Isolation, Identification, and Characterization of the Nematophagous Fungus Arthrobotrys oligospora from Kyrgyzstan

Döölotkeldieva, Tinatin; Bobushova, Saikal; Muratbekova, Ayperi; Schuster, Christina; Leclerque, Andreas

doi:10.1007/s11686-021-00404-5

Cited by 4 publications

(7 citation statements)

References 45 publications

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“…However, this statement requires additional experiments. Based on the results of physiological indicators of tomato plants after 65 days of growth, no reliable data were obtained on the influence of nematodes and predatory fungi on growth processes, which does not contradict the data obtained by other authors [10].…”

Section: Resultscontrasting

confidence: 64%

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The use of predatory fungi of the genus Arthrobotrys isolated from soil in the farmlands of Southern Kazakhstan to control nematode infections in tomato plants

Kanalbek,

Alimbayev,

Ultanbekova

et al. 2024

BIO Web Conf.

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In Kazakhstan, parasitic nematodes affect up to 35-40% of the harvest of sugar beets, potatoes and tomatoes. Until recently, сhemical nematicides were used to control nematode numbers but posed risks of toxicity to humans and animals, and their effectiveness decreased due to resistant nematodes. There is an urgent need to create alternative non-toxic plant protection products. Arthrobotrys oligospora is one of the most studied hyphomycete fungi that is used to create biological products against nematodes. In a laboratory experiment in a pot culture, the activity of Arthrobotrys predatory fungi against parasitic nematodes of the genus Meloidogyne was assessed when growing tomatoes of the «F1 Russian size» variety in a vermiculite/soil mixture. Physiological indicators of plant growth (stem length, number of leaves, stem and root weight) were used for analysis. To ensure the reliability of the obtained results of the activity of predatory fungi, the number of nematodes and the presence of colonies of native predatory fungi in the soil samples of the used variants post-experiment were taken into account. The length of stems, the number of leaves and the weight of roots and stems during the growing season changed within the experimental error. The most significant result was estimated by the number of live nematodes post-experiment. It was shown that when preparations of predatory fungi were added to the soil, the number of nematodes significantly decreased in all variants. Thus, the nematophagous activity of local predatory fungi of the genus Arthrobotrys in tomato cultivation has been proven.

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Section: Resultscontrasting

confidence: 64%

“…Experimental plants were grown indoors at a temperature of 20°C, illumination of 1600 lux, and a 16-hour photoperiod. Predatory fungi and nematodes were added to pots after the formation of full leaves in experimental plants [10].…”

Section: Growing Plantsmentioning

confidence: 99%

The use of predatory fungi of the genus Arthrobotrys isolated from soil in the farmlands of Southern Kazakhstan to control nematode infections in tomato plants

Kanalbek,

Alimbayev,

Ultanbekova

et al. 2024

BIO Web Conf.

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“…Soils, as rich microbial habitats, harbor numerous natural enemies of nematodes, including mites, bacteria, fungi, and viruses [16]. Nematophagous fungi, in particular, are considered natural antagonists that participate in farmland ecosystem food chains as decomposers [17]. In response to the presence of nematodes, these fungi modify their hyphal structures into specialized rings or other forms of traps, transforming into predators that prey on nematodes [18].…”

Section: Introductionmentioning

confidence: 99%

Isolation, Identification, and Evaluation of the Predatory Activity of Chinese Arthrobotrys Species towards Economically Important Plant-Parasitic Nematodes

Wu,

Yang,

Jiang

et al. 2023

JoF

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The current investigation aimed to isolate and identify predatory fungal strains and evaluate their efficacy in mitigating the effects of plant-parasitic nematodes. We successfully isolated three distinct nematophagous fungal strains from soil samples, identified as Arthrobotrys megalosporus, A. oligospora, and A. sinensis, using conventional and molecular identification methodologies. In vitro trials illustrated the high capture efficiency of these fungi against plant-parasitic nematodes. Over an exposure period of 48 h to Aphelenchoides besseyi, Bursaphelenchus xylophilus, and Ditylenchus destructor, A. megalosporus (GUCC220044) displayed predation rates of 99.7%, 83.0%, and 21.1%, respectively. A. oligospora (GUCC220045) demonstrated predation rates of 97.3%, 97.3%, and 54.6%, and A. sinensis (GUCC220046) showed rates of 85.1%, 68.3%, and 19.0% against the same cohort of nematodes. The experimental outcomes substantiate that all three identified fungal strains demonstrate predatory activity against the tested nematodes, albeit with varying efficiencies.

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“…Arthrobotrys oligospora is a nematophagous fungus widely found in the natural habitat. It is currently the most promising fungus used for biological control [1,4]. In response to the environmental stress or nematode stimulation, A. oligospora produces a specific predatory structure, precisely, a sticky web to capture the nematodes.…”

Section: Introductionmentioning

confidence: 99%

“…In response to the environmental stress or nematode stimulation, A. oligospora produces a specific predatory structure, precisely, a sticky web to capture the nematodes. It also secretes extracellular hydrolases and participates in nematode immobilization, cuticle invasion, and nematode cell degradation [4][5][6][7][8]. A. oligospora-secreted extracellular hydrolases mainly include serine proteases and chitinases.…”

Section: Introductionmentioning

confidence: 99%

Biological Characteristics of Recombinant Arthrobotrys oligospora Chitinase AO-801

Gong

Meng²,

Qiao³

et al. 2022

Korean J Parasitol

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Chitinase AO-801 is a hydrolase secreted by Arthrobotrys oligospora during nematode feeding, while its role remained elusive. This study analyzed the molecular characteristics of recombinant chitinase of Arthrobotrys oligospora (reAO-801). AO-801 belongs to the typical glycoside hydrolase 18 family with conserved chitinase sequence and tertiary structure of (α/β)8 triose-phosphate isomerase (TIM) barrel. The molecular weight of reAO-801 was 42 kDa. reAO-801 effectively degraded colloidal and powdered chitin, egg lysate, and stage I larval lysate of Caenorhabditis elegans. The activity of reAO-801 reached its peak at 40˚C and pH values between 4-7. Enzyme activity was inhibited by Zn2+, Ca2+, and Fe3+, whereas Mg2+ and K+ potentiated its activity. In addition, urea, sodium dodecyl sulfate, and 2-mercaptoethanol significantly inhibited enzyme activity. reAO-801 showed complete nematicidal activity against C. elegans stage I larvae. reAO-801 broke down the C. elegans egg shells, causing them to die or die prematurely by hatching the eggs. It also invoked degradation of Haemonchus contortus eggs, resulting in apparent changes in the morphological structure. This study demonstrated the cytotoxic effect of reAO-801, which laid the foundation for further dissecting the mechanism of nematode infestation by A. oligospora.

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Isolation, Identification, and Characterization of the Nematophagous Fungus Arthrobotrys oligospora from Kyrgyzstan

Cited by 4 publications

References 45 publications

The use of predatory fungi of the genus Arthrobotrys isolated from soil in the farmlands of Southern Kazakhstan to control nematode infections in tomato plants

The use of predatory fungi of the genus Arthrobotrys isolated from soil in the farmlands of Southern Kazakhstan to control nematode infections in tomato plants

Isolation, Identification, and Evaluation of the Predatory Activity of Chinese Arthrobotrys Species towards Economically Important Plant-Parasitic Nematodes

Biological Characteristics of Recombinant Arthrobotrys oligospora Chitinase AO-801

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