Isolation, Fractionation and Characterization of Melanin‐like Pigments from Chestnut (<i>Castanea mollissima</i>) Shells

Yao, Zeng-Yu; Qi, Jianhua; Wang, Lihua

doi:10.1111/j.1750-3841.2012.02714.x

Cited by 61 publications

(65 citation statements)

References 31 publications

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“…As shown in Fig. 2, both MAKS and the synthetic melanin showed an absorption maximum at about 200 nm, but a progressive decrease in absorbance with increasing wavelength after 200 nm, in accordance with most previous analyses of melanin 1,32 . The slopes of linear plots are often used to identify melanin.…”

Section: Uv-vis Absorption Spectrasupporting

confidence: 69%

“…0.3 g) of the MAKS were acquired using a Bruker AVANCE III 400 MHz spectrometer (Bruker BioSpin GmbH, Rheinstetten, Germany), operating at a frequency of 100.369 MHz, equipped with a zirconium oxide rotor (external diameter, 7 mm, spun at 5 kHz in a magic-angle spinning, MAS, probe). The crosspolarization contact time, pulse delay, and pulse number were 2.0 ms, 1 s, and 10 000, respectively 32 .…”

Section: C-nmr Analysismentioning

confidence: 99%

“…To determine the UV-Vis absorption spectra of MAKS and of the synthetic melanin, one sample of each was dissolved in ammonia at 0.2%, with ultrasonic assistance, and then the ammonia was removed using a rotary evaporator 21,32 . The resulting solutions were scanned in a U-3310 UV-Vis spectrophotometer (Hitachi, Japan) to obtain absorption spectra at wavelengths ranging from 190-700 nm.…”

Section: Uv-vis Absorption Spectra Of the Melaninsmentioning

confidence: 99%

“…MAKS was dissolved in ammonia at 0.2%, with ultrasonic assistance, then ammonia in the sample was removed using a rotary evaporator 21,32 . After filtering the resulting solution through a 0.45 µm syringe filter, MAKS was separated on a Superdex G-75 column (1.6 × 40 cm) by gel chromatography in 50 mM phosphate buffer (pH 7.5) at a flow rate of 0.5 ml/min.…”

Section: Evaluation Of Molecular Massmentioning

confidence: 99%

“…Infrared spectra of both melanins were recorded, from 4000 to 400 cm −1 , using an FT-IR330 infrared spectrophotometer (Thermo Electron Corporation, U.S.A) with KBr powder 21,32 .…”

Section: Infrared Spectra Of the Melaninsmentioning

confidence: 99%

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Characterization of melanin extracted from apricot (Armeniaca sibirica) and its effect on hydrazine-induced rat hepatic injury

Li¹,

Li²,

Zhao³

2016

ScienceAsia

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ABSTRACT:Melanin is a complex, highly coloured natural antioxidant with potent biological activities and diverse applications. A potential source of melanin is the Siberian apricot (Armeniaca sibirica) whose kernel skin is currently a waste by-product. To explore its potential, melanin from apricot kernel skin was extracted. The molecular mass was determined by gel chromatography and its structure was examined by elemental analysis, UV-Vis, infrared, and solid-state nuclear magnetic resonance ( 13 C-NMR) spectroscopy. In addition, its protective effect against hydrazineinduced hepatic injury in rats was evaluated. The results showed that melanin has < 5% sulphur (S) content, indicating that it consists mostly of eumelanin. Its UV-Vis, infrared and 13 C-NMR spectra were very similar to those of synthetic melanin, although it had relatively high carboxylate, aliphatic, and phenol-skeleton content. Two distinct fractions (2.4 ± 0.12 and 0. ± 560.1 kDa) were detected. Injections of hydrazine at 65 mg/kg body-weight (b.wt.) doses resulted in significant (p < 0.01) increases in lipid peroxidation (malondialdehyde contents) in liver homogenates and serum alanine aminotransferase and aspartate aminotransferase activities (64%, 69%, and 98% increase relative to the controls, respectively). It also induced significant (p < 0.05 or 0.01) decreases in liver homogenate catalase, glutathione peroxidase, and superoxide dismutase activities and reduced glutathione content (48%, 28%, 7%, and 98% reduction, respectively). Administering melanin prior to hydrazine, at 10 mg/kg b.wt., strongly inhibited these changes and ameliorated histopathological hepatic injury. The results indicated that melanin alleviated hydrazine-induced liver injury by relieving oxidative stress, which may provide a novel protective strategy for protection against liver diseases.

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Section: Uv-vis Absorption Spectrasupporting

confidence: 69%

Section: C-nmr Analysismentioning

confidence: 99%

Section: Uv-vis Absorption Spectra Of the Melaninsmentioning

confidence: 99%

Section: Evaluation Of Molecular Massmentioning

confidence: 99%

“…Infrared spectra of both melanins were recorded, from 4000 to 400 cm −1 , using an FT-IR330 infrared spectrophotometer (Thermo Electron Corporation, U.S.A) with KBr powder 21,32 .…”

Section: Infrared Spectra Of the Melaninsmentioning

confidence: 99%

See 3 more Smart Citations

Characterization of melanin extracted from apricot (Armeniaca sibirica) and its effect on hydrazine-induced rat hepatic injury

Li¹,

Li²,

Zhao³

2016

ScienceAsia

View full text Add to dashboard Cite

show abstract

Characterization and biocompatibility of chestnut shell fiber–based composites with polyester

Hsu

Liao

et al. 2014

J of Applied Polymer Sci

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The structural, mechanical, biocompatibility, and biodegradability properties of composite materials formed of poly(butylene succinate) (PBS) and natural fiber (chestnut shell fiber; CSF) were evaluated. Maleic anhydride-grafted poly(butylene succinate) (PBS-g-MA) and treated (crosslinked) CSF (TCSF) were used to improve the mechanical properties of PBS/CSF composites. The results show that PBS-g-MA/TCSF composites have superior mechanical properties compared with both pure PBS and PBS/CSF composites, which is attributed to better compatibility between the polymer and TCSF. Normal human foreskin fibroblasts (FBs) were seeded onto these two series of composites to characterize the biocompatibility. FB proliferation, collagen production, and cytotoxicity assays on the PBS/CSF series of composites exhibited superior results compared with those on the PBS-g-MA/TCSF composites. PBS-g-MA/TCSF was found to be more water resistant than PBS/CSF, and the weight loss of both the composites buried in soil compost indicated that both were biodegradable, especially at high levels of CSF substitution.

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Synthesis and characterization of polydopamine‐derived carbon materials with enhanced thermoelectric performance

Zhu

Wang

et al. 2022

J of Applied Polymer Sci

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Pyrolysis of mussel‐inspired polydopamine (PDA) can yield carbon material with high electrical conductivity and n‐type semiconductor performance. Investigating the relationship between the structure and the thermoelectric properties of carbonized PDA (cPDA) is therefore highly desired. In this work, a series of cPDA annealed at 800°C has been successfully prepared by adjusting the concentration of dopamine (DA) monomers, which had a significant effect on the shape and size of cPDA nanoparticles and their thermoelectric properties. Structural analyses indicate that cPDA are mainly amorphous with some graphite like structure and short‐range ordering. The electrical conductivity and Seebeck coefficient show different trends with the concentration of DA monomers. The largest room‐temperature power factor of 26.15 μW m−1 K−2 was obtained on cPDA when the concentration of DA was 13.12 mM with a conductivity of 28,493 S m−1 and a Seebeck coefficient of −9.58 μV K−1.

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Isolation, Fractionation and Characterization of Melanin‐like Pigments from Chestnut (Castanea mollissima) Shells

Abstract: We provided a new, simple, and inexpensive method to fractionate the melanin-like pigments from chestnut shells. This technique can be used to produce natural melanin-like food colorants with different solubilities from chestnut shells.

Cited by 61 publications

References 31 publications

Characterization of melanin extracted from apricot (Armeniaca sibirica) and its effect on hydrazine-induced rat hepatic injury

Characterization of melanin extracted from apricot (Armeniaca sibirica) and its effect on hydrazine-induced rat hepatic injury

Characterization and biocompatibility of chestnut shell fiber–based composites with polyester

Synthesis and characterization of polydopamine‐derived carbon materials with enhanced thermoelectric performance

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