Isolation, Identification and Antibiogram of Coagulase Negative Staphylococcus (CoNS) Isolated from Various Clinical Samples at a Tertiary Care Teaching Hospital, Jaipur, India

Kumar, S. Dinesh; endra, Jit; Das, Anup Kumar; Mane, Pratibha; Sangwan, Jyoti; Kumari, Saroj

doi:10.20546/ijcmas.2018.701.362

Cited by 4 publications

(4 citation statements)

References 8 publications

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“…In this study, we found 26% of patients with UTI were positive for S. haemolyticus, which is consistent with the previous findings [9]. Surprisingly, a study conducted by Lerbech et al in Ghana reported that S. haemolyticus was the predominant pathogen (75%) in human urine collected from patients with UTI; whereas, others in India found a much lower incidence rate of S. haemolyticus in patients with UTI ranging from 15-18% [24,25,26,27]. The variation in the incidence rate of S. haemolyticus might be due to the factors influencing the variability of collected samples, lack of species identification opportunity in most laboratories, time, antibiotic use, and lack of advanced technologies in most developing countries like Bangladesh.…”

Section: Discussionsupporting

confidence: 91%

“…They suggest genetic evidence that S. haemolyticus shares a shared antimicrobial resistance gene pool with S. epidermidis [9]. On the other hand, in the study among the tested antibiotics, vancomycin was found to be most effective for treating UTI patients against S. haemolyticus [24,25,38,39]. In our study, S. haemolyticus was 100% sensitive to vancomycin followed by azithromycin (80.76%), amikacin (84.61%), gentamycin (69.23%), levofloxacin (73.08%), ceftriaxone (80.76%) and doxycycline (61.54%), respectively.…”

Section: Discussionmentioning

confidence: 96%

“…In developing countries, including Bangladesh, the most traditional way of bacteria identification is to isolate the organism and to study its phenotype through culture, biochemical tests, and Gram staining. Similar studies were conducted in India, Iran, Turkey and Indonesia, where they used the conventional method for the identification of causative bacteria from the chronic disease [25][26][27][31][32][33][34]. Although this method is very informative, yet it is time-consuming, inapplicable for non-cultivable bacteria, biochemical tests are not suited for many known genera and species of bacteria and need a skilled person.…”

Section: Discussionmentioning

confidence: 99%

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Molecular Characterization and Antibiogram Profiling of Multidrug Resistant Staphylococcus haemolyticus Isolated from Patients with Urinary Tract Infection in Bangladesh

MH¹,

ML²,

Sarker³

et al. 2021

jbacteriolmycol

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The emergence of antibiotic-resistant bacteria in human is a potential global public health concern. Profiling of antibiotic-resistant bacteria with their antimicrobial susceptibility patterns from Urinary Tract Infections (UTIs) is crucial to guide antibiotic therapy. Herein we report a detailed bacteriological and molecular analysis of Staphylococcus haemolyticus and their antibiogram typing from UTIs. A total of 100 human urine samples of patients with UTIs were collected between January and December 2019 and were subjected to the conventional characterization of bacteria using the standard protocol. Molecular characterization was performed via sequencing followed by phylogenetic analysis. All bacterial strains were examined against thirteen commonly used antibiotics for susceptibility using the Kirby-Bauer disk diffusion method. The overall prevalence of S. haemolyticus in UTI was 26% with female patients having a higher prevalence of UTI (21 out of 26 or 80.76%) than male patients (5 out of 26 or 19.24%). The isolated S. haemolyticus showed 100%, 100%, 88.46%%, 76.93%, 73.08% and 65.39% resistant to penicillin, ampicillin, amoxicillin, erythromycin, ciprofloxacin and tetracycline, respectively. Importantly, S. haemolyticus demonstrated the highest sensitivity to vancomycin (100%), followed by azithromycin (80.76%), amikacin (84.61%), gentamycin (69.23%), levofloxacin (73.08%), ceftriaxone (80.76%) and doxycycline (61.54%). Overall, six variations were noted in S. haemolyticus in which most (5/6) modifications were substitutions and one (1/6) was deletion. These findings imply that mutations in the 16S rRNA gene sequence are the dominant source for species identification and variation in the drug sensitivity pattern against the S. haemolyticus. Phylogenetic analysis of the resultant 16S rRNA indicated that the isolated S. haemolyticus in this study belonged to genus Staphylococcus, but was different from the rest of the available S. haemolyticus isolates in other countries. Multidrug-resistant pathogenic S. hemolyticus is commonly found in urine samples of UTI in human in Bangladesh, which warrants a one-health approach for controlling this emerging ailment.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

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Molecular Characterization and Antibiogram Profiling of Multidrug Resistant Staphylococcus haemolyticus Isolated from Patients with Urinary Tract Infection in Bangladesh

MH¹,

ML²,

Sarker³

et al. 2021

jbacteriolmycol

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show abstract

“…In general, the species most commonly found in nosocomial infections is S. epidermidis, followed by S. haemolyticus, S. hominis, and S. capitis [53] [59] [60].…”

Section: Most Common Species Of Cons Found In Nosocomial Infectionsmentioning

confidence: 99%

Pathogenicity and Antimicrobial Resistance in Coagulase-Negative Staphylococci

Goulart¹

2023

JBM

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The coagulase-negative staphylococci (CoNS) group was considered saprophytic or rarely pathogenic for many years. Since the first case of septicemia caused by CoNS, there has been a progressive increase in the prevalence of healthcare-associated infections caused by CoNS. The CoNS group has emerged as one of the main causes of nosocomial infections related to vascular catheters and prostheses, especially among immunocompromised patients. This gradual increase in infections is due to the change in the relationship between patients and procedures since CoNS are closely related to devices implanted in the human body. CoNS are successful in colonizing the host because they have several virulence mechanisms, such as biofilm formation and production of enzymes and toxins, in addition to several mechanisms of resistance to antimicrobials. Despite their great clinical relevance, few studies have focused on CoNS's pathogenicity and resistance to antimicrobials, which reveals the current need to better understand the factors by which this group became pathogenic to humans and other animals. This review aims to synthesize the aspects related to the pathogenicity and antimicrobial resistance in CoNS.

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Resistencia antimicrobiana en staphylococcus aureus y staphylococcus epidermidis : tendencia temporal (2010-2016) y fenotipos de multirresistencia, Cartagena (Colombia).

Orozco

Ferrer²,

Jiménez³

et al. 2018

biosalud

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Introducción: Las especies de Staphylococcus spp. son patógenos oportunistas que causan enfermedades como osteomielitis y bacteriemias. Estas bacterias pueden adquirir resistencia a antibióticos, lo que hace que se conviertan en un problema de salud pública debido a la restricción de opciones terapéuticas. Objetivo: Evaluar la tendencia de los perfiles de resistencia antimicrobiana de S. aureus y S. epidermidis aislados en un hospital de Cartagena entre 2010 y 2016. Materiales y métodos: Estudio de corte transversal. El método de microdilución en caldo fue usado para la determinación de la concentración mínima inhibitoria de 12 agentes antimicrobianos. Se estudió el comportamiento de la resistencia antimicrobiana de acuerdo a la especie Staphylococcus, el sitio de infección y el período de tiempo. Resultados: Se aislaron 1218 cocos grampositivos, de los cuales 42,7 % fueron S. aureus y 18,9 % S. epidermidis. El 47,5 % de S. aureus fueron resistentes a meticilina y se encontraron principalmente en secreciones (43,3 %); mientras que el 68,7 % de S. epidermidis fueron meticilino resistentes y aislados principalmente en sangre (76,9 %). Conclusión: Se identificaron aislamientos clínicos de S. aureus y S. epidermidis con perfil de multirresistencia. Se observó un comportamiento constante en sus perfiles de resistencia durante el período de estudio, excepto en los dos últimos años en los que se identificó una reducción significativa de la meticilino resistencia en S. epidermidis.

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Isolation, Identification and Antibiogram of Coagulase Negative Staphylococcus (CoNS) Isolated from Various Clinical Samples at a Tertiary Care Teaching Hospital, Jaipur, India

Cited by 4 publications

References 8 publications

Molecular Characterization and Antibiogram Profiling of Multidrug Resistant Staphylococcus haemolyticus Isolated from Patients with Urinary Tract Infection in Bangladesh

Molecular Characterization and Antibiogram Profiling of Multidrug Resistant Staphylococcus haemolyticus Isolated from Patients with Urinary Tract Infection in Bangladesh

Pathogenicity and Antimicrobial Resistance in Coagulase-Negative Staphylococci

Resistencia antimicrobiana en staphylococcus aureus y staphylococcus epidermidis : tendencia temporal (2010-2016) y fenotipos de multirresistencia, Cartagena (Colombia).

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