Isolation, molecular identification and antibiotics susceptibility of Salmonella serotypes isolates from bats in Osun State, Nigeria

Aladejana, Oluwatoyin Modupe; Oluduro, Anthonia Olufunke; Ogunlade, Ayodele Oluwayemisi; Babatunde, Shola Kola; Sanni, Olufunke Tolulope

doi:10.1007/s42535-023-00791-2

Vegetos

2024

DOI: 10.1007/s42535-023-00791-2

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Isolation, molecular identification and antibiotics susceptibility of Salmonella serotypes isolates from bats in Osun State, Nigeria

Oluwatoyin Modupe Aladejana,

Anthonia Olufunke Oluduro,

Ayodele Oluwayemisi Ogunlade

et al.

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Cited by 1 publication

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Plasmid Profile and Curing of Multiple Antibiotic Resistant Escherichia coli Isolated from Straw Colored Fruit Bats (Eidolon helvum )

Aladejana,

Ogunlade,

Thonda

et al. 2024

NJPAS

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Plasmids are well recognized to play vital roles in developing and spreading multi-drug resistance in bacteria, which pose a risk to veterinary and human health. Six multi-drug-resistant Escherichia coli were gotten from Eidolon helvum from Osun State, Nigeria. Analytical Profile Index (API) 20E kit for biochemical identification was used to confirm the identity of the isolates. Antibiotic susceptibility test using eleven different antibiotics was performed using Kirby-Bauer’s disc diffusion technique. The Escherichia coli was analyzed for their plasmids, and plasmid curing was done using ethidium bromide and sodium dodecyl sulphate. The results of the API 20E kits confirmed the isolates to be Escherichia coli. They were 100% resistant to ampicillin, augmentin, ceftazidime, cefuroxime, ertapenem, sulfamethoxazole, and cefixime and were also 100% susceptible to gentamicin, ciprofloxacin, ofloxacin, and nitrofurantoin before and after curing. Plasmid analysis revealed that all the isolates except one had plasmid. The plasmid molecular weight ranged from 9.961(kpb) to 9.369 (kpb) for the untreated isolates. while isolates treated with sodium dodecyl sulphate and ethidium bromide still had bands but with reduced molecular weight, ranging from 8.369 (kpb) to 8.462 (kpb) and 8.369 (kpb) to 8.574 (kpb) respectively. The exhibition of the same susceptibility pattern before and after curing the plasmid present in the Escherichia coli from Eidolon helvum studied suggests that they are chromosomally mediated, indicating that resistance can occur naturally in nature. Also, the results of the curing agents indicated that sodium dodecyl sulphate is a better curing agent than ethidium bromide.

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Plasmid Profile and Curing of Multiple Antibiotic Resistant Escherichia coli Isolated from Straw Colored Fruit Bats (Eidolon helvum )

Aladejana,

Ogunlade,

Thonda

et al. 2024

NJPAS

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show abstract

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Isolation, molecular identification and antibiotics susceptibility of Salmonella serotypes isolates from bats in Osun State, Nigeria

Cited by 1 publication

References 20 publications

Plasmid Profile and Curing of Multiple Antibiotic Resistant Escherichia coli Isolated from Straw Colored Fruit Bats (Eidolon helvum )

Plasmid Profile and Curing of Multiple Antibiotic Resistant Escherichia coli Isolated from Straw Colored Fruit Bats (Eidolon helvum )

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