Isolation of a basic 34 kiloDalton β-1,3-glucanase with inhibitory activity againstPhytophthora capsicifrom pepper stems

Kim, Young Jin; Hwang, Byung Kook

doi:10.1006/pmpp.1996.0073

Cited by 63 publications

(33 citation statements)

References 26 publications

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“…The accumulation of H 2 O 2 has already been demonstrated as a defense response that inhibits S. tritici colonization during its initial biotrophic phase (13,20). Moreover, it is noteworthy that the accumulation of PR-1 and PR-2 was observed in both cultivars, whereas PR-3 was accumulated only in Sevin, despite the fact that PR-2 and PR-3 function in the degradation of fungal cell walls (40). This indicates the stronger involvement of PR-2 than PR-3 in defense responses to S. tritici and that released ␤-1,3-glucan probably acts as the elicitor of further defense reactions during both compatible and incompatible interactions, as shown previously (19).…”

Section: Discussionmentioning

confidence: 87%

Battle through Signaling between Wheat and the Fungal Pathogen Septoria tritici Revealed by Proteomics and Phosphoproteomics

Yang

Melo‐Braga

Larsen

et al. 2013

Molecular & Cellular Proteomics

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The fungus Septoria tritici causes the disease septoria tritici blotch in wheat, one of the most economically devastating foliar diseases in this crop. To investigate signaling events and defense responses in the wheat-S. tritici interaction, we performed a time-course study of S. tritici infection in resistant and susceptible wheat using quantitative proteomics and phosphoproteomics, with special emphasis on the initial biotrophic phase of interactions. Our study revealed an accumulation of defense and stress-related proteins, suppression of photosynthesis, and changes in sugar metabolism during compatible and incompatible interactions. However, differential regulation of the phosphorylation status of signaling proteins, transcription and translation regulators, and membraneassociated proteins was observed between two interactions. The proteomic data were correlated with a more rapid or stronger accumulation of signal molecules, including calcium, H 2 O 2 , NO, and sugars, in the resistant than in the susceptible cultivar in response to the infection. Additionally, 31 proteins and 5 phosphoproteins from the pathogen were identified, including metabolic proteins and signaling proteins such as GTP-binding proteins, 14 -3-3 proteins, and calcium-binding proteins. Quantitative PCR analysis showed the expression of fungal signaling genes and genes encoding a superoxide dismutase and cell-wall degrading enzymes. These results indicate roles of signaling, antioxidative stress mechanisms, and nutrient acquisition in facilitating the initial symptomless growth. Taken in its entirety, our dataset suggests interplay between the plant and S. tritici through complex signaling networks and downstream molecular events. Resistance is likely related to several rapidly and intensively triggered signal transduction cascades resulting in a multiple-level activation of transcription and translation processes of defense responses. Our sensitive approaches and model provide a comprehensive (phospho)proteomics resource for studying signaling from the point of view of both host and pathogen during a plant-pathogen interaction. Molecular & Cellular Proteomics 12:

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Section: Discussionmentioning

confidence: 87%

Battle through Signaling between Wheat and the Fungal Pathogen Septoria tritici Revealed by Proteomics and Phosphoproteomics

Yang

Melo‐Braga

Larsen

et al. 2013

Molecular & Cellular Proteomics

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“…These results suggest that SAP1 is associated with a transport system in the host bacterial cells (54), while SAP3 participates in bacterial iron storage with its possible heme structure (3,10,21,43). There have been no reports on the proteins active against Pythium spp., but two proteins active against Phytophthora, a chitin-negative fungal genus related to Pythium, have been reported (26,39). These 14-and 34-kDa proteins were identified as basic pathogenesis-related protein 1 from tomato (Lycopersicon esculentum Mill.…”

Section: Resultsmentioning

confidence: 99%

An Antifungal Protein from the Marine Bacterium Streptomyces sp. Strain AP77 Is Specific for Pythium porphyrae , a Causative Agent of Red Rot Disease in Porphyra spp

Woo

Kitamura

Myouga

et al. 2002

Appl Environ Microbiol

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A novel antifungal protein (SAP) was found in the culture supernatant of a marine bacterium, Streptomyces sp. strain AP77, and was purified. This protein was characterized by chemical, biochemical, and biological analyses. By using gel filtration, the molecular mass of SAP was estimated to be 160 kDa. Structural analysis of SAP by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and matrix-assisted laser desorption ionization-time of flight mass spectrometry suggested that SAP is composed of three heterologous protein subunits of 41.7 kDa (SAP1), 21.7 kDa (SAP2), and 18.7 kDa (SAP3) at a molar ratio of 1:1:5 (or 1:1:6). N-terminal amino acid sequence analysis and a homology search revealed that SAP1, SAP2, and SAP3 exhibit 64.3, 68.4, and 86.7% similarity to three Streptomyces coelicolor polypeptides, puromycin resistance protein (Pur8), a conserved hypothetical protein, and bacterioferritin, respectively. The MIC of purified SAP against Pythium porphyrae was determined to be 1.6 g/disk, whereas no inhibitory effect was observed at concentrations up to 100 g/disk against most of the fungal and bacterial strains tested; the only exception was relatively strong antifungal activity against Pythium ultimum (MIC, 6.3 g/disk). In vitro and in vivo toxicity tests demonstrated that SAP showed no toxicity against Porphyra yezoensis cells, human normal dermal fibroblasts, and mice at doses up to 700 g/ml (for 24 h), 250 g/ml (for 12 h), and 75 mg/kg (for 35 days), respectively. SAP was labile when it was subjected to a heated-air drying treatment, which is a great advantage in food production procedures. These results indicated that Streptomyces sp. strain AP77 might be useful as a gene source for safe transgenic Porphyra breeding for tolerance to Pythium infection.

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“…The major structural difference between class I proteins and the other two classes is that class I proteins are synthesized as preproproteins that are processed prior to being enzymatically active. PR-2 proteins have been found in a wide variety of plants, including tobacco, A. thaliana, peas, grains, and fruits (25,77,146); the proteins are active in vitro at micromolar levels (ϳ50 g/ml) against a wide number of fungi, including human and plant pathogens (e.g., Rhizoctonia solani, C. albicans, and Aspergillus fumigatus). The antifungal activity of PR-2 proteins has been convincingly demonstrated by a number of in vitro enzyme and whole-cell assays (163) as well as in planta using transgenic plants overexpressing a PR-2 protein (71).…”

Section: Fungal Cell Wall Structurementioning

confidence: 99%

Antifungal Proteins

Selitrennikoff

2001

Appl Environ Microbiol

529

362

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Isolation of a basic 34 kiloDalton β-1,3-glucanase with inhibitory activity againstPhytophthora capsicifrom pepper stems

Cited by 63 publications

References 26 publications

Battle through Signaling between Wheat and the Fungal Pathogen Septoria tritici Revealed by Proteomics and Phosphoproteomics

Battle through Signaling between Wheat and the Fungal Pathogen Septoria tritici Revealed by Proteomics and Phosphoproteomics

An Antifungal Protein from the Marine Bacterium Streptomyces sp. Strain AP77 Is Specific for Pythium porphyrae , a Causative Agent of Red Rot Disease in Porphyra spp

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