Isolation, Detection and Characterization of Aerobic Bacteria from Honey Samples of Bangladesh

Abstract

Honey is a sweet substance made from the nectar of flowers and other chemical secretions from the bees' bodies who collect nectar from the flowers and bring it to their hives to transform it to the thick, golden and sweet liquid that we call honey. The benefits of honey are not just limited to its basic use as a natural sweetener, but also its medicinal properties. The purpose of this study was to identify the bacteria that are present in honey commonly found in Bangladesh, which can tolerate the antimicrobial conditions of honey and survive in it. Fortunately, such bacteria could be detected, isolated and characterized by morphological and biochemical tests. The predominant type of bacteria commonly found in both raw and commercial honeys of Bangladesh are gram positive cocci such as streptococci, staphylococci, micrococci, bacilli and lactobacilli.Few gram negative bacteria were also isolated like Escherichia coli (8%) and Klebsiella pneumonia (8%) and some gram negative/gram variable Micrococcus luteus (75%). Hopefully, such knowledge would benefit people in the future as they will know more about the microorganisms present in honey and about the safety and quality of the honey they are about to buy or consume. Using the Dabur honey sample Micrococcus luteus, Staphylococcus aureus, Streptococcus and bacilluscereus were identified. Five colonies/CFUs were seen on the first and second dilution plates.2. Using the commercial AP Black cumin honey the bacteria identified were Micrococcus luteus, Staphylococcus aureus and Streptococcus. A hundred colonies/CFUs were observed on the first dilution plate and forty-eight CFUs on the second dilution plate. The third had seven CFUs. The commercial AP mixed honey had Micrococcus luteus, Staphylococcus aureus, Streptococcus andKlebsiella pneumonia. The following day, the plates were taken out and four CFUs were observed on the first and second dilution plate and one CFU on the third. 4. The bacteria identified after isolation from the Musaffa khalsi flower honey were Micrococcus luteus, Staphylococcus aureus and Streptococcus. Seventeen CFUs were present on the first dilution plate and sixteen CFUs on the second and third dilution plates.

Antibacterial potential of hive bees honey from Himachal Pradesh, India

“…Our findings were in line with those of Saha et al (2018), who reported that on the basis of morphological and biochemical characteristics, the bacterial isolates encountered in different honeys belong to genera Streptococcus, Staphylococcus, Micrococcus, Bacillus, Lactobacillus, Escherichia, Klebsiella and Micrococcus. The bacterial isolates recovered from the honey collected from Khyber (Pakistan) were identified as Micrococcus luteus, Staphylococcus aureus, Streptococcus pyogenes, S. faecalis, S. epidermidis, Corynebacterium sp., Klebsiella pneumoniae, Escherichia coli, Salmonella sp.…”

Antibiotic Resistant and Plasmid Borne Bacteria Associated with Locally Produced Honey in Enugu State, Nigeria

“…with a total of 4.54% in just a sample. This is in line with Sarbojoy S et al, [14] that identified Escherichia coli (8%), Klebsiella pneumonia (8%), Micrococcus luteus (75%), Streptococci spp., Staphylococci spp., and lactobacilli from honey contaminants in Bangladesh. This could be that these bacteria thrive as contaminants from adulteration of the honey by the producers/sellers.…”

Study of Aerobic Bacteria Isolated from Pus Samples in Tertiary Care Hospital

“…[2] Antimicrobial resistance (AMR), particularly in Grams negative bacteria, has become a prominent worldwide public health concern due to the limited availability of effective treatment alternatives. [3]. Infections caused by resistant organisms have been shown to be associated with elevated death rates and heightened economic burdens.…”