Isolation, Identification and Characterization of Paenibacillus pabuli E1 to Explore Its Aflatoxin B1 Degradation Potential

Li, Gen; Li, Xiaoyu; Dong, Liming; Li, Caixia; Zou, Panpan; Saleemi, M. Kashif; Murtaza, Bilal; Jin, Bowen; Hong, Zhao; Wang, Lili; Li, Shuying; Yang, Huan; Xu, Yongping

doi:10.1007/s00284-021-02624-4

Cited by 12 publications

(4 citation statements)

References 29 publications

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“…The GenBank accession number for P. pabuli E1 is MT322455, and the strain preservation number is CGMCC NO.20517 ( Li et al, 2021 ). All protein-encoding ORFs from P. pabuli E1 genomes were subjected to CAZy annotation using a two-step procedure of annotation and identification.…”

Section: Methodsmentioning

confidence: 99%

Feasibility insights into the application of Paenibacillus pabuli E1 in animal feed to eliminate non-starch polysaccharides

Li,

Yuan,

Jin

et al. 2023

Front. Microbiol.

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The goal of the research was to find alternative protein sources for animal farming that are efficient and cost-effective. The researchers focused on distillers dried grains with solubles (DDGS), a co-product of bioethanol production that is rich in protein but limited in its use as a feed ingredient due to its high non-starch polysaccharides (NSPs) content, particularly for monogastric animals. The analysis of the Paenibacillus pabuli E1 genome revealed the presence of 372 genes related to Carbohydrate-Active enzymes (CAZymes), with 98 of them associated with NSPs degrading enzymes that target cellulose, hemicellulose, and pectin. Additionally, although lignin is not an NSP, two lignin-degrading enzymes were also examined because the presence of lignin alongside NSPs can hinder the catalytic effect of enzymes on NSPs. To confirm the catalytic ability of the degrading enzymes, an in vitro enzyme activity assay was conducted. The results demonstrated that the endoglucanase activity reached 5.37 U/mL, while beta-glucosidase activity was 4.60 U/mL. The filter paper experiments did not detect any reducing sugars. The xylanase and beta-xylosidase activities were measured at 11.05 and 4.16 U/mL, respectively. Furthermore, the pectate lyase and pectin lyase activities were found to be 8.19 and 2.43 U/mL, respectively. The activities of laccase and MnP were determined as 1.87 and 4.30 U/mL, respectively. The researchers also investigated the effect of P. pabuli E1 on the degradation of NSPs through the solid-state fermentation of DDGS. After 240 h of fermentation, the results showed degradation rates of 11.86% for hemicellulose, 11.53% for cellulose, and 8.78% for lignin. Moreover, the crude protein (CP) content of DDGS increased from 26.59% to 30.59%. In conclusion, this study demonstrated that P. pabuli E1 possesses various potential NSPs degrading enzymes that can effectively eliminate NSPs in feed. This process improves the quality and availability of the feed, which is important for animal farming as it seeks alternative protein sources to replace traditional nutrients.

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Section: Methodsmentioning

confidence: 99%

Feasibility insights into the application of Paenibacillus pabuli E1 in animal feed to eliminate non-starch polysaccharides

Li,

Yuan,

Jin

et al. 2023

Front. Microbiol.

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“…Numerous studies have demonstrated that various Fusarium species can produce identical mycotoxins, and conversely, the same Fusarium species can yield multiple mycotoxins (Li et al., 2021). Both humans and animals may encounter a diverse array of mycotoxins through contaminated food or feed (Agriopoulou et al., 2020; Murtaza et al., 2023).…”

Section: Current Status Of Mycotoxin Joint Pollutionmentioning

confidence: 99%

Toxicodynamic of combined mycotoxins: MicroRNAs and acute‐phase proteins as diagnostic biomarkers

Murtaza,

Li,

Nawaz

et al. 2024

Comp Rev Food Sci Food Safe

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Mycotoxins, ubiquitous contaminants in food, present a global threat to human health and well‐being. Mitigation efforts, such as the implementation of sound agricultural practices, thorough food processing, and the advancement of mycotoxin control technologies, have been instrumental in reducing mycotoxin exposure and associated toxicity. To comprehensively assess mycotoxins and their toxicodynamic implications, the deployment of effective and predictive strategies is imperative. Understanding the manner of action, transformation, and cumulative toxic effects of mycotoxins, moreover, their interactions with food matrices can be gleaned through gene expression and transcriptome analyses at cellular and molecular levels. MicroRNAs (miRNAs) govern the expression of target genes and enzymes that play pivotal roles in physiological, pathological, and toxicological responses, whereas acute phase proteins (APPs) exert regulatory control over the metabolism of therapeutic agents, both endogenously and posttranscriptionally. Consequently, this review aims to consolidate current knowledge concerning the regulatory role of miRNAs in the initiation of toxicological pathways by mycotoxins and explores the potential of APPs as biomarkers following mycotoxin exposure. The findings of this research highlight the potential utility of miRNAs and APPs as indicators for the detection and management of mycotoxins in food through biological processes. These markers offer promising avenues for enhancing the safety and quality of food products.

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“…Due to the fact that the husks in distiller's grains can easily puncture the digestive tract of animals, have poor palatability, high acidity, and require the addition of baking soda during the feeding process, 2616-5880 Vol. the proportion of unfermented distiller's grains in the diet cannot exceed 75% [46][47][48][49][50][51][52][53] . Research has found that enzymes produced by microorganisms can degrade cellulose and improve the problem of high crude fiber content in distiller's grains, which is more conducive to animal digestion and improves the palatability of direct feeding of distiller's grains [47] .…”

Section: Using Distiller's Grains To Produce Feedmentioning

confidence: 99%

“…If the protein in the distiller's grains can be extracted first, or further fermented, the protein can be extracted as feed protein [9] , rice husks can be used as fuel to produce heat or cogeneration, and rice husk ash can be used as raw material for organic fertilizer production [12] . In this way, the added value of protein is high, and higher value active proteins can be separated from the protein, which will greatly increase the value of distiller's grains utilization; Low value-added fiber substances, on the other hand, not only achieve resource utilization but also greatly reduce the waste of distiller's grains through combustion and energy utilization; Finally, utilizing distiller's grains ash to produce organic fertilizer [46] can achieve complete harmless treatment of distiller's grains. In the research on the utilization of distiller's grains, most reuse methods require fermentation.…”

Section: Potential Applications and Future Directionsmentioning

confidence: 99%

Research progress in the resource reuse of distiller's grains

2023

AJMC

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Distiller's grains are the main by-products produced in the process of brewing and ethanol production. Although they are rich in nutrients, they are highly susceptible to spoilage. Their unscientific utilization not only causes environmental pollution, but also leads to huge waste of resources. Therefore, it is urgent to carry out research on the resource utilization of distiller's grains. In recent years, various studies have been conducted on the resource utilization of distiller's grains both domestically and internationally. According to the utilization methods, it can be mainly divided into four categories: energy production, agricultural products, food, and chemical raw materials, including the production of biogas, animal feed, flour products, and succinic acid, to achieve the resource utilization of distiller's grains. This article reviews the nutritional composition, production and utilization status of distiller's grains, as well as the challenges and future development directions faced by the resource utilization of distiller's grains, in order to provide reference for the treatment and technical research of distiller's grains.

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Isolation, Identification and Characterization of Paenibacillus pabuli E1 to Explore Its Aflatoxin B1 Degradation Potential

Cited by 12 publications

References 29 publications

Feasibility insights into the application of Paenibacillus pabuli E1 in animal feed to eliminate non-starch polysaccharides

Feasibility insights into the application of Paenibacillus pabuli E1 in animal feed to eliminate non-starch polysaccharides

Toxicodynamic of combined mycotoxins: MicroRNAs and acute‐phase proteins as diagnostic biomarkers

Research progress in the resource reuse of distiller's grains

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