Isolation, expansion, and differentiation of goat adipose-derived stem cells

Ren, Yu; Wang, Haiqing; Zhou, Xueyuan; Wen, Jie; Jin, Minghui; Cang, Ming; Guo, Xudong; Wang, Qinglian; Liu, Dongjun; Ma, Yuzhen

doi:10.1016/j.rvsc.2011.08.014

Cited by 60 publications

(57 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Variable number of stem cells is present in different tissues such as liver, heart, kidney, skin, bone marrow, adipose tissue, placenta, dental, amniotic fluid, amnion and umbilical cord blood as well as in various fetal tissues (Wei et al 2000;Banas et al 2007). Earlier it was considered that stem cells can only differentiate into mature cells of the same organ but recent evidences have shown that they can also differentiate into other cell types and even into the cells of ectoderm, mesoderm and endoderm (Beltrami et al 2001;De Ugarte et al 2003;Jin et al 2007;Vieira et al 2010;Ren et al 2012). It has been demonstrated that MSCs include a heterogeneous population of cells with multilineage differentiation potential, ability to modulate oxidative stress, and produce a variety of factors that are capable of manipulating a broad range of biological functions (Caplan 2007).…”

Section: Characterization and Application Of Adscsmentioning

confidence: 99%

“…Human MSCs can differentiate into other mesodermal tissues such as skeletal muscle (Dezawa et al 2005), myocardium (Shim et al 2004), smooth muscle (Jeon et al 2006), and endothelium (Oswald et al 2004). Herrera et al (2004) (Ren et al 2012). Adult stem cells (ASCs) can be derived from different sites other than the bone marrow.…”

Section: Characterization and Application Of Adscsmentioning

confidence: 99%

“…The results of this study indicated that in order to obtain more and higher quality cells, ADSCs could be subcultured every 14 days instead of the normal five days. ADSCs possess similar surface markers and differentiation potential as BM-MSCs, and can differentiate into cell types of the three germ layers under appropriate conditions (Ren et al 2012). The differentiation potential of ASCs into cells of ecto-and endodermal origin has also been shown (Baer et al 2012).…”

Section: Characterization and Application Of Adscsmentioning

confidence: 99%

“…Therefore, the International Society for Cellular Therapy proposed three minimal criteria for the definition of cultured MSCs: (a) plastic adherence, (b) expression of CD73, CD90, and CD105 and lack of CD34, CD45, CD11b or CD14, CD19 or CD79α and HLA-DR expression, and (c) their trilineage differentiation potential into adipocytes, chondrocytes, and osteoblasts (Oedayrajsingh Varma et al 2007). The immunophenotype of MSCs changes over the course of culturing; some of these changes may represent alterations in the biological features of MSCs; for example, in murine BM-MSCs the loss of expression of CD90, CD15 and CD309 associated with the spontaneous neoplastic transformation after numerous passages (Ren et al 2012). Human MSCs have largely been shown to be free of such transformative events (Ren et al 2012).…”

Section: Characterization and Application Of Adscsmentioning

confidence: 99%

See 3 more Smart Citations

Isolation, Culturing, Characterization and Aging of Adipose Tissue-derived Mesenchymal Stem Cells: A Brief Overview

Fathi

Farahzadi

2016

Braz. arch. biol. technol.

View full text Add to dashboard Cite

The aim of this review was to describe the current state-of-the-art regarding isolation, characterization and aging of adipose tissue-derived mesenchymal stem cells (ADSCs). Mesenchymal stem cells (MSCs) have recently received widespread attention because of their potential use in tissue-engineering applications. Various studies have indicated that MSCs with a fibroblast-like morphology migrate to the sites of injury and help to regenerate damaged tissue. Over the past few years, it has been recognized that fat is not only an energy supply, but also a rich source of multipotent stem cells that can be easily harvested, isolated and selected as compared with other tissues. ADSCs are particularly interesting because of their rapid proliferation and multidirectional differentiation potential.

show abstract

Section: Characterization and Application Of Adscsmentioning

confidence: 99%

Section: Characterization and Application Of Adscsmentioning

confidence: 99%

Section: Characterization and Application Of Adscsmentioning

confidence: 99%

Section: Characterization and Application Of Adscsmentioning

confidence: 99%

See 2 more Smart Citations

Isolation, Culturing, Characterization and Aging of Adipose Tissue-derived Mesenchymal Stem Cells: A Brief Overview

Fathi

Farahzadi

2016

Braz. arch. biol. technol.

View full text Add to dashboard Cite

show abstract

“…Moreover, platelet derived growth factor receptor alpha (PDGFRα) has recently been shown to distinguish adipogenic from non adipogenic cells among interstitial cells in murine muscle (Uezumi et al, 2010). Because available tools have been rather limited until recently in livestock species (Rozemuller et al, 2010), few data have been obtained in pigs (Perruchot et al, 2013) and ruminants (Ren et al, 2012;Sampaio et al, 2015). Clear advancement was allowed by the selection of antibodies against CD34, CD45, CD31, CD56, CD11b, CD14, CD90 and PDGFRα markers in porcine adipose tissue and skeletal muscle (Perruchot et al, 2013).…”

Section: Louveau Perruchot Bonnet and Gondretmentioning

confidence: 99%

Invited review: Pre- and postnatal adipose tissue development in farm animals: from stem cells to adipocyte physiology

Louveau

Perruchot

Bonnet

et al. 2016

Animal

View full text Add to dashboard Cite

Both white and brown adipose tissues are recognized to be differently involved in energy metabolism and are also able to secrete a variety of factors called adipokines that are involved in a wide range of physiological and metabolic functions. Brown adipose tissue is predominant around birth, except in pigs. Irrespective of species, white adipose tissue has a large capacity to expand postnatally and is able to adapt to a variety of factors. The aim of this review is to update the cellular and molecular mechanisms associated with pre-and postnatal adipose tissue development with a special focus on pigs and ruminants. In contrast to other tissues, the embryonic origin of adipose cells remains the subject of debate. Adipose cells arise from the recruitment of specific multipotent stem cells/progenitors named adipose tissue-derived stromal cells. Recent studies have highlighted the existence of a variety of those cells being able to differentiate into white, brown or brown-like/beige adipocytes. After commitment to the adipocyte lineage, progenitors undergo large changes in the expression of many genes involved in cell cycle arrest, lipid accumulation and secretory functions. Early nutrition can affect these processes during fetal and perinatal periods and can also influence or pre-determinate later growth of adipose tissue. How these changes may be related to adipose tissue functional maturity around birth and can influence newborn survival is discussed. Altogether, a better knowledge of fetal and postnatal adipose tissue development is important for various aspects of animal production, including neonatal survival, postnatal growth efficiency and health.

show abstract

Graphene supports in vitro proliferation and osteogenic differentiation of goat adult mesenchymal stem cells: potential for bone tissue engineering

Elkhenany

Amelse

Lafont

et al. 2014

J of Applied Toxicology

133

View full text Add to dashboard Cite

Current treatments for bone loss injuries involve autologous and allogenic bone grafts, metal alloys and ceramics. Although these therapies have proved useful, they suffer from inherent challenges, and hence, an adequate bone replacement therapy has not yet been found. We hypothesize that graphene may be a useful nanoscaffold for mesenchymal stem cells and will promote proliferation and differentiation into bone progenitor cells. In this study, we evaluate graphene, a biocompatible inert nanomaterial, for its effect on in vitro growth and differentiation of goat adult mesenchymal stem cells. Cell proliferation and differentiation are compared between polystyrene-coated tissue culture plates and graphene-coated plates. Graphitic materials are cytocompatible and support cell adhesion and proliferation. Importantly, cells seeded on to oxidized graphene films undergo osteogenic differentiation in fetal bovine serum-containing medium without the addition of any glucocorticoid or specific growth factors. These findings support graphene's potential to act as an osteoinducer and a vehicle to deliver mesenchymal stem cells, and suggest that the combination of graphene and goat mesenchymal stem cells provides a promising construct for bone tissue engineering.

show abstract

Isolation, expansion, and differentiation of goat adipose-derived stem cells

Cited by 60 publications

References 25 publications

Isolation, Culturing, Characterization and Aging of Adipose Tissue-derived Mesenchymal Stem Cells: A Brief Overview

Isolation, Culturing, Characterization and Aging of Adipose Tissue-derived Mesenchymal Stem Cells: A Brief Overview

Invited review: Pre- and postnatal adipose tissue development in farm animals: from stem cells to adipocyte physiology

Graphene supports in vitro proliferation and osteogenic differentiation of goat adult mesenchymal stem cells: potential for bone tissue engineering

Contact Info

Product

Resources

About