Isolation, identification and characterization of a novel antimicrobial peptide from Moringa oleifera seeds based on affinity adsorption

Wang, Xuefeng; He, Li; Huang, Zhiyuan; Zhao, Qiong; Fan, Jie; Yang, Tian; Huang, Aixiang

doi:10.1016/j.foodchem.2022.133923

Cited by 26 publications

(6 citation statements)

References 38 publications

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“…EYHp6 contained 12 amino acids with 45% hydrophobicity and a net charge of −1. The MIC value of EYHp6 was comparable to those of anionic antimicrobial peptides AP1 (GEQGALAQFGEWL) and MOp3 (MCNDCGA) [28,29] and cationic antimicrobial peptide BCp12 (YLGYLEQLLRLK) [30], which ranged from 1 to 4.4 mM. Peptides derived from protein hydrolysate usually exhibit MIC values in mM levels, which are less potent than those derived from natural sources whose MIC values typically range in µM levels [21,31,32].…”

Section: Antimicrobial Activitymentioning

confidence: 90%

Anti-Salmonella Activity of a Novel Peptide, KGGDLGLFEPTL, Derived from Egg Yolk Hydrolysate

Pimchan,

Tian,

Thumanu

et al. 2023

Antibiotics

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The present study aimed to characterize the mode of action of a novel antimicrobial peptide isolated from egg yolk hydrolysate. The EYHp6, KGGDLGLFEPTL, exhibited inhibition against Salmonella enterica serovar Typhimurium TISTR 292 and S. enterica serovar Enteritidis DMST 15679 with a MIC value of 2 mM. In contrast, S. enterica serovar Newport ATCC 6962 and other strains of Typhimurium and Enteritidis were inhibited at 4 mM. EYHp6 increased the cell membrane permeability of S. Typhimurium TISTR 292, leading to DNA leakage. Membrane integrity determined by propidium iodide and SYTO9 staining visualized by confocal microscopy demonstrated that EYHp6 at 1 × MIC induced disruption of cell membranes. Electron microscopy revealed that treatment of S. Typhimurium with EYHp6 led to damage to the cell membrane, causing the leakage of intracellular contents. Synchrotron-based Fourier-transform infrared spectroscopy indicated that EYHp6 killed S. Typhimurium by targeting fatty acids and nucleic acids in the cell membrane. The peptide did not show hemolytic activity up to 4 mM. These findings suggest that EYHp6 could be a promising antibacterial agent for controlling the growth of S. enterica.

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Section: Antimicrobial Activitymentioning

confidence: 90%

Anti-Salmonella Activity of a Novel Peptide, KGGDLGLFEPTL, Derived from Egg Yolk Hydrolysate

Pimchan,

Tian,

Thumanu

et al. 2023

Antibiotics

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“…Another peptide (<7.5 kDa) has been isolated from MO and possessed an efcient antimicrobial activity [216]. An antimicrobial peptide (AMP) has been also, isolated from MO seeds with an inhibitory action against S. aureus through inhibition of DNA gyrase and dihydrofolate reductase [96].…”

Section: Moringa Oleifera and Proteinsmentioning

confidence: 99%

Moringa oleifera: Recent Insights for Its Biochemical and Medicinal Applications

Abdelazim,

Afifi,

Abu-Alghayth

et al. 2024

Journal of Food Biochemistry

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Plants could be used for multiple medicinal purposes. Moringa oleifera (MO) is considered the most famous plant used for this purpose. The present review aimed to spot the light on the recent medicinal, biochemical, and nutritional applications of MO. The plant contains a huge number of nutrients such as fatty acids, amino acids, proteins, polysaccharides, minerals, and vitamins. It has been used to control glucose, lipids, proteins, minerals, vitamins, hormones, and antioxidants in many animals and human models. Its medicinal applications are also varied and wide; it could be used to control and manage lots of disorders. Extracts and isolated ingredients from the plant open the way for many researchers all over the world to study its biochemical and medicinal impact on many experimental and clinical models. The present review spots the light on the recent medicinal and biochemical significance of MO opening the discussion and demonstrating the strengths and weaknesses in the MO research area. Regardless of the contrary concept, we considered MO a promising plant that could be studied for its medicinal applications on both biochemical and molecular levels. We recommended further research on the molecular effects of MO in healthy and diseased models.

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“…To date, fungal cell factories for efficient and sustainable production of proteins and peptides endowed with technological and healthy properties represent a current challenge [ 86 ]. Likewise, edible fungi, M. oleifera seed or leaves protein hydrolysates and related purified peptides showed several beneficial effects, including antioxidant and anti-inflammatory [ 255 ], hypoglycemic [ 231 ], antimicrobial [ 232 ], antihypertensive [ 229 ] properties (see Table 3 ). Partially purified peptides fractions suitable for the regulation of hyperuricemia were also obtained from tryptic hydrolysate of M. oleifera leaves.…”

Section: Bioactive Properties Of Non-meat Proteinsmentioning

confidence: 99%

Alternative Protein Sources and Novel Foods: Benefits, Food Applications and Safety Issues

et al. 2023

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The increasing size of the human population and the shortage of highly valuable proteinaceous ingredients has prompted the international community to scout for new, sustainable, and natural protein resources from invertebrates (e.g., insects) and underutilized legume crops, unexploited terrestrial and aquatic weeds, and fungi. Insect proteins are known for their nutritional value, being rich in proteins with a good balance of essential amino acids and being a valuable source of essential fatty acids and trace elements. Unconventional legume crops were found rich in nutritional, phytochemical, and therapeutic properties, showing excellent abilities to survive extreme environmental conditions. This review evaluates the recent state of underutilized legume crops, aquatic weeds, fungi, and insects intended as alternative protein sources, from ingredient production to their incorporation in food products, including their food formulations and the functional characteristics of alternative plant-based proteins and edible insect proteins as novel foods. Emphasis is also placed on safety issues due to the presence of anti-nutritional factors and allergenic proteins in insects and/or underutilized legumes. The functional and biological activities of protein hydrolysates from different protein sources are reviewed, along with bioactive peptides displaying antihypertensive, antioxidant, antidiabetic, and/or antimicrobial activity. Due to the healthy properties of these foods for the high abundance of bioactive peptides and phytochemicals, more consumers are expected to turn to vegetarianism or veganism in the future, and the increasing demand for such products will be a challenge for the future.

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Isolation, identification and characterization of a novel antimicrobial peptide from Moringa oleifera seeds based on affinity adsorption

Cited by 26 publications

References 38 publications

Anti-Salmonella Activity of a Novel Peptide, KGGDLGLFEPTL, Derived from Egg Yolk Hydrolysate

Anti-Salmonella Activity of a Novel Peptide, KGGDLGLFEPTL, Derived from Egg Yolk Hydrolysate

Moringa oleifera: Recent Insights for Its Biochemical and Medicinal Applications

Alternative Protein Sources and Novel Foods: Benefits, Food Applications and Safety Issues

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