Isolation, functional evaluation of probiotic properties and molecular identification of strains isolated from Iranian poultry’s gut

Abstract

Background and Objectives: The use of probiotics as an alternative to antibiotics in poultry diets has attracted a lot of in- terest recently. In this context, the determination of probiotic characteristics was evaluated on several isolates obtained from Iranian poultry’s gut.
 Materials and Methods: Probiotic characteristics such as hemolysis activity, acid, bile, gastric juice tolerate, in vitro ad- hesion assay, cell surface properties (hydrophobicity, auto-aggregation and co-aggregation) and antibiotic susceptibility test were evaluated. Finally, selected isolates identified molecularly after temperature-salt tolerance and extracellular enzyme activity (amylase, protease and cellulose).
 Results: Out of 362 strains isolated from native poultry in three geographical regions of Iran, nine strains (belong to Bacillus sp., Enterococcus sp., Pediococcus sp., Lactobacillus sp., Kluyveromyces sp.) showed resistance against gastrointestinal physiological conditions, desirable surface properties, ability to adhere to epithelial intestine cell line and antibiotic suscep- tibility. Also, these strains were discovered to be temperature-salt tolerant but, only a small number of them were able to produce hydrolase enzymes.
 Conclusion: According to the results, the selected strains can be introduced as native probiotic candidates for utilization in novel poultry feeds.

Genomic characterization and probiotic potential of lactic acid bacteria isolated from feces of guinea pig (Cavia porcellus)

“…Second, the observed halos in this study signify a manifestation of alpha hemolysis on blood agar ( Halder et al , 2017 ). Consistent with these findings, prior investigations have reported analogous hemolytic activity in lactic acid bacteria isolated from the gastrointestinal tracts of poultry and swine ( Tuyarum et al , 2021 ; Makzum et al , 2023 ). The degradation of blood agar is posited to be instigated by hemolysin, an enzymatic agent capable of lysing host cells to facilitate the assimilation of ferrous compounds, particularly hemoglobin ( Chen et al , 2018 ).…”

Characterization and Assessment of Native Lactic Acid Bacteria from Broiler Intestines for Potential Probiotic Properties

“…The aggregation ability of bacteria has usually been connected with adhesion properties [64]. Auto-aggregation facilitates probiotics to adhere to and colonize host intestinal cells, which is critical in several ecological niches [6,30]. According to Roghmann and McGrail [65], auto-aggregation above 40% is necessary for a strain to be a potential probiotic.…”

“…Unquestionably, probiotics continue to be the most promising natural candidates [5]. In response, there has been a surge of interest in employing probiotics as an alternative to antibiotics in livestock and poultry production for disease prevention and enhanced growth performance [6].…”

“…Probiotics are non-pathogenic living microorganisms that confer health benefits when administered in appropriate quantities [7]. They are primarily sourced from dairy products, food, fermented products, animal and human gastrointestinal tracts, and so on [5,6,8]. Lactic acid bacteria (LAB), encompassing various species such as Enterococcus, Lactobacillus, Pediococcus, Streptococcus, Lactococcus, Vagococcus, Leuconostoc, Oenococcus, Weissella, Carnobacterium, and Tetragenococcus, have been extensively researched as probiotics over the years [9].…”

“…Despite the development of a substantial number of commercially available probiotic strains in recent decades, there remains a pressing need for superior strains compared to the existing ones [6]. Moreover, the viability and inconsistent results of probiotic strains in many cases hinder their widespread use [5].…”