Isolation, drug resistance and molecular characterization of Salmonella isolates in northern Morocco

Abstract

Objectives: The objective of this investigation was to assess the methods for the characterization of Salmonella isolates and to identify relationships of Salmonella isolates from human and food sources in northern Morocco. Methodology: Several Salmonella serotypes were isolated from human and food samples and were characterized using conventional culture methods, biochemical, serological, antimicrobial testing, and phage typing. Molecular analyses such as enterobacterial repetitive intergenic consensus (ERIC)-PCR, macrorestriction profiling by pulsed-field gel electrophoresis (PFGE), and virulence gene analysis were also performed. Results: Sixteen Salmonella strains were isolated in our laboratory, serotyped and identified as S.

Prevalence, antimicrobial susceptibility, and molecular characterization by PCR and pulsed field gel electrophoresis (PFGE) of Salmonella spp. isolated from foods of animal origin in San Luis, Argentina

“…Several studies indicate that PFGE may offer an improved level of discrimination over other genotypic methods for the epidemiological characterization of Salmonella spp. The use of PFGE in combination with XbaI DNA restriction has been widely recognized as a reference method for fingerprinting Salmonella serovars (Ammari et al, 2009a;Kalender, Sen, Hasman, Hendriksen, & Aarestrup, 2009).…”

A molecular epidemiological investigation of multistate outbreaks ofSalmonella Enteritidis from clinical and environmental samples in Turkey, 2000–2010

“…In 2006, 121 Salmonella outbreaks took place in the United States, causing more than 3300 cases reported to the Centers for Disease Control and Prevention (CDC) foodborne outbreak reporting system (2). Salmonella causes estimated millions of illnesses and hundreds of deaths annually in many countries (3). According to the CDC, in 2009 and 2010, a total of 1527 foodborne disease outbreaks (675 in 2009 and 852 in 2010) were reported and Salmonella was the second highest cause, accounting for 30% of outbreaks (4).…”

“…International travel and contact with reptiles have also been associated with S. Enteritidis infection (5). S. Enteritidis isolates have been characterized with phenotyping and genotyping methods in outbreak research (3). Nevertheless, phenotypic methods are not very effective for epidemiological analysis of Salmonella transmission because they cannot discriminate accurately between isolates that are closely related (7).…”