Isolation, expression and function analysis of a bZIP transcription factor IbbZIP37 in sweetpotato (Ipomoea batatas L. [Lam])

Wang, Xiangpo Qiu Wenbin; Yang, Yilin; Kim, Ho-Soo; Jia, Huan Yu Xiaoyun; Kwak, Sang‐Soo

doi:10.9755/ejfa.2019.v31.i2.1917

Cited by 2 publications

(1 citation statement)

References 34 publications

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“…In another study, sucrose transporter gene IbSUT4 behaves through the ABF-dependent ABA signaling pathway and plays critical roles under abiotic stresses [ 63 ]. ABA-induced expression of IbbZIP37 and IbMIPS1 under various environmental stresses thus enhances the salt and drought tolerance under field conditions [ 64 , 65 ]. The OE of different sweet potato genes, i.e., IbSPCP2 , IbNFU1 , IbP5CR , IbMas, IbPSS1, and IbMYB308 , significantly enhances salt and drought tolerance in transgenic plants by protecting membrane integrity, regulating osmotic balance, photosynthesis, maintaining Na + homeostasis, Na + exclusion in the root, and activating the ROS scavenging system [ 66 – 69 ] ( Fig.…”

Section: The Functional Genomics Studies For Resilience Against Abiot...mentioning

confidence: 99%

A comprehensive overview of omics-based approaches to enhance biotic and abiotic stress tolerance in sweet potato

Ahmed,

Khan,

Xue

et al. 2024

Horticulture Research

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Biotic and abiotic stresses negatively affect the yield and overall plant developmental process, thus causing substantial losses in global sweet potato production. To cope with stresses, sweet potato has evolved numerous strategies to tackle ever-changing surroundings and biological and environmental conditions. The invention of modern sequencing technology and the latest data processing and analysis instruments has paved the way to integrate biological information from different approaches and helps to understand plant system biology more precisely. The advancement in omics technologies has accumulated and provided a great source of information at all levels (genome, transcript, protein, and metabolite) under stressful conditions. These latest molecular tools facilitate us to understand better the plant's responses to stress signaling and help to process/integrate the biological information encoded within the biological system of plants. This review briefly addresses utilizing the latest omics strategies for deciphering the adaptive mechanisms for sweet potatoes' biotic and abiotic stress tolerance via functional genomics, transcriptomics, proteomics, and metabolomics. This information also provides a powerful reference to understand the complex, well-coordinated stress signaling genetic regulatory networks and better comprehend the plant phenotypic responses at the cellular/molecular level under various environmental stimuli, thus accelerating the design of stress-resilient sweet potato via the latest genetic engineering approaches.

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Section: The Functional Genomics Studies For Resilience Against Abiot...mentioning

confidence: 99%

A comprehensive overview of omics-based approaches to enhance biotic and abiotic stress tolerance in sweet potato

Ahmed,

Khan,

Xue

et al. 2024

Horticulture Research

View full text Add to dashboard Cite

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RNA-sequencing analysis revealed genes associated drought stress responses of different durations in hexaploid sweet potato

Arisha

Ahmad

Tang

et al. 2020

Sci Rep

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Purple-fleshed sweet potato (PFSP) is an important food crop, as it is a rich source of nutrients and anthocyanin pigments. Drought has become a major threat to sustainable sweetpotato production, resulting in huge yield losses. Therefore, the present study was conducted to identify drought stressresponsive genes using next-generation (NGS) and third-generation sequencing (TGS) techniques. Five cDNA libraries were constructed from seedling leaf segments treated with a 30% solution of polyethylene glycol (PEG-6000) for 0, 1, 6, 12, and 48 h for second-generation sequencing. Leaf samples taken from upper third of sweet potato seedlings after 1, 6, 12, and 48 h of drought stress were used for the construction of cDNA libraries for third-generation sequencing; however, leaf samples from untreated plants were collected as controls. A total of 184,259,679 clean reads were obtained using second and third-generation sequencing and then assembled into 17,508 unigenes with an average length of 1,783 base pairs. Out of 17,508 unigenes, 642 (3.6%) unigenes failed to hit any homologs in any databases, which might be considered novel genes. A total of 2, 920, 1578, and 2,418 up-regulated unigenes and 3,834, 2,131, and 3,337 down-regulated unigenes from 1 h, 6 h, 12 h, and 48 h library were identified, respectively in drought stress versus control. In addition, after 6, 12, and 48 h of drought stress, 540 up-regulated unigenes, 486 down-regulated unigenes and 414 significantly differentially expressed unigenes were detected. It was found that several gene families including Basic Helix-loop-helix (bHLH), basic leucine zipper (bZIP), Cystein2/Histidine2 (C2H2), C3H, Ethylene-responsive transcription factor (ERF), Homo domain-leucine zipper (HD-ZIP), MYB, NAC (NAM, ATAF1/2, and CUC2), Thiol specific antioxidant and WRKY showed responses to drought stress. In total, 17,472 simple sequence repeats and 510,617 single nucleotide polymorphisms were identified based on transcriptome sequencing of the PFSP. About 96.55% of the obtained sequences are not available online in sweet potato genomics resources. Therefore, it will enrich annotated sweet potato gene sequences and enhance understanding of the mechanisms of drought tolerance through genetic manipulation. Moreover, it represents a sequence resource for genetic and genomic studies of sweet potato. Purple-fleshed sweet potato (PFSP) (Ipomoea batatas (L.) Lam.) is a type of sweet potato, which has purple skin and flesh color of the storage roots due to the large accumulation of anthocyanin pigments 1. It is a root crop widely grown in some Asian and African countries (i.e. China, India, and Kenya). Sweet potato is regarded as the seventh most important food crop. It is among the crops which generate large amounts of food per unit area per unit time 2. Furthermore, it is considered to be a cheap source of energy, carbohydrates, vitamin, potassium, iron, fiber, and protein 3 .

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Isolation, expression and function analysis of a bZIP transcription factor IbbZIP37 in sweetpotato (Ipomoea batatas L. [Lam])

Cited by 2 publications

References 34 publications

A comprehensive overview of omics-based approaches to enhance biotic and abiotic stress tolerance in sweet potato

A comprehensive overview of omics-based approaches to enhance biotic and abiotic stress tolerance in sweet potato

RNA-sequencing analysis revealed genes associated drought stress responses of different durations in hexaploid sweet potato

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