Isolation, Identification, and Comparison of Four Isolates of Avian Paramyxovirus Serotype 2 in China

Zhang, Guozhong; Zhao, Jinbiao; Wang, Hongwei; Yang, Anshu; Bu, Chunya; Wang, Ming

doi:10.1637/7502-010906r1.1

Cited by 27 publications

(26 citation statements)

References 22 publications

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“…These results are in accordance with data in the literature showing particles with similar morphology in pigeons (Gough & Alexander, 1983), owls (Catroxo et al, 2010), ducks (Chang et al, 2001) and Gouldian Finch (Zhang et al, 2006).…”

Section: Discussionsupporting

confidence: 93%

Research of Viral Agent in Free-living Pigeon Feces (Columba livia) in the City of São Paulo, SP, Brazil, for Transmission Electron Microscopy

et al. 2011

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SUMMARY:The pigeon feces are vehicle of diseases both for humans and other animal species. In these birds, the most important viral diseases of the digestive tract are transmitted by the paramyxovirus, adenovirus and coronavirus. Avian paramyxoviruses have been isolated from a variety of species of free living and domestic birds worldwide, with several symptoms and clinical signs and economic losses. Paramyxoviruses belong to the Paramyxoviridae family and Avulovirus genus that includes nine serotypes (APMV 1 to 9). Avian adenoviruses belong to the Adenoviridae family and Aviadenovirus genus. In pigeons, cause classical adenovirosis and necrotizing hepatitis. The respiratory and enteric tracts are common targets of coronavirus. They belong to the Coronaviridae family and to 3a and 3c groups. In this study, we described the presence of viral agents in free-living pigeon feces (Columba livia) from the city of São Paulo, Brazil. The feces were processed by negative staining technique (rapid preparation) for transmission electron microscopy. In this technique paramyxoviruses particles, pleomorphic, roughly spherical or filamentous, measuring 100 to 500 nm of diameter containing an envelope covered by spikes, with characteristic helical herring-bone-like nucleocapsid, measuring 15 to 20 nm in diameter, were visualized in 45 (79%) out of 57 feces samples. In 2 (3.5%) samples, paramyxovirus and adenovirus particles were simultaneously visualized. Adenovirus particles were isometric, spherical, characterized as "complete "or" empty ", measuring between 70 and 90 nm in diameter. Paramyxovirus and coronavirus particles were detected in 3 (5.2%) samples. Coronaviruses were pleomorphic with a diameter of 75-160 nm containing a solar corona-shaped envelope, with projections of approximately 20 nm of diameter. Seven (12.3%) samples were negative for viral particles.

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Section: Discussionsupporting

confidence: 93%

Research of Viral Agent in Free-living Pigeon Feces (Columba livia) in the City of São Paulo, SP, Brazil, for Transmission Electron Microscopy

et al. 2011

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“…In contrast, little is known about the potential pathogenic of APMV-2 to APMV-10 serotypes in captive or wild birds (Bankowski et al, 1968;Alexander et al, 1982;Zhang et al, 2006;Miller et al).…”

Section: Introductionmentioning

confidence: 99%

“…It was reported that in passerines and psittacines, the serotype 2 (APMV-2) frequently occurs triggering mild respiratory disease or high limiting, severe pneumonia, and diarrhea in passerines (Ritchie & Carter, 1995;Zhang et al) and weakness, weight loss, pneumonia, tracheitis, diarrhea with high mortality in psittacines (Collins et al, 1975, Ritchie).…”

Section: Introductionmentioning

confidence: 99%

Avian Paramyxoviruses: Detection by Transmission Electron Microscopy Techniques

Catroxo¹,

Martins²,

Petrella³

et al. 2012

Int. J. Morphol.

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SUMMARY:Diseases caused by avian paramyxovirus (APMV) occur in commercial, captive and wild birds worldwide, demonstrating the significant economic and ecological importance of these agents. Paramyxoviruses belong to the Paramyxoviridae family, Paramyxovirinae subfamily and Avulavirus genus. During the period 2000 to 2011, stool and small intestine samples of 1647 birds species were sent to the Laboratory of Electron Microscopy, Biological Institute of São Paulo, Brazil, for diagnosis of viral agents. The samples were processed by negative staining (rapid preparation) and resin embedding techniques. Under the transmission electron microscope by negative staining technique, in 294 (17.8%) samples of 1647 were visualized paramyxovirus particles pleomorphic, roughly spherical or filamentous, measuring 100 to 500 nm of diameter containing an envelope covered with spikes and characteristic helical herring-bone-like nucleocapsid measuring 15 to 20 nm in diameter. Ultrathin sections of the small intestine fragments revealed the presence of amorphous granular intracytoplasmic inclusions surrounded by membrane and containing viral nucleocapsid measuring 10-14 nm in diameter. Immature particles budding from cell membranes, pleomorphic, spherical and tubular particles containing viral nucleocapsid strands, and the complete particles measured up to 170 nm in diameter were seen in the cytoplasm. Intranuclear inclusions containing viral nucleocapsid were also visualized. Nuclei showed a marginalized chromatin.

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“…Since then many APMV-2 strains have been isolated from chickens, turkeys and feral birds across the globe (Alexander et al, 1982;Asahara et al, 1973;Collings et al, 1975;Fleury et al, 1979;Goodman et al, 1988;Lang et al, 1975;Lipkind et al, 1979;Lipkind et al, 1982;Mbugua et al, 1985;Nymadawa et al, 1977;Shihmanter et al, 1997;Weisman et al, 1984;Zhang et al, 2006;Zhang et al, 2007). Serological survey of chickens and turkeys has shown a wide prevalence of APMV-2 in the United States.…”

Section: Introductionmentioning

confidence: 99%

Complete sequence of the genome of avian paramyxovirus type 2 (strain Yucaipa) and comparison with other paramyxoviruses

et al. 2008

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The complete RNA genome sequence of avian paramyxovirus (APMV) serotype 2, strain Yucaipa isolated from chicken has been determined. With genome size of 14,904 nucleotides (nt), strain Yucaipa is consistent with the "rule of six" and is the smallest virus reported to date among the members of subfamily Paramyxovirinae. The genome contains six non-overlapping genes in the order 3′-N-P/V-M-F-HN-L-5′. The genes are flanked on either side by highly-conserved transcription start and stop signals and have intergenic sequences varying in length from 3 to 23 nt. The genome contains a 55 nt leader sequence at 3′ end and a 154 nt trailer sequence at 5′ end. Alignment and phylogenetic analysis of the predicted amino acid sequences of strain Yucaipa proteins with the cognate proteins of viruses of all of the five genera of family Paramyxoviridae showed that APMV-2 strain Yucaipa is more closely related to APMV-6 than APMV-1.

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Isolation, Identification, and Comparison of Four Isolates of Avian Paramyxovirus Serotype 2 in China

Cited by 27 publications

References 22 publications

Research of Viral Agent in Free-living Pigeon Feces (Columba livia) in the City of São Paulo, SP, Brazil, for Transmission Electron Microscopy

Research of Viral Agent in Free-living Pigeon Feces (Columba livia) in the City of São Paulo, SP, Brazil, for Transmission Electron Microscopy

Avian Paramyxoviruses: Detection by Transmission Electron Microscopy Techniques

Complete sequence of the genome of avian paramyxovirus type 2 (strain Yucaipa) and comparison with other paramyxoviruses

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