Isolation, identification and characterization of Bacillus amyloliquefaciens BZ-6, a bacterial isolate for enhancing oil recovery from oily sludge

Liu, Wuxing; Wang, Xiaobing; Wu, L. P.; Chen, Mengfang; Tu, Chen‐Pei D.; Luo, Yongming; Christie, Peter

doi:10.1016/j.chemosphere.2012.01.059

Cited by 42 publications

(13 citation statements)

References 21 publications

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“…Water soluble carbon sources used in the present study are commonly employed for biosurfactant production [6,12]. However, there is no detailed description of physiochemical and biological properties of lipopeptide produced in the presence of different carbon source.…”

Section: Discussionmentioning

confidence: 99%

“…Liu et al (2013) reported sucrose as the best carbon source among the water soluble carbon sources like glucose, galactose, maltose, glycerol, mannitol, soluble starch and dextrin for biosurfactant production [12]. Presence of array of glycoside hydrolases in B. amyloliquefaciens species bestows these microorganisms to utilize diverse range of carbohydrate [14].…”

Section: Discussionmentioning

confidence: 99%

“…Presence of array of glycoside hydrolases in B. amyloliquefaciens species bestows these microorganisms to utilize diverse range of carbohydrate [14]. Several studies have shown that in Bacillus species biosurfactant production is directly correlated to the biomass production [6,10,12]. Generally substrate supporting good growth also supports maximum biosurfactant production.…”

Section: Discussionmentioning

confidence: 99%

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Substrate dependent in vitro antifungal activity of Bacillus sp strain AR2

2014

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BackgroundBiosurfactants are a structurally diverse group of secondary metabolites with lots of potential to serve mankind. Depending upon the structure and composition they may exhibit properties that make them suitable for a particular application. Structural and compositional diversity of biosurfactant is unambiguously substrate dependent. The present study investigates the qualitative and quantitative effect of different water soluble carbon source on the biosurfactant produced by Bacillus amylofaciens strain AR2.ResultsStrain AR2 produced lipopeptide type biosurfactant while growing on water soluble carbon sources. Maximum biosurfactant production was observed in the sucrose supplemented minimal salt medium (MSM). Strain AR2 exhibited carbon source dependent surface tension reduction in the range of 30-37 mN/m, critical micelle concentration (CMC) in the range 80-110 mg/l and emulsification index (EI24 kerosene) in the range of 32-66%. In dextrose, sucrose and glycerol supplemented MSM, strain AR2 produced lipopeptides as a mixture of surfactin, iturin and fengycin. However, in the presence of maltose, lactose and sorbitol only iturin was produced. This substrate dependent compositional variation in the lipopeptides significantly influenced antifungal activity. Lipopeptides produced by strain AR2 while growing on sucrose and dextrose based MSM was observed to be most efficient as an antifungal agent.ConclusionsThese results suggest that carbon source provided for the growth and biosurfactant production not only influences the yield but also the type of biosurfactant. Sucrose is the most suitable carbon source for production of lipopeptide biosurfactant with antifungal activity.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Substrate dependent in vitro antifungal activity of Bacillus sp strain AR2

2014

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“…They can also be produced from renewable and cheaper substrates than many synthetic surfactants (Banat et al 2000). A number of studies have been successfully carried out on soil bioremediation using biosurfactants (Cameotra and Singh 2008;Lai et al 2009;Liu et al 2012;Urum et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Rhizobacteria (Pseudomonas sp. SB) assist phytoremediation of oily-sludge-contaminated soil by tall fescue (Testuca arundinacea L.)

et al. 2013

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Background and aims The objectives of this study were to examine the effect of direct inoculation of seeds with the rhizobacteria Pseudomonas sp. SB on the growth of tall fescue and phytodegradation efficiency in an oily-sludge-contaminated soil. Methods SB isolated from rhizosphere soil of tall fescue was evaluated for their plant-growth-promoting characters and ability to produce biosurfactant. A pot experiment was conducted to study the effect of inoculation of SB on phytoremediation.Results SB reduced the surface tension of culture media and produced indole acetic acid, siderophores, and 1-aminocyclopropane-1-carboxylate deaminase. Inoculation of SB increased shoot and root dry weights of tall fescue in oily-sludge-contaminated soil by 28 % and 19 %, respectively. Over 120 days, the content of total petroleum hydrocarbon in soil decreased by 33.9 %, 68.0 %, and 84.5 %, and of polycyclic aromatic hydrocarbons (PAHs) by 32.9 %, 40.9 %, and 46.2 %, respectively, in the no-plant control, tall fescue, and tall fescue + SB treatments. Inoculation of SB also increased the activity and biodiversity of soil microbial communities in the planted treatments. Conclusions SB could produce biosurfactant and exhibited a number of characters of plant-growthpromoting rhizobacteria. Inoculation of SB to tall fescue led to more effective remediation of oilysludge-contaminated soils.

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“…by mass, but more frequently in the range of 15-50% (Tahhan et al, 2011;Biswal et al, 2009;Mohan and Chandrasekhar, 2011;Liu et al, 2010;Liu et al, 2012), whereas the contents of water and solids are in the range of 30-85% and 5-46%, respectively (Ramaswamy et al, 2007). The PHCs and other organic compounds in oily sludge can be generally classified into four fractions, including aliphatics, aromatics, nitrogen sulphur oxygen (NSO) containing compounds, and asphaltenes (Mrayyan and Battikhi, 2005;Reddy et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Development of novel oil recovery methods for petroleum refinery oily sludge treatment

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Oily sludge is one of the most significant wastes generated in the petroleum industry.It is a complex emulsion of various petroleum hydrocarbons (PHCs), water, metals, and fine solids. Due to its hazardous nature and increased generation quantities around the world, the effective treatment of oily sludge has attracted widespread attention. The complexity of its composition and diversity of its origin sources make oily sludge management a difficult and costly undertaking. Many methods have been developed for the treatment of oily sludge through oil recovery or sludge disposal approaches, but no single specific process can be considered as a panacea since each method is associated with different advantages and limitations. Efforts should focus on the improvement of current technologies and the combination of oil recovery with sludge disposal in order to comply with both resource reuse recommendations and environmental regulations. The object of this study was to develop novel combined methods for oil recovery treatment on different refinery oily sludges. The investigation focused on the oil recovery performance of combined methods based on four individual treatment processes including ultrasonic irradiation, solvent extraction, freeze/thaw, and pyrolysis in oily sludge treatment.Firstly, the oil recovery and salt removal effects of ultrasonic irradiation on oil refinery tank bottom sludge were investigated, together with those of direct heating.Ultrasonic power, treatment duration, sludge-to-water ratio, and initial sludge-water slurry temperature were examined for their impacts on sludge treatment. It was found that the increased initial slurry temperature could enhance the ultrasonic irradiation performance, especially at lower ultrasonic power level (i.e., 21 W), but the application of higher-power ultrasonic irradiation could rapidly increase the bulk temperature of slurry. Ultrasonic 2 irradiation had a better oil recovery and salt removal performance than direct heating treatment. More than 60% of PH Cs in the sludge was recovered at an ultrasonic power of 75 W, a treatment duration of 6 min, an initial slurry temperature of 25 °C, and a sludgeto-water ratio of 1 :4, while salt content in the recovered oil was reduced to <5 mg/L, thereby satisfying the salt requirement in refinery feedstock oil. In general, ultrasonic irradiation could be an effective method in terms of oil recovery and salt removal from refinery oily sludge, but the separated wastewater still contains relatively high concentrations of PH Cs and salt which requires proper treatment.Two types of ultrasonic assisted extraction (UAE) treatment including UAE probe (UAEP) system and UAE bath (UAEB) system were investigated for oil recovery from refinery oily sludge. Their oil recovery efficiencies were compared to that of mechanical shaking extraction (MSE). Three solvents including cyclohexane (CHX), ethyl acetate (EA), and methyl ethyl ketone (MEK) were examined as the extraction solvents. The influence of experimental factors on oil an...

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Isolation, identification and characterization of Bacillus amyloliquefaciens BZ-6, a bacterial isolate for enhancing oil recovery from oily sludge

Cited by 42 publications

References 21 publications

Substrate dependent in vitro antifungal activity of Bacillus sp strain AR2

Substrate dependent in vitro antifungal activity of Bacillus sp strain AR2

Rhizobacteria (Pseudomonas sp. SB) assist phytoremediation of oily-sludge-contaminated soil by tall fescue (Testuca arundinacea L.)

Development of novel oil recovery methods for petroleum refinery oily sludge treatment

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