Isolation, identification, and biological evaluation of HIF-1-modulating compounds from Brazilian green propolis

Isolation of Six Isoprenylated Biflavonoids from the Leaves of <i>Garcinia subelliptica</i>

“…32) We observed that the acetone-soluble part of the leaves of G. subelliptica inhibited HIF-1-dependent luciferase activity under hypoxic conditions. The effects of one major biflavonoid (7) on HIF-1 activity were examined further under hypoxic conditions, which we compared with aerobic exposure in the HEK293 clone (Fig.…”

“…The cell culture, hypoxia treatment, and cell-based luciferase reporter assay were performed according to protocols described in a previous study. 32) MTT Assay HEK293 cells were plated at a density of 6.0×10 3 cells/well (96-well plate, TPP Techno Plastic Products AG) in 100 µL of McCoy's 5A medium with 10% FBS and antibiotics, incubated for 24 h, and exposed to the test compounds under aerobic or hypoxic conditions for 24 h. The MTT reagent (0.5 mg/mL, Sigma-Aldrich, Japan) was added to the media. After 4-h incubation at 37°C, the media were removed, and the cells were lysed with DMSO.…”

Chemical profiling of six samples of Brazilian propolis

“…2,3 The data given in Table 2, however, indicate that the flavonoids 20, 21 and 23 number among the major constituents of the propolis samples from Minas Gerais (Table 3). While kaempferide (23) has been reported as an important constituent of Brazilian green propolis, 33 isorhamnetin (21) and derivatives of pinobanksin (such as 20) have rarely or never been detected in this type of propolis. Caffeoylquinic acids have been mentioned as frequent biologically active constituents from aqueous extracts of Brazilian propolis.…”

A flavonoid chrysin suppresses hypoxic survival and metastatic growth of mouse breast cancer cells

“…This has received much attention as an attractive target for anticancer drug development (3). Clinical studies indicate correlation between tumor hypoxia, tumor progression and development of distant metastases (4,5).…”