Isolation, Genomic Sequence and Physiological Characterization of
            <i>Parageobacillus</i>
            sp. G301, an Isolate Capable of Both Hydrogenogenic and Aerobic Carbon Monoxide Oxidation

Imaura, Yoshinari; Okamoto, Shingo; Hino, Taiki; Ogami, Yusuke; Katayama, Yuka Adachi; Tanimura, Ayumi; Inoue, Masao; Kamikawa, Ryoma; Yoshida, Takashi; Sako, Yoshihiko

doi:10.1128/aem.00185-23

Cited by 7 publications

(4 citation statements)

References 99 publications

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“…In the syngas atmospheres, varying CO levels influenced growth and metabolism to a large extent, impacting downstream H 2 production. H 2 production in these atmospheres showed considerably shorter lag phases between inoculation and onset of the WGS reaction, similar to the anaerobic control and previous anaerobic cultivations that have exhibited reductions to within 4 h post-inoculation [ 19 ]. The 50% syngas, atmosphere, with the lowest CO level (~ 17%), exhibited pronounced earlier onset of fermentative metabolism and H 2 production compared to the anaerobic control (CO level ~ 46%) and the syngas (100%) atmosphere (CO level ~ 38%).…”

Section: Discussionsupporting

confidence: 83%

“…As such, the majority of bacterial taxa capable of conducting the WGS reaction are strict anaerobes. One exception, however, is the facultatively anaerobic thermophile P. thermoglucosidasius , which carries a Ni-CODH/NiFe group 4a hydrogenase complex and is capable of the WGS reaction following oxygen depletion [ 10 , 17 – 19 ]. By optimising growth medium composition and pH, fermentation temperatures and CO concentration, H 2 gas production yields have been improved [ 20 ], but further developments towards industrial application are necessary.…”

Section: Introductionmentioning

confidence: 99%

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The effects of synthesis gas feedstocks and oxygen perturbation on hydrogen production by Parageobacillus thermoglucosidasius

Mol,

Ardila,

Mol

et al. 2024

Microb Cell Fact

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Background The facultatively anaerobic thermophile Parageobacillus thermoglucosidasius is able to produce hydrogen gas (H2) through the water–gas shift (WGS) reaction. To date this process has been evaluated under controlled conditions, with gas feedstocks comprising carbon monoxide and variable proportions of air, nitrogen and hydrogen. Ultimately, an economically viable hydrogenogenic system would make use of industrial waste/synthesis gases that contain high levels of carbon monoxide, but which may also contain contaminants such as H2, oxygen (O2) and other impurities, which may be toxic to P. thermoglucosidasius. Results We evaluated the effects of synthesis gas (syngas) mimetic feedstocks on WGS reaction-driven H2 gas production by P. thermoglucosidasius DSM 6285 in small-scale fermentations. Improved H2 gas production yields and faster onset towards hydrogen production were observed when anaerobic synthetic syngas feedstocks were used, at the expense of biomass accumulation. Furthermore, as the WGS reaction is an anoxygenic process, we evaluated the influence of O2 perturbation on P. thermoglucosidasius hydrogenogenesis. O2 supplementation improved biomass accumulation, but reduced hydrogen yields in accordance with the level of oxygen supplied. However, H2 gas production was observed at low O2 levels. Supplementation also induced rapid acetate consumption, likely to sustain growth. Conclusion The utilisation of anaerobic syngas mimetic gas feedstocks to produce H2 and the relative flexibility of the P. thermoglucosidasius WGS reaction system following O2 perturbation further supports its applicability towards more robust and continuous hydrogenogenic operation.

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Section: Discussionsupporting

confidence: 83%

Section: Introductionmentioning

confidence: 99%

The effects of synthesis gas feedstocks and oxygen perturbation on hydrogen production by Parageobacillus thermoglucosidasius

Mol,

Ardila,

Mol

et al. 2024

Microb Cell Fact

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“…Prokaryotes utilizing CO are called CO utilizers and have been found in various environments, including soil (Suzuki et al 1983), lake (Leigh et al 1981), freshwater lake sediments (Imaura et al 2023), bioreactors (Hattori et al 2000), hot springs (Svetlichny et al 1991), deep hydrothermal vents (Moussard et al 2004), and even gut environment (Trischler et al 2022). All CO utilizers possess CO dehydrogenase (CODH), which catalyzes the reversible conversion of CO 2 and CO (CO 2 + 2H + + 2e − 6 <=> CO + H 2 O) (Oelgeschläger and Rother 2008;Sokolova et al 2009).…”

Section: Introductionmentioning

confidence: 99%

“…1983), lake (Leigh et al . 1981), freshwater lake sediments (Imaura et al . 2023), bioreactors (Hattori et al .…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic diversity of the carbon monoxide-utilizing prokaryotes and their divergent carbon monoxide metabolisms in the human gut microbiome

Katayama,

Kamikawa,

Yoshida

2023

Preprint

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Although the production of toxic CO within the human body has been detected, only a few CO-utilizing prokaryotes (CO utilizers) have been reported in the human gut, and their phylogenetic and physiological diversity remains unclear. Here, we unveiled more than thousand representative genomes originating from previously unexplored potential CO-utilizing prokaryotes, which contain CO dehydrogenase (CODH) genes. More than half of CODH-bearing prokaryotes possess genes for the autotrophic Wood-Ljungdahl pathway (WLP). However, 95% of these prokaryotes commonly lack a key gene for WLP, which encodes enzyme that synthesizes formate from CO2 and H2, suggesting that they share a degenerated WLP. Instead, many were predicted to possess an alternative way of synthesizing formate from pyruvate, which is a product of glycolysis. This suggests that glycolysis may serve as a potential supplier of carbon and reducing power to degenerate WLP, which may no longer function as a complete autotrophic pathway. In addition to degenerated WLP, one of the seven predicted CO metabolic pathways involves CO oxidation, which is linked to nitrate reduction and oxaloacetate synthesis. Our findings reveal the unique and diverse nature of CO metabolism in the human gut microbiome, suggesting its potential contribution to CO consumption and gut homeostasis.

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Construction of multiple metagenome assembled genomes containing carbon monoxide dehydrogenases from anaerobic carbon monoxide enrichment cultures

et al. 2023

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Isolation, Genomic Sequence and Physiological Characterization of Parageobacillus sp. G301, an Isolate Capable of Both Hydrogenogenic and Aerobic Carbon Monoxide Oxidation

Cited by 7 publications

References 99 publications

The effects of synthesis gas feedstocks and oxygen perturbation on hydrogen production by Parageobacillus thermoglucosidasius

The effects of synthesis gas feedstocks and oxygen perturbation on hydrogen production by Parageobacillus thermoglucosidasius

Phylogenetic diversity of the carbon monoxide-utilizing prokaryotes and their divergent carbon monoxide metabolisms in the human gut microbiome

Construction of multiple metagenome assembled genomes containing carbon monoxide dehydrogenases from anaerobic carbon monoxide enrichment cultures

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