The current study was conducted to isolate and diagnose the bacteria that company cases ocular myiasis as complications. The bacteria were collected using a cotton swab for 30 individuals who came to the Diwaniyah Teaching Hospital due to their infection with ocularmyiasis. The swabs were cultivated on many culture media, such as blood agar and MacConkey, and mannitol salt and EMB medium for diagnosis of Ecoli. The results of the culture were positive for all samples under study. Various types were isolated; including rod and coccobacilli gram-negative in addition to gram-positive cocci, fermented and non-fermented mannitol species, and the identification of the species was confirmed using the VITEK2 system, the antibiotic susceptibility test was also checked using the same test and the results were as the following Ecoli in 5 cases, Pseudomonas spp in 4 cases, Kelbssilla pneumonia in one case, S aureus in 4 cases, S.epidermides in 5 cases in addition to many other different species.