Isolation, Identification, and Characterization of Aeromonas veronii from Chinese Soft-Shelled Turtle (Trionyx sinensis)

Hu, Xiaowei; Xiao, Zidong; Li, Bo; Xue, Mingyang; Jiang, Nan; Fan, Yuding; Chen, Peng; Feng, Qibo; Kong, Xianghui; Zhou, Yong

doi:10.3390/microorganisms11051304

Cited by 11 publications

(6 citation statements)

References 32 publications

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“…eBURST analysis revealed the formation of one clonal complex (ST1027, ST1047), and the observed high nucleotide diversity suggested that most strains possessed unique sequence types, indicating a high level of genetic diversity. It is important to note that although MLST can provide valuable insights into the clonal relationships among bacterial strains, it may only capture part of the complexity of their evolutionary history [13,67]. Finally, recombination event analysis using RDP5 and Clonal-FrameML software detected 42 and 49 recombination events, respectively, with 22 results validated by four or more algorithms.…”

Section: Plos Onementioning

confidence: 99%

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Multilocus sequence typing and antibiotic resistance of Aeromonas isolated from freshwater fish in Hebei Province

Yu,

Oh,

Kim

et al. 2024

PLoS ONE

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Aeromonas spp. are the opportunistic pathogens that infect both aquatic and terrestrial homeotherms. They were commonly present in aquatic environments, including effluent, tap water, marine, river, and lake, where they are often isolated from aquatic animals, including fish, molluscs, and crustaceans. The Aeromonas infections can cause sepsis, ulcer, and other symptoms, resulting in the death of massive aquatic animals. Therefore, the prevention and control of Aeromonas is of great significance for the healthy development of aquaculture. In this study, we used modern molecular methods to enhance disease control of Aeromonas isolates from freshwater fish in Hebei Province. A total of 130 Aeromonas spp. isolates were isolated from freshwater fish farms in Hengshui, Handan, and Shijiazhuang and all 130 Aeromonas spp. isolates were sequenced for species identification. Of the 130 Aeromonas spp. isolates, 104 isolates were successfully sequenced, and BLAST analysis showed that Aeromonas veronii was predominant in freshwater fish farms in Hebei Province. In addition, 26 antibiotic resistance profiles were obtained from 102 fully cultured isolates among the 104 Aeromonas spp. isolates whose species was primarily identified, and 44 multidrug-resistant bacteria among the 102 isolates were identified using an antibiotic susceptibility test. Using the Multilocus Sequence Typing (MLST) method, 33 out of 44 multidrug-resistant isolates with 14 non-Aeromonas reference strains were selected for phylogenetic and MLST analysis, and all 33 multidrug-resistant isolates were A. veronii. A total of 30 new Sequence Types (STs) were obtained by comparing concatenated sequences (gyrB-groL-gltA-metG-ppsA-recA) on PubMLST website. Furthermore, recombination event analysis detected using RDP5 and ClonalFrameML software 42 and 49 recombination events, respectively, and 22 recombination events were validated by four or more algorithms. Since mutation and recombination events increase clonal diversity and single housekeeping gene sequence alignments are limited for identifying species, we propose the use of multiple concatenated sequence loci to increase discriminatory power. In addition, we propose that the MLST method is an appropriate technique to study and develop the resistance mechanisms of multidrug-resistant Aeromonas and to identify Aeromonas systematically in complex samples obtained from the environment.

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Section: Plos Onementioning

confidence: 99%

“…have increased, including A. hydrophila, A. sobria, A. veronii and A. caviae [10]. In particular, A. hydrophila and A. veronii cause hemorrhagic diseases in farmed fish, widely infect aquatic animals, and quickly become the epicenter of epidemic outbreaks, resulting in significant economic losses in aquaculture [11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Multilocus sequence typing and antibiotic resistance of Aeromonas isolated from freshwater fish in Hebei Province

Yu,

Oh,

Kim

et al. 2024

PLoS ONE

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“…The sample was then placed at 4°C for 5 h and centrifuged at 4° for 10 min at 2500 rpm, and the supernatant was transferred to new Eppendorf tubes. Total protein (TP), albumin (ALB), globulin (GLB), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (AKP) activities in the serum samples were accomplished using a fully automated biochemical analyser (Sysmex) (Hu et al, 2023;.…”

Section: Serum Biochemical Indicesmentioning

confidence: 99%

“…TP, ALB, and GLB levels decreased in the serum of P. sinensis infected with Aeromonas veronii, whereas AST and ALT levels increased (Hu et al, 2023). TP, ALB, AKP, and GLB levels were decreased in this study (p < 0.05).…”

Section: Reports Have Indicated That C Indologenes Caused Septicaemia Inmentioning

confidence: 99%

Isolation and identification of Chryseobacterium indologenes and its pathological changes in Pelodiscus sinensis

Xing,

Xue,

Xiao

et al. 2023

Journal of Fish Diseases

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The Chinese revered a species of aquatic reptile known as Pelodiscus sinensis as both an edible and medicinal species. When artificially breeding, many deaths occurred at the farmed P. sinensis, mainly due to excessive breeding density, water contamination, and turtles biting each other secondary to bacterial infections. In this study, an isolate of gram‐negative bacteria WH0623 was isolated from the liver and kidney of diseased P. sinensis to trace the potential pathogen of this disease. Based on biochemical characteristics and 16S rRNA gene sequencing analyses, this isolated strain of WH0623 was identified as Chryseobacterium indologenes. The strain's median lethal dose (LD50) was 3.3 × 105 colony‐forming units (CFU)/g per fish weight tested using artificial infection. Histopathological analysis revealed pathological changes, including cell swelling, hyperaemia, and necrosis in many tissues. Antibiotic susceptibility tests revealed that the bacteria WH0623 was susceptible to doxycycline, sulphonamides, ceftazidime, norfloxacin, and ciprofloxacin. These antibiotics could treat the disease. In conclusion, the pathogen causing the death of farmed P. sinensis was isolated and identified, and a drug‐sensitive test was conducted. Our findings contribute to the future diagnosis and treatment of the disease.

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“…Chinese soft‐shell turtle farming is associated with many infectious diseases during superintensive aquaculture growth (Zhou et al., 2008), including viruses, bacteria (Yuan et al., 2020) and fungi (Takuma et al., 2011), causing severe losses in this industry. These diseases are mainly caused by bacteria, such as Aeromonas hydrophila (Lin et al., 2014; Wu, 2004), Aeromonas caviae (Wu, 2004; Yu & Ma, 1997), Aeromonas sobria (Wu, 2004), Edwardsiella tarda (Pan et al., 2010), Lactococcus garvieae (Hu et al., 2010), Citrobacter freundii (Tang et al., 2023), Morganella morganii (Wei et al., 2023), Bacillus thuringiensis (Chen et al., 2014) and Bacillus cereus (Yuan et al., 2020).…”

Section: Introductionmentioning

confidence: 99%

Isolation, identification and characterization of Aeromonas jandaei from diseased Chinese soft‐shell turtles

Chen,

Xue,

Chen

et al. 2024

Journal of Fish Diseases

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Aeromonas jandaei is a gram‐negative bacterium commonly found in aquatic environments and can induce illnesses in amphibians, reptiles and aquatic animals. In this study, a strain of bacteria was isolated from the diseased Chinese soft‐shell turtle (Pelodiscus sinensis), then named strain JDP‐FX. This isolate was identified as A. jandaei after analysis of morphological, physiological and biochemical characteristics, as well as 16S rRNA and gyrB gene sequences. Virulence genetic testing further detected temperature‐sensitive protease (eprCAI), type III secretion system (TTSS) (ascv), nuclease (nuc), cytotonic enterotoxin (alt) and serine proteinase (ser) in JDP‐FX. Compared with healthy Chinese soft‐shell turtle, the serum levels of total protein (TP), albumin (ALB) and globulin (GLB) were significantly decreased in the diseased Chinese soft‐shell turtle, while, the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) were significantly increased. Histopathological observations showed that multiple tissues, including intestinal mucosa, liver and kidney, were severely damaged in the diseased Chinese soft‐shell turtle. Moreover, the diseased Chinese soft‐shell turtle had significant cell degeneration, necrosis, sloughing and interstitial inflammatory cell infiltration. The pathogenicity of JDP‐FX was tested via artificial infection. The median lethal dosage (LD50) of the strain was 1.05 × 105 colony forming units (CFU/g) per weight of Chinese soft‐shell turtle. Drug susceptibility analysis revealed that JDP‐FX was susceptible to ceftazidime, minocycline, cefoperazone, ceftriaxone and piperacillin. In addition, JDP‐FX was resistant to doxycycline, florfenicol, sulfonamides, gentamicin, ampicillin and neomycin. Therefore, this study may provide guidance for further research into the diagnosis, prevention and treatment of JDP‐FX infection.

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Isolation, Identification, and Characterization of Aeromonas veronii from Chinese Soft-Shelled Turtle (Trionyx sinensis)

Cited by 11 publications

References 32 publications

Multilocus sequence typing and antibiotic resistance of Aeromonas isolated from freshwater fish in Hebei Province

Multilocus sequence typing and antibiotic resistance of Aeromonas isolated from freshwater fish in Hebei Province

Isolation and identification of Chryseobacterium indologenes and its pathological changes in Pelodiscus sinensis

Isolation, identification and characterization of Aeromonas jandaei from diseased Chinese soft‐shell turtles

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