Isolation, molecular identification and antibiotic susceptibility profile of Aeromonas hydrophila from cultured indigenous Koi (Anabus testudineus) of Bangladesh

Borty, Shuvho Chakra; Rahman, Fabaya; Reza, Asadi Karam Mohammad; Khatun, Maksuda; Kabir, Md. Luthful; Rahman, Muhammed Hafizur; Monir, Md. Shirajum

doi:10.3329/ajmbr.v2i2.29078

Cited by 11 publications

(13 citation statements)

References 1 publication

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“…Yarmidici and Aydin (2011) noticed body rubbing against tank walls in Nile tilapia infected with A. hydrophila isolate after 8 hours of infection. However, similar observations were also documented by Borty et al (2016); Monir et al (2015).…”

Section: Discussionsupporting

confidence: 86%

Evaluation of pathogenicity of motile Aeromonas species in air-breathing catfish Magur (Clarias batrachus)

Monir¹,

Bagum²,

Kabir³

et al. 2017

Asian Australas. J. Food. Saf. Secur.

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The present study was carried out to evaluate the comparative capability of producing infections and causing mortality of the experimental Magur (Clarias batrachus) with motile Aeromonas species. A total of 200 apparently healthy C. batrachus were acclimatized to the laboratory conditions for experimental study. Nine different groups (each group consisting of 20 fish) of healthy C. batrachus was injected with nine motile Aeromonas isolates (A. hydrophila-3, A. sobria-3 and A. caviae-3). Experimental C. batrachus were infected with motile A. hydrophila, A. sobria and A. caviae to groups 1-3, 4-6 and 7-9, respectively while group 10 was injected with sterile physiological saline (0.85% NaCl) and served as the control. The selected motile bacterial species via intramuscularly were injected at the rate of 4.5 × 105 cfu/fish for pathogenicity study on C. batrachus and monitored up to two weeks. The highest clinical infections were noticed 90% in group-3 whereas only 35% in group-8 within the experimental period. After two weeks of the experiment, the cumulative mortality rate was also found highest (60%) in group-3 but lowest (15%) in group-9 while no infection or mortality showed in group-10 (control group). The development of infection and mortality to the injected C. batrachus was associated more severely by Aeromonas hydrophila than A. sobria and A. caviae used in this study. However, the isolates motile Aeromonas species could serve as the primary cause of skin lesions as well as mortality in cultured C. batrachus. Asian Australas. J. Food Saf. Secur. 2017, 1 (1), 45-50

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Section: Discussionsupporting

confidence: 86%

Evaluation of pathogenicity of motile Aeromonas species in air-breathing catfish Magur (Clarias batrachus)

Monir¹,

Bagum²,

Kabir³

et al. 2017

Asian Australas. J. Food. Saf. Secur.

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“…It showed the best result (90% recovery of fish). The relevant results were found by the study of Sharmin et al (2016); Hossain et al (2016); Monir et al (2015); Chowdhury et al (2015); Ahmed et al (2015); Hasan et al (2015) and Borty et al (2016). Scott and Warren (1964) used 2 ppm concentration of methylene blue for complete destruction of fungal hyphae within 7 days treatment.…”

Section: Discussionmentioning

confidence: 77%

Effects of chemotherapeutics against experimentally injured stinging catfish Heteropneustes fossilis

Neowajh

Rashid

Asif

et al. 2018

Asian J. Med. Biol. Res

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Effects of different chemotherapeutics were examined against experimentally infected stinging catfish Heteropneustes fossilis. Fish were collected from a fish market in Mymensingh, acclimatized for 7 days in laboratory condition from January to February, 2016 in aquaria at Fish Clinic of Bangladesh Agricultural University, Mymensingh, experimentally injured by using forceps and knife and waited seven days for the establishment of infection. Before starting chemotherapeutic trial, it was confirmed that the fish were infected with microorganisms by clinical diagnosis in laboratory condition. They showed hemorrhages and ulcerative lesions over the infected area. A total of 80 such experimentally infected stinging catfish having average body weight of 18 g were used for the experiment. Two chemotherapeutics: antibiotic, Eryvet (erythromycin thiocyanate INN, sulphadiazine (NaUSP) & trimithoprim BP) and antifungal, methylene blue were used in separate and combined treatment with three different doses of antibiotic. The same dose of methylene blue, 0.2 mg/l, was used for separate and combined treatment by antibiotic. Doses of antibiotic (Eryvet) were 0.8 g/10 kg body weight of fish, 1g/10 kg body weight of fish and 1.2 g/10 kg body weight of fish as lower dose, recommended dose and higher dose respectively. For combined treatment the above different doses of antibiotic and the same dose of antifungal were used. Water was exchanged regularly. The chemotherapeutic trial was conducted for 7 days and observation was continued for another 8 days to observe the effect of treatment. Combined treatment with the recommended dose of the antibiotic and methylene blue showed the best result where 90% fish were recovered. By the treatment with the higher dose of the antibiotic 70% fish were recovered. Antifungal treatment showed that 20% fish were recovered. All the fish in negative control aquarium died.

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“…Recorded that histopathological examination of infected eggs showed numerous fungal hyphae on the outer surface of the eggs and may penetrate the egg envelope and in some cases infected eggs showed germinated zoospores in the cytoplasm. This may return to the characterization of damaged dead egg or degradation of the egg envelope structural components by fungi released enzyme that facilitate hyphae penetrated across the egg envelope and accumulated in the cytoplasm [32,33].…”

Section: Discussionmentioning

confidence: 99%

Identification of Causative Agent for Fungal Infection and Effect of Disinfectants on Hatching and Survival Rate of Bata (Labeo. Bata) Larvae

Rahman¹,

Rahman²,

Yeasmin³

et al. 2017

APAR

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Labeo. bata is one of the most important cultured fish. Intensive incubation leads to microbial overgrowth in L. bata eggs that hamper egg development hatchability and larval survivability. The aim of this study is to find out causes of mass mortality in L. bata eggs during peak breeding season from 10 March to May 2015 at Mafatema Fish Hatchery Jessore Bangladesh. Three concentrations of four chemical-formalin (10 20 30 mg/L) malachite green (135 mg/L) NaCl (123 g/l) and methylene blue (135 mg/L) treatment regimes and a control were compared for efficacy in treating L. bata eggs to prevent fungus and bacterial infection and improve hatch and survival rate of fry. Physicochemical and microbial characteristics of culture water were examined during the induced breeding of L. bata besides mycological examination of egg samples with trial of treatment of different types of disinfectant. The total bacterial count fluctuated between 3.6×10 8 cfu/ml at initial time of incubation and 31.7×108 cfu/ml after 3 days of hatching. The infected fertilized egg by Saprolegnia sp. were appeared as tuft hairy like balls with a white cottony envelope that surround it which focally invaded the cytoplasm resulted in loss of the cytoplasm content and destructed envelops.

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Isolation, molecular identification and antibiotic susceptibility profile of Aeromonas hydrophila from cultured indigenous Koi (Anabus testudineus) of Bangladesh

Cited by 11 publications

References 1 publication

Evaluation of pathogenicity of motile Aeromonas species in air-breathing catfish Magur (Clarias batrachus)

Evaluation of pathogenicity of motile Aeromonas species in air-breathing catfish Magur (Clarias batrachus)

Effects of chemotherapeutics against experimentally injured stinging catfish Heteropneustes fossilis

Identification of Causative Agent for Fungal Infection and Effect of Disinfectants on Hatching and Survival Rate of Bata (Labeo. Bata) Larvae

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