Isolation, Colonization, and Chlorpyrifos Degradation Mediation of the Endophytic Bacterium <i>Sphingomonas</i> Strain HJY in Chinese Chives (<i>Allium tuberosum</i>)

Feng, Fayun; Ge, Jing; Li, Yisong; Cheng, Jinjin; Zhong, Jianfeng; Yu, Xiangyang

doi:10.1021/acs.jafc.6b05283

Cited by 49 publications

(26 citation statements)

References 37 publications

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“…An endophytic bacterium Sphingomonas sp. strain HJY isolated from the leaves of Chinese leek ( Allium tuberosum ) showed the potential ability to degrade Chlorpyrifos 14 . The fungal endophyte Clonostachys rosea ICIPE 707 isolated from healthy onion ( Allium cepa ) colonized onion plants and significantly repressed the feeding punctures, oviposition, and numbers of thrips on plants 15 .…”

Section: Introductionmentioning

confidence: 99%

Illumina-based Analysis of Endophytic Bacterial Diversity of four Allium species

Huang

2019

Sci Rep

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Allium species are popular vegetables in China and possess antifungal and antibacterial activities. This study aimed to compare the endophytic bacterial community in the four crucial Allium species in China, Chinese leek (CL), garlic (GA), onion (ON,) and Welsh onion (WO), using sequences of the V3–V4 region of the bacterial 16S rRNA gene. A total of 1,036,637 high-quality sequences and 719 operational taxonomic units (OTUs) were obtained across all libraries. A total of 20 phyla, 50 classes, 80 orders, 134 families, and 234 genera were identified. Among them, 18 OTUs and 19 genera were shared among the four Allium species. Proteobacteria (42.68%) and Bacteroidetes (20.18%) were the dominant phyla in CL, while one unclassified (>70%) was the dominant phyla in the other three Allium species. The alpha-diversity analysis showed the bacterial richness and diversity in CL were significantly higher than those in the other three Allium species. Principal coordinate analysis (PCA) showed endophytic bacterial communities in GA, WO, and ON were more similar than those in CL. Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) analysis revealed endophytic bacteria mostly enriched in Membrane Transport, Amino Acid Metabolism and Carbohydrate Metabolism pathway. 17 of the 23 Kyoto Encyclopedia of Genes and Genomes (KEGG) categories and 159 of the 206 lower-level KEGG pathways in CL were significantly higher than those in the other three Allium species. Pearson’s correlation indicated that KEGG pathways with significant differences among the Allium species were closely related to the bacterial genera with significant differences between the Allium species. The findings of our study provided insight into the complex endophytic microbial communities in Allium species.

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Section: Introductionmentioning

confidence: 99%

Illumina-based Analysis of Endophytic Bacterial Diversity of four Allium species

Huang

2019

Sci Rep

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“…In this context, endophytic bacteria Sphingomonas sp. HJY isolated from Chinese chives were shown to lower the content of CP in host plants and in liquid medium in an in vitro study [65,66]. Likewise, in vitro and in vivo assays corroborated the simultaneous degradation of fluazinam, BHC, PCNB, CP, and DDT into non-toxic alkanes in ginseng roots, stems, and leaves by endophytic Paenibacillus polymyxa [67].…”

Section: Removal Of Agrochemicals-pesticides/herbicides/insecticides/fertilizersmentioning

confidence: 71%

Selection of Endophytic Strains for Enhanced Bacteria-Assisted Phytoremediation of Organic Pollutants Posing a Public Health Hazard

Karaś¹,

Wdowiak-Wróbel²,

Sokołowski³

2021

IJMS

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Anthropogenic activities generate a high quantity of organic pollutants, which have an impact on human health and cause adverse environmental effects. Monitoring of many hazardous contaminations is subject to legal regulations, but some substances such as therapeutic agents, personal care products, hormones, and derivatives of common organic compounds are currently not included in these regulations. Classical methods of removal of organic pollutants involve economically challenging processes. In this regard, remediation with biological agents can be an alternative. For in situ decontamination, the plant-based approach called phytoremediation can be used. However, the main disadvantages of this method are the limited accumulation capacity of plants, sensitivity to the action of high concentrations of hazardous pollutants, and no possibility of using pollutants for growth. To overcome these drawbacks and additionally increase the efficiency of the process, an integrated technology of bacteria-assisted phytoremediation is being used recently. For the system to work, it is necessary to properly select partners, especially endophytes for specific plants, based on the knowledge of their metabolic abilities and plant colonization capacity. The best approach that allows broad recognition of all relationships occurring in a complex community of endophytic bacteria and its variability under the influence of various factors can be obtained using culture-independent techniques. However, for practical application, culture-based techniques have priority.

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“…This species was capable of transforming chlorpyrifos to 3,5,6-trichloro-2-pyridinol (TCP) [18]. Sphingomonas sp utilizes chlorpyrifos as sole source of carbon for growth, by hydrolyzing chlorpyrifos to 3,5,6-trichloro-2-pyridinol (TCP) [11]. Bacillus or paenibacillus groups catalyzed chlorpyrifos through hydrolysis of C-O-P ester bonds by phosphatase or phytase enzymes and released soluble Fig.…”

Section: Discussionmentioning

confidence: 99%

Chlorpyrifos biodegradation in relation to metabolic attributes and 16S rRNA gene phylogeny of bacteria in a tropical vertisol

et al. 2019

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It is predicted that use of insecticide chlorpyrifos will increase in near future due to intensive agricultural practice and climate change. The present study envisages establishing linkages among biodegradation potential, carbon metabolism and enzymatic attributes of bacteria involved in chlorpyrifos biodegradation. Soil samples from an experimental field were incubated with chlorpyrifos (10 µg g −1 soil). Chlorpyrifos biodegradation rate was 0.34 ± 0.04 ug g −1 soil d −1 . Culturable bacteria were isolated and identified by 16S rRNA gene sequencing. DNA sequences were 96-100% similar to the GenBank bacterial DNA sequences. The bacterial community was predominated by Arthrobacter phenanthrenivorans (23%). Followed by Arthrobacter globiformis and Bacillus flexus represented 15% each. Microbacterium paraoxydans, Bacillus megaterium, Bacillus soli strain R, Bacillus drentensis, and Bacillus koreensis represented 8% of total bacteria. The carbon source utilization pattern and enzymatic activities of the bacterial isolates were estimated. The isolates preferred to metabolize trehalose and mannose over other C sources. These bacteria exhibited decarboxylase, oxidase, catalase, cellulose, deaminase and nitrate reductase activities. A PCA analysis based on various metabolic attributes explained 32.49% variance by PC1 and 16.46% variance by PC2. PCA highlights that the bacterial isolates placed distantly from those of simple sugar utilizers, probably use chlorpyrifos as C substrate. Correspondence analysis outlaid decarboxylase, catalase, and oxidase as the key enzymatic activities of the bacterial isolates for chlorpyrifos biodegradation.

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Isolation, Colonization, and Chlorpyrifos Degradation Mediation of the Endophytic Bacterium Sphingomonas Strain HJY in Chinese Chives (Allium tuberosum)

Cited by 49 publications

References 37 publications

Illumina-based Analysis of Endophytic Bacterial Diversity of four Allium species

Illumina-based Analysis of Endophytic Bacterial Diversity of four Allium species

Selection of Endophytic Strains for Enhanced Bacteria-Assisted Phytoremediation of Organic Pollutants Posing a Public Health Hazard

Chlorpyrifos biodegradation in relation to metabolic attributes and 16S rRNA gene phylogeny of bacteria in a tropical vertisol

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