Isolation, Identification, and Characterization of a Keratin-degrading Bacterium Chryseobacterium sp. P1-3

Abstract

In this study, a keratin-degrading bacterium was isolated from soil contaminated with feather waste. The isolated strain was identified as Chryseobacterium sp. P1-3 on the basis of the 16S rRNA gene sequence alignment. Chryseobacterium sp. P1-3 is currently used in various biotechnological applications (e.g., in the hydrolysis of poultry feathers). It hydrolyzed the feather meal within 2 days and possesses a high level of keratinase activity (98 U/mL). The keratinase, partially purified from this strain, prefers casein as a substrate and shows optimal activity at a temperature of 30 o C and at a pH of 8.0.

Chryseobacterium aquifrigidense FANN1 Produced Detergent-Stable Metallokeratinase and Amino Acids Through the Abasement of Chicken Feathers

“…nov. (Lv et al, 2010), and Chryseobacterium sp. P1-3 (Hong et al, 2015) exhibited optimal catalysis at pH and temperature of 8.5 and 50°C, 7.5 and 45-55°C, 8.0 and 40°C, and 8.0 and 30°C, respectively. The thermostability study showed that the enzyme could be used at moderately high-temperature conditions over a given time with significant biocatalytic efficiency.…”

“…Contrariwise, optimal keratinase production by Chryseobacterium indologenes A22 (Bach et al, 2015), Chryseobacterium sp. P1-3 (Hong et al, 2015) and Chryseobacterium sp. RBT (Gurav et al, 2016) was recorded at 40, 48, and 48 h, respectively.…”

Valorization of Livestock Keratin Waste: Application in Agricultural Fields

“…There are many keratin-hydrolyzing microorganisms that have been isolated from poultry breeding and waste or soil processing, of which the Bacillus is the most abundant. Common keratin-degrading microorganisms include Amycolatopsis [ 51 ], Bacillus [ 52 , 53 , 54 , 55 , 56 , 57 ], Chryseobacterium [ 58 , 59 ], Fervidobacterium [ 60 , 61 ], Kocuria [ 62 ], Lysobacter [ 63 ], Staphylococcus [ 64 , 65 ], Stenotrophomonas [ 66 , 67 ], Streptomyces [ 68 ], Thermoactinomyces [ 69 , 70 ], Vibrio [ 71 , 72 ], and so on. The ability of microorganisms to hydrolyze keratin is determined by amino groups, the mass loss of the keratin substrate, the amino acid profile, substrate alkalization, the release of ammonia/peptides, and the excretion of sulfate or sulfhydryl groups [ 73 ].…”