Isolation from <i>Spirulina</i> membranes of two photosystem I‐type complexes, one of which contains chlorophyll responsible for the 77 K fluorescence band at 760 nm

Shubin, Vladimir; Bezsmertnaya, I. N.; Karapetyan, N. V.

doi:10.1016/0014-5793(92)80803-o

Cited by 62 publications

(41 citation statements)

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“…Spirulina platensis) contain the red chlorophyll (emitting at 760 nm at 77K) that might contribute to the dissipation of energy in PSI trimers (Shubin et al, 1992;Karapetyan et al, 1999). We did not observe the existence of the red chlorophyll species in Synechocystis PCC 6803 in PSI trimers either from the wild type or HLIP deletion mutants.…”

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confidence: 58%

The High Light-Inducible Polypeptides Stabilize Trimeric Photosystem I Complex under High Light Conditions inSynechocystisPCC 6803

et al. 2008

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The high light-inducible polypeptides (HLIPs) are critical for survival under high light (HL) conditions in Synechocystis PCC 6803. In this article, we determined the localization of all four HLIPs in thylakoid protein complexes and examined effects of hli gene deletion on the photosynthetic protein complexes. The HliA and HliB proteins were found to be associated with trimeric photosystem I (PSI) complexes and the Slr1128 protein, whereas HliC was associated with PsaL and TMP14. The HliD was associated with partially dissociated PSI complexes. The PSI activities of the hli mutants were 3-to 4-fold lower than that of the wild type. The hli single mutants lost more than 30% of the PSI trimers after they were incubated in intermediate HL for 12 h. The reduction of PSI trimers were further augmented in these cells by the increase of light intensity. The quadruple hli deletion mutant contained less than one-half of PSI trimers following 12-h incubation in intermediate HL. It lost essentially all of the PSI trimers upon exposure to HL for 12 h. Furthermore, a mutant lacking both PSI trimers and Slr1128 showed growth defects similar to that of the quadruple hli deletion mutant under different light conditions. These results suggest that the HLIPs stabilize PSI trimers, interact with Slr1128, and protect cells under HL conditions.

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The High Light-Inducible Polypeptides Stabilize Trimeric Photosystem I Complex under High Light Conditions inSynechocystisPCC 6803

et al. 2008

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“…Cells of the cyanobacterium Spirulina platensis were grown and the membranes were prepared as described in [9,10]. After Triton X-100 treatment (detergent:Chl = 20 mg/mg), nonsolubilized membranes were removed by centrifugation at 50,000 x g for 40 min at 4°C.…”

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confidence: 99%

Trimeric forms of the photosystem I reaction center complex pre‐exist in the membranes of the cyanobacterium Spirulina platensis

et al. 1993

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Oligomeric and monomeric forms of chlorophyll-protein complexes of photosystem I (PSI) have been isolated from the mesophilic cyanobacterium Spirulina [(1992) FEBS Lett. 309, 340-342]. Electron microscopic analysis of the complexes showed that the oligomeric form is a trimer of the shape and dimensions similar to those of the trimer from thermophilic cyanobacteria. The chlorophyl ratio in the isolated trimer and monomer was found to be 7:3. The trimeric form of PSI complex in contrast to the monomeric one contains the chlorophyll emitting at 760 nm (77K), which is also found in Spirulina membranes and therefore could be used as an intrinsic probe for the trimeric complex. The 77K circular dichroism spectrum of the trimeric form is much more similar to that of Spirulina membranes than the spectrum of the monomer. Thus, the trimeric PSI complexes exist and dominate in the Spirulina membranes.

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“…However, a quenching process at long wavelengths (760 nm) that involves P700+ was detected in trimers and was absent in monomers (Shubin et al, 1992(Shubin et al, , 1995. However, the spectra were normalized to the 726-nm peak because the samples did not have an interna1 standard (Shubin et al, 1992), and the possible quenching of 726-nm fluorescence by P700+ in trimers was not investigated.…”

Section: Discussionmentioning

confidence: 99%

Temporal Changes in State Transitions and Photosystem Organization in the Unicellular, Diazotrophic Cyanobacterium Cyanothece sp. ATCC 51142

1997

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Isolation from Spirulina membranes of two photosystem I‐type complexes, one of which contains chlorophyll responsible for the 77 K fluorescence band at 760 nm

Cited by 62 publications

References 11 publications

The High Light-Inducible Polypeptides Stabilize Trimeric Photosystem I Complex under High Light Conditions inSynechocystisPCC 6803

The High Light-Inducible Polypeptides Stabilize Trimeric Photosystem I Complex under High Light Conditions inSynechocystisPCC 6803

Trimeric forms of the photosystem I reaction center complex pre‐exist in the membranes of the cyanobacterium Spirulina platensis

Temporal Changes in State Transitions and Photosystem Organization in the Unicellular, Diazotrophic Cyanobacterium Cyanothece sp. ATCC 51142

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