Isolation, identification and phylogenetic analysis of lumpy skin disease virus strain of outbreak in Guangdong, China

Wang, Jingyu; Xu, Zhiying; Wang, Zhen; Li, Qi; Liang, Xingling; Ye, Shaotang; Cheng, Kui; Xu, Liang; Mao, Jianwei; Wang, Zhiyuan; Meng, Wenxin; Sun, Yankuo; Jia, Kun; Li, Shoujun

doi:10.1111/tbed.14570

Cited by 17 publications

(19 citation statements)

References 61 publications

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“…In contrast to infection with bovine herpesvirus-2, which results in pseudo-lumpy skin condition and induces syncytia and intranuclear inclusion bodies in cell culture, LSDV causes a distinctive cytopathic effect and intracytoplasmic inclusion bodies [9]. According to Wang et al [47], the most sensitive cell line for the isolation of LSDV, is primary cattle testicular (PCT) cells, while vero cells cannot be used for the isolation of this virus.…”

Section: Virus Isolationmentioning

confidence: 99%

Lumpy Skin Disease: An Economically Significant Emerging Disease

Khalafalla¹

2023

Veterinary Medicine and Science

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Lumpy skin disease (LSD) is a severe viral disease of cattle caused by the lumpy skin disease virus (LSDV), a member of the Capripoxvirus genus of the poxviridae family. Fever and flat disk-like skin nodules on the skin characterize the disease. It can also lead to death and significant economic losses, especially in herds, that have never been exposed to the virus. Blood-feeding insects, such as specific types of flies, mosquitoes, and ticks, are thought to be the primary vectors of LSDV transmission. Most African and middle eastern countries have a high prevalence of lumpy skin disease. The disease extended to southeast Europe, the Balkans, and the Caucasus in 2015 and 2016 and is still spreading throughout Asia. The World Organization for Animal Health [WOAH] has designated LSD as a notifiable illness due to the likelihood of fast transmission. The rapid spread of disease in formerly disease-free areas emphasizes the need to know the disease epidemiology and the virus’s interaction with its host. This chapter aims to provide the latest developments in the etiology, epidemiology, diagnosis, and control of LSD.

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Section: Virus Isolationmentioning

confidence: 99%

Lumpy Skin Disease: An Economically Significant Emerging Disease

Khalafalla¹

2023

Veterinary Medicine and Science

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“…It also can multiply in Madin-Darby bovine kidney cells and baby hamster kidney cells (BHK-21), but the pathological changes are slower. Recent investigations have found that LSDV can hardly proliferate in African green monkey kidney (Vero) cells ( Wang et al, 2022 ). Kumar et al (2021) treated Vero cells with the product of virus amplification from primary goat kidney (PGK) cells, and it could obtain higher viral titer only after adapting to LSDV and continuing to pass generations.…”

Section: Etiologymentioning

confidence: 99%

“…LSDV can proliferate on the chorioallantoic membrane of chicken embryo and cause acne-spots, and the virus does not cause the death of the embryo ( Black et al, 1986 ; Upton, 2004 ; Kumar et al, 2021 ). The next year, Chinese scientists found that LSDV could produce higher viral titers in primary cattle testicular cells ( Wang et al, 2022 ). When the wild-type virus was attenuated to prepare the vaccine strains, the state of the chicken embryo can be observed as a reference.…”

Section: Etiologymentioning

confidence: 99%

Understanding the research advances on lumpy skin disease: A comprehensive literature review of experimental evidence

et al. 2022

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Lumpy skin disease is caused by lumpy skin disease virus (LSDV), which can induce cattle with high fever and extensive nodules on the mucosa or the scarfskin, seriously influencing the cattle industry development and international import and export trade. Since 2013, the disease has spread rapidly and widely throughout the Russia and Asia. In the past few decades, progress has been made in the study of LSDV. It is mainly transmitted by blood-sucking insects, and various modes of transmission with distinct seasonality. Figuring out how the virus spreads will help eradicate LSDV at its source. In the event of an outbreak, selecting the most effective vaccine to block and eliminate the threat posed by LSDV in a timely manner is the main choice for farmers and authorities. At present, a variety of vaccines for LSDV have been developed. The available vaccine products vary in quality, protection rate, safety and side effects. Early detection of LSDV can help reduce the cost of disease. In addition, because LSDV has a huge genome, it is currently also used as a vaccine carrier, forming a new complex with other viral genes through homologous recombination. The vaccine prepared based on this can have a certain preventive effect on many kinds of diseases. Clinical detection of disease including nucleic acid and antigen level. Each method varies in convenience, accuracy, cost, time and complexity of equipment. This article reviews our current understanding of the mode of transmission of LSDV and advances in vaccine types and detection methods, providing a background for further research into various aspects of LSDV in the future.

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“…This indicates the presence of either multiple Capripoxviruses and/or novel recombinant viruses in this vaccine. Despite all the evidence for multiple new recombination events in LSDV, a single lineage has survived selection and established itself in southeast Asia ( Tran et al, 2021 ; Wang et al, 2022 ). Named after the first detection in China in 2019, the vast expansion of viruses belonging to this Chinese lineage, might be due to either enhanced viral fitness allowing domination over other genetic variants or because it spread into a susceptible cattle population in the absence of competing LSDVs.…”

Section: Poxvirus Recombinants In the Fieldmentioning

confidence: 99%

Capripoxviruses, leporipoxviruses, and orthopoxviruses: Occurrences of recombination

et al. 2022

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Poxviruses are double-stranded DNA viruses with several members displaying restricted host ranges. They are genetically stable with low nucleotide mutation rates compared to other viruses, due to the poxviral high-fidelity DNA polymerase. Despite the low accumulation of mutations per replication cycle, poxvirus genomes can recombine with each other to generate genetically rearranged viruses through recombination, a process directly associated with replication and the aforementioned DNA polymerase. Orthopoxvirus replication is intimately tethered to high frequencies of homologous recombination between co-infecting viruses, duplicated sequences of the same virus, and plasmid DNA transfected into poxvirus-infected cells. Unfortunately, the effect of these genomic alterations on the cellular context for all poxviruses across the family Poxviridae remains elusive. However, emerging sequence data on currently circulating and archived poxviruses, such as the genera orthopoxviruses and capripoxviruses, display a wide degree of divergence. This genetic variability cannot be explained by clonality or genetic drift alone, but are probably a result of significant genomic alterations, such as homologous recombination, gene loss and gain, or gene duplications as the major selection forces acting on viral progeny. The objective of this review is to cross-sectionally overview the currently available findings on natural and laboratory observations of recombination in orthopoxviruses, capripoxviruses, and leporipoxviruses, as well as the possible mechanisms involved. Overall, the reviewed available evidence allows us to conclude that the current state of knowledge is limited in terms of the relevance of genetic variations across even a genus of poxviruses as well as fundamental features governing and precipitating intrinsic gene flow and recombination events.

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Isolation, identification and phylogenetic analysis of lumpy skin disease virus strain of outbreak in Guangdong, China

Cited by 17 publications

References 61 publications

Lumpy Skin Disease: An Economically Significant Emerging Disease

Lumpy Skin Disease: An Economically Significant Emerging Disease

Understanding the research advances on lumpy skin disease: A comprehensive literature review of experimental evidence

Capripoxviruses, leporipoxviruses, and orthopoxviruses: Occurrences of recombination

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