Isolation, identification, and biochemical characterization of a brown rot fungus capable of textile dye decolorization

Gomaa, Ola M.; Momtaz, Osama A.; Kareem, Hussein Abd El; Fathy, Riham

doi:10.1007/s11274-010-0618-x

Cited by 7 publications

(7 citation statements)

References 32 publications

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“…All primers used for DNA amplification reactions were purchased from Integrated DNA Technologies. Bacillus isolates characterized biochemically were reconfirmed by PCR targeting 16SrRNA (Gomaa and Momtaz 2006). PCR mixture (25 ul) contained, template DNA (5 ll), GoTaq Green Master Mix (12.5ul, Thermo Fisher Scientific, Mumbai, India), nuclease free water (6.5ul, Thermo Fisher Scientific, Mumbai, India), 0.5ul of forward primer (10 pmol/ll), 0.5 ull of reverse primer (10 pmol/ll).…”

Section: Dna Extraction and Amplification Reactionsmentioning

confidence: 99%

Isolation, characterization and antibiogram ofBacillus cereusfrom milk products

Yadav,

Khurana,

Kumar

et al. 2024

Preprint

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This study, conducted within the Department of Veterinary Public Health and Epidemiology (VPHE) at Lala Lajpat Rai University of Veterinary and Animal Sciences (LUVAS), undertook a comprehensive investigation into the prevalence, identification, characterization, and antibiotic resistance patterns of Bacillus cereus in milk products from various regions of Haryana, India. Using a systematic sampling strategy, eight tehsils spanning two agroclimatic zones were selected for sample collection. A total of 200 samples were obtained from randomly selected shops within these tehsils. Each sample underwent pre-enrichment in Brain Heart Infusion (BHI) broth with a 1:10 dilution to facilitate the growth of any existing B. cereus contaminants. Following pre-enrichment, streaking on selective PEMBA agar plates was performed for the isolation of B. cereus colonies. The presence of presumptive B. cereus colonies was confirmed through a series of biochemical tests, including gram staining, nitrate reduction, oxidase, indole, methyl red reduction, Voges Praskaur, and catalase tests. Molecular analysis using the 16SrRNA gene confirmed the presence of 88 positive B. cereus isolates. Further characterization involved the differentiation of B. cereus from Bacillus thuringiensis via the cry2 gene. Additionally, the study assessed the presence of virulence-associated genes, identifying gyrB, cytk, hblA, and nheA genes in the isolated strains. Geographical variation in B. cereus prevalence was observed, with higher rates detected in certain tehsils such as Kalka (68%) and Panchkula (60%). Antibiotic sensitivity testing using Tryptone Soya Agar (TSA) broth for enrichment and Muller Hinton Agar (MHA) plates with 14 antibiotic discs revealed widespread resistance among the isolates. Notably, all isolates exhibited resistance to Ampicillin, Cefazolin, Methicillin, Polymyxin-B, and Penicillin, while displaying maximum sensitivity to Amikacin, Gentamycin, Levofloxacin, and Meropenem. Alarmingly, all isolates displayed multiple drug resistance (MDR), indicating resistance to three or more classes of antibiotics. These findings underscore the urgent need for stringent food safety measures and surveillance protocols in dairy production and distribution. Effective interventions are imperative to mitigate the risk of B. cereus contamination and combat antibiotic resistance in milk products. Collaboration with the VPHE department at LUVAS and continued research efforts are essential to address emerging challenges and uphold food safety standards effectively.

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Section: Dna Extraction and Amplification Reactionsmentioning

confidence: 99%

Isolation, characterization and antibiogram ofBacillus cereusfrom milk products

Yadav,

Khurana,

Kumar

et al. 2024

Preprint

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“…Correlation between the activities of those two enzymes during the biodecolorization of synthetic dyes was demonstrated also by Lan et al (2006) in experiments on the decolorization of fuchsine and rhodamine B by Phanerochaete chrysosporium ATCC 34541. Gomaa et al (2011) observed that the decolorization effectiveness of a strain of Aspergillus sp. toward Basic Blue increased with increasing activity of glucose oxidase.…”

Section: Enzyme Activitiesmentioning

confidence: 99%

Screening of microscopic fungi and their enzyme activities for decolorization and biotransformation of some aromatic compounds

Korniłłowicz-Kowalska

Rybczyńska

2014

Int. J. Environ. Sci. Technol.

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A total of 610 strains from 16 species of micromycetes with potential ligninolytic capabilities were isolated from soil, compost, and rotten wood, including 400 strains of soil origin. In the test of decolorization of agarized 0.06 % alizarin blue, 52 strains with high decolorization activity were selected. In liquid cultures with 0.2 % post-industrial lignin and 0.03 % alizarin blue, all of the selected strains showed three different extracellular peroxidase activities such as HRP-like, LiP, and MnP. Laccase was detected only in the cultures with alizarin blue, but those were trace amounts. In the decolorization of alizarin blue, the highest levels of activity were attained by HRPlike peroxidase. In the transformations of post-industrial lignin that included decolorization, colorization (darkening of substrate), and re-colorization, the highest levels of activity were attained by lignin peroxidase, especially at the start of the culture. The transformations of both dye substrates in the cultures of all 52 strains were accompanied by the release of glucose oxidase, but high activities of that enzyme were observed only in the presence of alizarin blue. Whereas in the presence of lignin the fungi under study synthesized catalase, the activity of which attained a maximum toward the end of the culture was negatively correlated with the activity of the remaining oxidoreductases under study. Among the 52 selected strains, three strains such as Haematonectria haematacocca BwIII43, K37, and Trichoderma harzianum BsIII33 were characterized by a high activity of decolorization.

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“…EL-2 capable of decolorizing textile waste water (Gomaa et al 2011) is used in the following study. The fungus was grown on GYP medium which consisted of glucose (20 g/l); yeast extract (5 g/l); peptone (5 g/l) and magnesium sulfate (1 g/l).…”

Section: Microorganism and Cultivation Conditionsmentioning

confidence: 99%

“…EL-2, characterized in a previous work (Gomaa et al 2011) as a promising and efficient organism in bioremediation of textile waste water, is further investigated in the current work in terms of assessment of the level of toxicity of single and combined treated textile waste water for its potential use in non-potable applications.…”

Section: Introductionmentioning

confidence: 99%

Assessment of the efficacy of Aspergillus sp. EL-2 in textile waste water treatment

2011

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Fungal biomass has the ability to decolorize a wide variety of dyes successfully through a number of mechanisms. A brown rot isolate, previously identified as Aspergillus sp. EL-2, was used in the aerobic treatment of textile waste water efficiently. In the current work, the treated waste water was tested chemically using more than one combined treatment. Microbial toxicity, phytotoxicity, genotoxicity and cytotoxicity were also studied to assess the toxicity level for each treatment. The obtained data suggest that the contribution of more than one mode of treatment is essential to ensure complete destruction of the by-products. The use of gamma irradiation (25 kGy) after the bioremediation step led to the decrease of the by-products of biodegradation as observed by visible spectrum and Fourier transfer infra red spectroscopy (FT-IR). The toxicity assessment presented variable results indicating the need for more than one toxicity test to confirm the presence or absence of hazardous compounds. Brown rot fungus could be used efficiently in the treatment of textile waste water without the risk of obtaining high carcinogenic or genotoxic compounds, especially if combined treatment is employed.

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Isolation, identification, and biochemical characterization of a brown rot fungus capable of textile dye decolorization

Cited by 7 publications

References 32 publications

Isolation, characterization and antibiogram ofBacillus cereusfrom milk products

Isolation, characterization and antibiogram ofBacillus cereusfrom milk products

Screening of microscopic fungi and their enzyme activities for decolorization and biotransformation of some aromatic compounds

Assessment of the efficacy of Aspergillus sp. EL-2 in textile waste water treatment

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