Isolation, Identification, and Characterization of a Novel, Oil-Degrading Bacterium, Pseudomonas aeruginosa T1

Hasanuzzaman, Mohammad; Umadhay-Briones, Kathryn M; Zsíros, Szilvia; Morita, Naoki; Nodasaka, Yoshinobu; Yumoto, Isao; Okuyama, Hidetoshi

doi:10.1007/s00284-004-4267-x

Cited by 46 publications

(29 citation statements)

References 29 publications

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“…The samples were transferred to the laboratory and were spread on Luria-Bertani (LB) agar (1% NaCl, 1% tryptone, 0.5% yeast extract and 1.5% agar) plates and incubated at 37°C for 24 h on the same day. Samples were enriched using the method of Hasanuzzaman et al (2004) …”

Section: Samples Collection and Enrichmentmentioning

confidence: 99%

Characterization of Lipase from Bacillus subtilisI-4 and Its Potential Use in Oil Contaminated Wastewater

Iqbal

Rehman

2015

Braz. arch. biol. technol.

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Section: Samples Collection and Enrichmentmentioning

confidence: 99%

Characterization of Lipase from Bacillus subtilisI-4 and Its Potential Use in Oil Contaminated Wastewater

Iqbal

Rehman

2015

Braz. arch. biol. technol.

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“…Pseudomonads are effective degraders capable of utilizing a wide range of simple and complex organic compounds (Hasanuzzaman et al, 2004). However, no report on degradation of methylindoles by Pseudomonas aeruginosa is available.…”

Section: Characterization Of Indolic Compounds Degrading Bacteriamentioning

confidence: 99%

Biodegradation of 1-Methylindole and 3-Methylindole by Mangrove Sediment Enrichment Cultures and a Pure Culture of an Isolated Pseudomonas Aeruginosa Gs

Yin

Huang

2006

Water Air Soil Pollut

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Abstract. Indolic compounds are N-heterocyclic aromatic chemicals and have been detected at contaminated sites. Biodegradation of 1-methylindole (1MI) and 3-methylindole (3MI) was investigated initially using enrichment cultures with mangrove sediment obtained from Mai Po Nature Reserve of Hong Kong and subsequently with a pure culture of Pseudomonas aeruginosa Gs confirmed with 16S rRNA gene. At 2.0 mM, 1MI and 3MI were degraded in 4 and 3 days, respectively, by the respective 1MI-and 3MI-degrading enrichment cultures. When substrate concentrations were increased to 3.0 mM and 3.5 mM, slower degradation of 1MI and 3MI was observed indicating inhibitory effects from the substrates, possibly due to toxicity. In addition, no colony of bacteria could be observed on the agar plates amended with 3.5 mM 1MI or 4.0 mM 3MI, indicating that 1MI was more toxic than 3MI. Pseudomonas aeruginosa Gs, isolated from the enrichment culture, effectively utilized both substrates as the sole source of carbon and energy. Complete degradation of 1MI and 3MI was achieved after more than 40 days and 24 days, respectively, at an initial concentration of 2 mM in the culture. Effects of initial substrate concentration, pH and salinity on degradation of 1MI and 3MI by P. aeruginosa Gs were also studied in batch culture. The optimum pH and salinity for degrading both substrates by P. aeruginosa Gs was 7.0 and 5‰, respectively. Biodegradation kinetics of 1MI and 3MI by P. aeruginosa Gs could be described using a first-order kinetic model. Our results suggest that both 1MI and 3MI are biodegradable in the mangrove environment and that toxicity of 1MI could be a potential factor limiting the removal of the chemical in the environment by microorganisms.

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“…Other studies indicated that, pathogenic bacteria such as P. aeruginosa able to utilize crude oil can be isolated from the environment (Chaerun et al, [11]; Hasanuzzaman et al, [12]). However, none of the medical strains grew in the presence of crude oil.…”

Section: Resultsmentioning

confidence: 99%

Occurrence of Pseudomonas aeruginosa in Kuwait environment

Akbar¹,

AL-Otaibi²,

Drobiova³

et al. 2008

WIT Transactions on the Built Environment

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Deliberate and accidental releases of opportunistic pathogens such as Pseudomonas aeruginosa into the environment increase the likelihood of spreading infectious diseases. Also, P. aeruginosa is responsible for nosocomial infections and usually harbour several antibiotic resistance genes. In the present study total of 76 strains of P. aeruginosa were characterized (54 strains were isolated from soil samples collected from a crude oil contaminated site located north of Kuwait and 22 strains were isolated from medical samples). Whereas the phenotypic characterization such as the biochemical tests using VITEK 2 system (bioMérieux) and antibiotics susceptibility testing (AST) showed the high similarity between the medical and the environmental strains, the molecular analyses based on multilocus sequence typing (MLST) and restriction fragment length polymorphism (RFLP) demonstrated the heterogeneity of isolated P. aeruginosa indicating the presence of different clusters. Comparison of fingerprints obtained, confirmed the negative correlation between the environmental and the medical strains. In addition, results demonstrated that environmental strains are better adapted to crude oil contaminated sites. However, environmental strains may represent hidden reservoirs for pathogenic traits that may disseminate to other bacteria in the environment.

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Isolation, Identification, and Characterization of a Novel, Oil-Degrading Bacterium, Pseudomonas aeruginosa T1

Cited by 46 publications

References 29 publications

Characterization of Lipase from Bacillus subtilisI-4 and Its Potential Use in Oil Contaminated Wastewater

Characterization of Lipase from Bacillus subtilisI-4 and Its Potential Use in Oil Contaminated Wastewater

Biodegradation of 1-Methylindole and 3-Methylindole by Mangrove Sediment Enrichment Cultures and a Pure Culture of an Isolated Pseudomonas Aeruginosa Gs

Occurrence of Pseudomonas aeruginosa in Kuwait environment

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