Isolation, Identification and Characterization of a New Type of Lectin with α-Amylase Inhibitory Activity in Chickpea (Cicer arietinum L.)

Wang, Zhankui; Chen, Ming; Zhu, Yajing; Qian, Peipei; Zhou, Y. M.; Ji, Wei; Shen, Yingzi; Mijiti, Abudoukeyumu; Gu, Aiqin; Wang, Zeng; Zhang, Hua; Ma, Hao

doi:10.2174/0929866524666170711120501

Cited by 5 publications

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“…Previous reports of chickpea lectin on various facets include characterization of a clone encoding vegetative lectin from Cicer arietinum epicotyls (Esteban et al 2002), production of stable transgene chickpea through expression of insecticidal lectin (Chakraborti et al 2009) and root lectins (Agrawal et al 2011). In addition, in silico studies on recombinant chickpea lectin (Wakankar et al 2013), three dimensional structure determination (Sharma et al 2015) and a-amylase inhibitory activity against potato beetle larvae (Wang et al 2017) are also available in literature domain.…”

Section: Introductionmentioning

confidence: 99%

Characterization of chickpea (Cicer arietinum L.) lectin for biological activity

Gautam

Gupta

Narvekar

et al. 2018

Physiol Mol Biol Plants

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Lectins are proteins that are subject of intense investigations. Information on lectin from chickpea ( L.) with respect to its biological activities are very limited. In this study, we purified lectin from the seeds of chickpea employing DEAE-cellulose and SP-Sephadex ion exchange chromatography and identified its molecular subunit mass as 35 kDa. The free radical scavenging activity of lectin measured by the DPPH assay has IC of 0.88 µg/mL. Lectin exerted antifungal activity against ,, and, while antibacterial activity against ,, and The minimum inhibitory concentrations were 200, 240, 160 and 140 µg for , and respectively. Lectin was further examined for its antiproliferative potential against cancerous cell line. The cell viability assay indicated a high inhibition activity on Ishikawa, HepG2, MCF-7 and MDA-MB-231 with IC value of 46.67, 44.20, 53.58 and 37.46 µg/mL respectively. These results can provide a background for future research into the benefits of chickpea lectin to pharmacological perspective.

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Section: Introductionmentioning

confidence: 99%

Characterization of chickpea (Cicer arietinum L.) lectin for biological activity

Gautam

Gupta

Narvekar

et al. 2018

Physiol Mol Biol Plants

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“…It should be noted that CAL is not part of the lectin-arcelin-aAI1 supergene family. The IC 50 value was not indicated [ 39 ].…”

Section: Peptides Against Obesity and Insulin Resistancementioning

confidence: 99%

Bioactive Peptides Obtained from Legume Seeds as New Compounds in Metabolic Syndrome Prevention and Diet Therapy

Kiersnowska

Jakubczyk

2022

Foods

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Currently, food is regarded not only as a source of nutrients, vitamins, and minerals but also as a source of bioactive compounds that can play a significant role in the prevention and diet therapy of many diseases. Metabolic syndrome (MS) is a complex disorder defined as a set of interrelated factors that increase the risk of cardiovascular disease, atherosclerosis, type 2 diabetes, or dyslipidemia. MS affects not only adults but also children. Peptides are one of the compounds that exhibit a variety of bioactive properties. They are derived from food proteins, which are usually obtained through enzymatic hydrolysis or digestion in the digestive system. Legume seeds are a good source of bioactive peptides. In addition to their high protein content, they contain high levels of dietary fiber, vitamins, and minerals. The aim of this review is to present new bioactive peptides derived from legume seeds and showing inhibitory properties against MS. These compounds may find application in MS diet therapy or functional food production.

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“…Chickpea possesses high-quality starch, protein, fat and dietary fiber ( 3 ), and plays an important role in human diets. Previous studies have shown that chickpea possesses various biological activities, such as antioxidant ( 4 ), anti-inflammatory ( 5 ), amylase inhibitor ( 6 ), and angiotensin I-converting enzyme (ACE) inhibitory ( 7 ) activities.…”

Section: Introductionmentioning

confidence: 99%

Structural characterization and bioactivity evaluation of water-extractable polysaccharides from chickpeas (Cicer arietinum L.) seeds

Zhu

Dun²,

Shi³

et al. 2022

Front. Nutr.

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Two water-extractable polysaccharide fractions designated as CWP (7. 37 × 105 Da) and CWP-0.2 (1.58 × 104 Da) were isolated and purified from chickpea (Cicer arietinum L.) seeds. The chemical structure of the two polysaccharides was characterized by various methods. Monosaccharide composition and methylation analysis showed that CWP was composed of Man and Glc in a molar ratio of 44.6:55.4, and CWP-0.2 was composed of Rha, Ara, Man, Glc, and Gal in a molar ratio of 10.6:23.3:5.2:4.9:56. Further structural characterization indicated that the main chain connection of CWP was → (2-β-d-Fruf-1) n →, and the main chain connection of CWP-0.2 was explored as → 2,4)-α-l-Rhap-(1 → 3)-α-d-Galp-(1 → with the branched chain of → 2,4)-α-l-Rhap-(1 → o-4. Besides, both CWP and CWP-0.2 had antioxidant and immunoregulatory activity in vitro, through scavenging DPPH· and ABTS·+ as well as stimulating production of NO, IL-6, TNF-α and MCP-1 in RAW 264.7 macrophages. CWP-0.2 revealed significantly higher bioactivity than CWP.

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Isolation, Identification and Characterization of a New Type of Lectin with α-Amylase Inhibitory Activity in Chickpea (Cicer arietinum L.)

Cited by 5 publications

References 0 publications

Characterization of chickpea (Cicer arietinum L.) lectin for biological activity

Characterization of chickpea (Cicer arietinum L.) lectin for biological activity

Bioactive Peptides Obtained from Legume Seeds as New Compounds in Metabolic Syndrome Prevention and Diet Therapy

Structural characterization and bioactivity evaluation of water-extractable polysaccharides from chickpeas (Cicer arietinum L.) seeds

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