Isolation of a biphenyl-degrading bacterium, Achromobacter sp. BP3, and cloning of the bph gene cluster

Potential for biodegradation of polychlorinated biphenyls (PCBs) by Sinorhizobium meliloti

“…Quantitative detection for yellow intermediate HOPDA (2-hydroxy-6-oxo-6-phenylhex-2,4-dienoic acid) was determined by measuring A 430 of the culture on a UV-visible scanning spectrophotometer (T6, PGENERAL, China) [21].…”

Draft Genome Sequence of Cupriavidus sp. Strain SK-4, a di- ortho -Substituted Biphenyl-Utilizing Bacterium Isolated from Polychlorinated Biphenyl-Contaminated Sludge

“…The utilization of microorganisms that are capable of metabolizing PCB compounds might become an efficient and less aggressive alternative for disposing of PCB pollutants (3). However, bioremediation methods often fail because of the accumulation of ortho - and para -substituted PCB compounds (1).…”

Cloning of three 2,3-dihydroxybiphenyl-1,2-dioxygenase genes from Achromobacter sp. BP3 and the analysis of their roles in the biodegradation of biphenyl

“…BP3 used in this study was from our laboratory, which was isolated from petroleum-contaminated soil [18]. E. coli DH5␣ and E. coli BL21 (DE3) were used as hosts for constructing genomic library and heterologous expression of protein, respectively.…”

“…Finally, a continuous 16,709 bp DNA fragment (GenBank Accession No. EU81271) consisting of the entire bph gene cluster (bphRA1A2XA3A4BC1KHJID) was obtained based on the splicing of all of these fragments [18]. The bphC1 gene was 897 bp in length, and its amino acid sequence showed 100% similarity with BphC of Burkholderia xenovorans LB400 (GenBank Accession No.…”

“…In our previous study, a biphenyl-degrading strain Achromobacter sp. BP3 was isolated from petroleum-contaminated soil, which was an efficient degrader of biphenyl [18]. In the present study, three 2,3-dihydroxybiphenyl 1,2-dioxygenase genes (designated as bphC1, bphC2 and bphC3) were cloned from Achromobacter sp.…”