance of Methylococcus capsulatus. The Tattapani hot spring, with a high-temperature range (61.5–98°C), displayed a lower microbial diversity and was primarily dominated by a nitrate-reducing archaeal species Pyrobaculum aerophilum. A higher abundance of cell metabolism pathways essential for the microbial survival in extreme conditions was observed at Tattapani. Taken together, the results of this study reveal a novel consortium of microbes, genes, and pathways associated with the hot spring environment.

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Section: Discussionmentioning

confidence: 99%

Metagenomic Analysis of Hot Springs in Central India Reveals Hydrocarbon Degrading Thermophiles and Pathways Essential for Survival in Extreme Environments

et al. 2017

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“…In Bor Khlueng (Thailand), the majority of the detected prokaryotic sequences were unknown (approximately 80%) (Kanokratana et al, 2004). For example, Kanoksilapatham et al (2015) studied a hot spring in northern Thailand where a strain of hyperthermophilic Thermotoga sp. (strain PD524) was isolated.…”

Section: Relative Abundancementioning

confidence: 99%

“…From a hot spring in northern Thailand, the phylum Thermotogae-Thermotoga sp. strain PD524 was isolated (70-85 °C, pH 6.0-8.5, NaCl 0-10 g/L) (Kanoksilapatham et al, 2015). In a hot spring pond in the Krabi province (Thailand), the genus Planosporangium, a novel filamentous bacterial strain, HSS8-18 (T), was isolated from the soil (Thawai et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

The Chemical Characteristic and Microbial Diversity of the Hot Spring at Phusang National Park

Bumrungthai

Duangjit

Somsuwan³

et al. 2019

Environ. Nat. Resour. J.

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The robustness of Phusang's warm waterfall is regionally recognized as the only one with a temperature range of 35-36 °C which makes it an outstanding place to visit in Thailand. Surprisingly, Phusang waterfall originates from the Phusang warm pool (hot spring). However, data about the bacterial community and characteristics of this water are still obscure. Therefore, this study investigated the bacterial community and characteristics of water in the Phusang hot spring. Tests to determine its physical characteristics such as pH, color and turbidity were performed. Trace elements such as sodium, bicarbonates, iron, and fluoride were detected as chemical characteristics. The biological properties were verified by 16S ribosomal RNA sequencing. Illumina metagenomic analysis was directly demonstrated from the water after DNA extraction via a membrane filtration pore of 0.45 µm. The range of pH, color and turbidity of water from the Phusang hot spring was 7.33-7.53, 0.05-0.18 Pt.Co and 9.55-10.91 NTU, respectively. The biological study of microorganisms found less than 300 CFU/mL. Coliform bacteria such as Staphylococcus aureus and other examples such as Aeromonas veronii, Acinetobacter sp. Neisseriaceae bacterium were abundant. Shotgun metagenomic sequencing defined the phylum as Proteobacteria (84%), Bacteroidetes (13%), Cyanobacteria (1%), and unclassified (2%). Moreover, the amount of sodium and strontium detected was 6.00-7.52 and 1.40-1.58 mg/L respectively. These studies show that a high abundance of Proteobacteria were present in samples from this hot spring. Phusang hot spring has been classified as having low mineral content water.

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“… [18] , Thermotoga sp. strain PD524 [19] . Ekundayo and Killham [20] reported the solubilization and accumulation of lead by two strains of Pseudomonas at concentrations of 0.03 and 0.07 mg mL −1 .…”

Section: Introductionmentioning

confidence: 99%

Study on bioremediation of Lead by exopolysaccharide producing metallophilic bacterium isolated from extreme habitat

Kalita

Joshi

2017

Biotechnology Reports

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Isolation, characterization, and survival strategies of Thermotoga sp. strain PD524, a hyperthermophile from a hot spring in Northern Thailand

Cited by 9 publications

References 35 publications

Metagenomic Analysis of Hot Springs in Central India Reveals Hydrocarbon Degrading Thermophiles and Pathways Essential for Survival in Extreme Environments

Metagenomic Analysis of Hot Springs in Central India Reveals Hydrocarbon Degrading Thermophiles and Pathways Essential for Survival in Extreme Environments

The Chemical Characteristic and Microbial Diversity of the Hot Spring at Phusang National Park

Study on bioremediation of Lead by exopolysaccharide producing metallophilic bacterium isolated from extreme habitat

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