Isolation of a bovine rotavirus with a "super-short" RNA electrophoretic pattern from a calf with diarrhea

Abstract

A rotavirus with a "super-short" RNA electropherotype was isolated from a calf with diarrhea and was designated VMRI strain. Segments 10 and Il of this rotavirus migrated more slowly than did those of bovine rotavirus strains NCDV, B641, and B223. The electrophoretic pattern of the VMRI strain was similar to that reported for rotaviruses with super-short RNA electropherotypes from humans and rabbits. Northern (RNA) blot hybridization indicated that gene 11 of the VMRI strain was altered and migrated between gene segments 9 and 10. The subgroup of the VMRI strain was shown to be subgroup I. The VMRI strain of bovine rotavirus was neutralized by antisera containing polyclonal antibodies to rotavirus serotype 6 (bovine rotavirus serotype I) strains NCDV and B641 and by ascitic fluid containing monoclonal antibodies directed to VP7 of serotype 6 rotavirus. The VMRI strain was not neutralized by either polyclonal or monoclonal antibodies to strain B223 (bovine rotavirus serotype II). Collective data on the neutralization of the VMRI strain with monoclonal antibodies and polyclonal antibodies suggest that this virus is a member of the NCDV group (serotype 6) of rotaviruses (bovine rotavirus serotype 1).

Prevalence of group A and group B rotaviruses in the feces of neonatal dairy calves from California

“…The results from PAGE analysis showing that none of the 25 PAGE positive samples had the vaccine virus electropherotype agreed with a previous report which suggested that the vaccine was probably not the cause of rotaviral diarrhea, and it was frequently inefficacious in preventing the infections in the herds [14]. Short electropherotype rotaviruses similar to the rotaviruses previously reported in humans, rabbits, pigs and calves [12][13][14][15][16][17][18][19] were found in all day 1 PAGE positive and most of day 7 PAGE positive samples obtained from calves while long electropherotype rotaviruses were found at days 7 and 14. Regardless of their electropherotypes, the presence of these short and long electropherotype rotaviruses might be due to their antigenic difference from common rotaviruses contaminating the farm environment; thus, they were able to evade passive immunity.…”

“…The high incidence of short electropherotype group A rotavirus in calves was unexpected. A few cases of short electropherotype and a mixture of short and long electropherotype rotaviruses were previously reported in diarrheic calves [12,13,15,16].…”

“…"Short" and "supershort" electropherotypes refer to group A rotaviruses whose genome segment 11, and genome segments 10 and 11 respectively migrate at a relatively slow rate [11]. Super-short electropherotype rotavirus isolated from a calf, however, was demonstrated to be the result of the altered mobility of genome segment 1 I which migrated between genome segments 9 and 10 [12]. Short electropherotypes have been recognized in mice, rabbits, calves, humans, pigs and birds [5,[12][13][14][15][16][17][18][19][20][21].…”

Some infectious causes of diarrhea in young farm animals.

“…All bovine group A rotaviruses characterized thus far have the subgroup 1 antigen, and most exhibit a long RNA electropherotype pattern (143,144). Two recent studies have shown the occurrence of bovine group A rotaviruses with the subgroup 1 antigen and short electropherotype patterns (283,363). Short RNA electropherotype patterns had not been detected previously in bovine isolates but have been observed frequently in human rotavirus strains.…”

Development of specific nucleic acid probes for the differentiation of porcine rotavirus serotypes

“…Viral RNA was extracted from fecal samples as previously described (Paul et al, 1988b ). Briefly, 0.2 g fecal samples were diluted with 0.6 ml of sodium acetate buffer, sonicated and clarified by centrifugation at 13 000Xg for 1 min.…”