Isolation, identification and anti-candidal activity of filamentous fungi from Saudi Arabia soil

Al‐Enazi, Nouf M.; Awaad, Amani S.; Al‐Othman, Monerah R.; Al-Anazi, Nour K.; Alqasoumi, Saleh I.

doi:10.1016/j.jsps.2017.12.003

Cited by 16 publications

(9 citation statements)

References 9 publications

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“…The silver nanoparticles synthesized by wheatgrass aqueous extract were tested for anti-candidal activity against five different clinical isolates of C. albicans MTCC3017, C. albicans MTCC1637, C. albicans MTCC183 C. tropicalis MTCC230 and C. glabrata MTCC3814 (Microbial type culture collection and gene bank, Chandighar, India) using agar well diffusion method [24] . A 20 ml of YPDA (Yeast extract-1 g, peptone-2 g, dextrose-2 g, agar-1.5/100 ml of distilled water) medium was poured into sterilized petri plates and freshly grown 24 h old Candida cultures were seeded on YPDA medium.…”

Section: Anti-candidal Activity Of Silver Nanoparticlesmentioning

confidence: 99%

Biosynthesis and Characterization of Silver Nanoparticles Using Wheatgrass Extract and Assessment of Their Anticandidal Activity

Kamradgi¹,

Anganellikar²,

Babanagare³

et al. 2021

ijps

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Kamradgi et al.: Synthesis of Silver Nanoparticles from Wheatgrass ExtractWheatgrass extract was used for eco-friendly extracellular synthesis of silver nanoparticles. Stable silver nanoparticles were formed by treating the wheatgrass extract with aqueous silver nitrate solution as reducing agents. Ultraviolet-visible spectroscopy was used to confirm the presence of silver nanoparticles. The X-ray diffraction analysis exhibited characteristic peaks signifying the crystalline nature of nanoparticels. The particle size and the involvement of various biomolecules in the synthesis of silver nanoparticles were determined using Atomic force microscopy and Fourier transform infrared spectroscopy, respectively. The particle structure was studied by scanning electron microscopy and the elemental silver present in reaction mixture was confirmed by Energy dispersive x-ray. The nanoparticles were tested for their anticandidal activity against Candida albicans MTCC3017, Candida albicans MTCC1637, Candida albicans MTCC183, Candida tropicalis MTCC230 and Candida glabrata MTCC3814 and determined their minimum inhibitory concentration.

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Section: Anti-candidal Activity Of Silver Nanoparticlesmentioning

confidence: 99%

Biosynthesis and Characterization of Silver Nanoparticles Using Wheatgrass Extract and Assessment of Their Anticandidal Activity

Kamradgi¹,

Anganellikar²,

Babanagare³

et al. 2021

ijps

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“…Antimicrobial activity of the extra-cellular extract of Alternaria alternata isolated from soil against Proteus vulgaris and Salmonella typhi has been reported ( El hamd et al, 2014 ). In another investigation, Penicillium melinii, Petriella setifera, Aspergillus pseudo-niger, Alternaria chlamydospora, Pythium nayoroense, Phoma glomerata, Mucor ramosissimus, Mucor racemosus, Fusarium chlamydosporum and Rhizopus azygosporus were isolated from soil and showed anticandidal activity ( Al-Enazi et al, 2018 ). However, the previous studies identified microbial abundance from sabkha in Saudi Arabia, but soil microbial diversity in desert of Saudi Arabia was not sufficiently covered.…”

Section: Introductionmentioning

confidence: 99%

Microbial Diversity of Some Sabkha and Desert Sites in Saudi Arabia

Alotaibi

Sonbol

Alwakeel

et al. 2020

Saudi Journal of Biological Sciences

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Several studies isolated fungal and bacterial species from extreme environments, such as Sabkha and hot deserts, as their natural habitat, some of which are of medicinal importance. Current research aimed investigating the microbial (fungi and bacteria) diversity and abundance in Sabkha and desert areas in Saudi Arabia. Soil samples from nine different geographical areas (Al-Aushazia lake, AlQasab, AlKasar, Tabuk, Al-Kharj, Al-Madina, Jubail, Taif and Abqaiq) were collected and cultured for microbial isolation. Isolated fungi and bacteria were identified by molecular techniques (PCR and sequencing). Based on 18S rDNA sequencing, 203 fungal species belonging to 33 genera were identified. The most common fungal genera were Fusarium , Alternaria , Chaetomium , Aspergillus Cochliobolus and Pencillium , while the most common species were Chaetomium globosum and Fusarium oxysporum . By 16S rDNA sequencing 22 bacterial species belonging to only two genera, Bacillus and Lactobacillus , were identified. The most commonly isolated bacterial species were Bacillus subtilis and Lactobacillus murinus . Some fungal species were confined to specific locations, such as Actinomyces elegans, Fusarium proliferatum, Gymnoascus reesii and Myzostoma spp . that were only isolated from Al-Aushazia soil. AlQasab soil had the highest microbial diversity among other areas with abundances of 23.5% and 4.4% of total fungi, and bacteria, respectively. Findings of this study show a higher degree of fungal diversity than that of bacteria in all studied areas. Further studies needed to investigate the connection between some isolated species and their habitat ecology, as well as to identify those of medicinal importance.

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“…For isolation of all fungi four different media were used (yeast extract sucrose potato dextrose, czapek’s dox and yeast malt extract agar malt extract) were uses. The technique used was described by Al-Enazi et al (2018).…”

Section: Methodsmentioning

confidence: 99%

“…The antimicrobial activity of internal and external secondary metabolites of the isolated fungal strains grown on malt extract, yeast extract sucrose, and yeast-malt extract media were determined using disc-diffusion method (Al-Enazi et al, 2018). Petri dishes containing 20 mL of agar medium were seeded with test organisms.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of antimicrobial activity of secondary metabolites of fungi isolated from Sultanate Oman soil

Alkhulaifi

Awaad

AL-Mudhayyif

et al. 2019

Saudi Pharmaceutical Journal

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Seven fungal species were isolated from soil samples collected from the University of Sultan Qaboos, Muscat, Sultanate of Oman. The fungal isolates were identified as Aspergillus athecius, A. terreus var. africans, A. flavus, A. terreus, A. foetidus, Fusarium chlamydosporum and F. nygamai. Phytochemical and chromatographic investigation showed variety of secondary metabolites in all of the fugal extracts (extra and intra cellular). The antimicrobial activity of internal and external extracts of the isolated fungal species were screened against Candida albicans, C. glabrata, C. parapsilosis, C. tropicalis, Pseudomonas aeruginosa, Lactobacillus acidophilus, Streptococcus gordonii, S. mutans. The antimicrobial activity of external secondary metabolites was generally better than the internal metabolites. The highest antimicrobial activity (32 mm, 30 mm and 29 mm) was obtained from external secondary metabolites of Aspergillus flavus against Candida tropicals, Candida parapsilosis and Candida albicans, respectively.

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Isolation, identification and anti-candidal activity of filamentous fungi from Saudi Arabia soil

Cited by 16 publications

References 9 publications

Biosynthesis and Characterization of Silver Nanoparticles Using Wheatgrass Extract and Assessment of Their Anticandidal Activity

Biosynthesis and Characterization of Silver Nanoparticles Using Wheatgrass Extract and Assessment of Their Anticandidal Activity

Microbial Diversity of Some Sabkha and Desert Sites in Saudi Arabia

Evaluation of antimicrobial activity of secondary metabolites of fungi isolated from Sultanate Oman soil

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