Isolation of a canine herpes virus

Prydie, J; Mj, Harrison; Graham, Jessica B.

doi:10.1136/vr.79.22.660

Cited by 18 publications

(15 citation statements)

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“…Other reports on canine herpesvirus (CHV-1) soon followed [3], [4] and [5]. CHV-1 is of low pathogenicity in puppies older than 5 weeks [6] and may cause tracheobronchitis in adult dogs [7].…”

Section: Introductionmentioning

confidence: 99%

Seroprevalence of canine herpesvirus in breeding kennels in the Gauteng Province of South Africa

et al. 2008

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Canine herpesvirus (CHV-1) causes neonatal deaths as well as infertility due to embryonal death, abortion and stillbirths in breeding kennels. The aim of this study was to determine the prevalence of antibodies against canine herpesvirus in the serum of dogs older than 1 year in breeding kennels in the Gauteng Province of South Africa. A serum neutralization test (SNT) and a newly developed enzyme linked immunosorbent assay (ELISA) were used to test the serum samples of 328 dogs in 38 breeding kennels. With SNT as well as ELISA, 22% of sera were positive (P > 0.9). Seventeen kennels (45% of total kennels) each had at least one positive dog on SNT compared with twenty kennels (53% of total kennels) that each had at least one positive dog on ELISA (P = 0.6). The prevalence of positive dogs in positive kennels was 42 ± 26% (n = 17 kennels) for SNT and 39 ± 26% (n = 20 kennels) for ELISA. Pairwise comparison of kennels showed that the prevalence of SNT positive dogs was similar to the prevalence of ELISA positive dogs (P = 0.3, n = 38 kennels). Seroprevalence was independent of age, gender or colony size. This study suggests that canine herpesvirus is sufficiently common in breeding dogs in the Gauteng Province of South Africa to pose a threat to neonatal survival and fertility.

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Section: Introductionmentioning

confidence: 99%

Seroprevalence of canine herpesvirus in breeding kennels in the Gauteng Province of South Africa

et al. 2008

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“…Early reports showed no neutralization [19] or very low levels of neutralization [5] of CHV by immune sera prepared against FHV-1 and no neutralization of FHV-1 by immune sera prepared against CHV [5].…”

Section: Discussionmentioning

confidence: 99%

“…More importantly, because of their rote in virus infection and immunity, serological and antigenic cross-reactions between glycoproteins among herpesviruses have also been described [2, 4, 6, t3, 16, 22, 28]. Earlier workers found no cross-neutralization between CHV and FHV-1 [19]. However, a hint of a uni-directional neutralization of CHV by FHV-1 antibodies has been reported [5].…”

Section: Introductionmentioning

confidence: 96%

Immunological relationship between feline herpesvirus type 1 (FHV-1) and canine herpesvirus (CHV) as revealed by polyvalent and monoclonal antibodies

Limcumpao

Horimoto

Xuan

et al. 1990

Archives of Virology

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Polyvalent and monoclonal antibodies were used to demonstrate serological and antigenic relationships between feline herpesvirus type 1 (FHV-1) and canine herpesvirus (CHV). Using virus neutralization tests, enzyme-linked immunosorbent and indirect immunofluorescence assays, and immunoblotting analysis, reciprocal cross-reactivities to the heterologous virus were observed with some polyvalent and monoclonal antibodies. One monoclonal antibody against CHV neutralized FHV-1 infectivity and one monoclonal antibody against FHV-1 inhibited the hemagglutination activity of CHV as well as FHV-1-infected mouse serum. The major cross-reacting proteins were identified as the 143/108 kDa and 60 kDa glycoproteins of FHV-1 and the 145/112 kDa and 41 kDa glycoproteins of CHV. Previously, we have identified the 60 kDa and 41 kDa glycoproteins as the hemagglutinins of FHV-1 and CHV, respectively. The present results indicated the presence of shared antigenic determinants among these proteins.

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“…In neonatal puppies, the virus can produce a systemic fatal haemorrhagic infection causing focal necrosis in parenchymatous organs of puppies up to 2-weeks-old [3,4]. CHV1 is of low pathogenicity in puppies older than 5 weeks [5].…”

Section: Introductionmentioning

confidence: 99%

“…CHV1 is of low pathogenicity in puppies older than 5 weeks [5]. In the adult dog, the infection is usually asymptomatic, but may cause upper respiratory infection [3] and ocular disease [6,7]. However, in the bitch herpesvirus infection has been associated with reproductive problems, such as low conception rate, fetal resorption, abortion, stillborn or weak puppies and small litter size both in experimental [8,9] and epidemiological studies [10-12].…”

Section: Introductionmentioning

confidence: 99%

A serological study of canine herpesvirus-1 infection in a population of breeding bitches in Norway

et al. 2014

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BackgroundCanine herpesvirus-1 (CHV1) causes a fatal hemorrhagic disease in neonatal puppies and is associated with infertility in female dogs. This study was conducted to assess the status of CHV1 infection in bitches in proestrus or estrus and to investigate possible risk factors by a detailed questionnaire. Blood samples were collected from healthy bitches (n = 193) not vaccinated against CHV1, aged one year or older and admitted for estrus control to the Canine Reproductive Clinical Unit, Norwegian School of Veterinary Science. The serum samples were analysed by immunoperoxidase monolayer assay and serum titers were recorded as the reciprocal value of the highest dilution producing specific cell staining.ResultsAltogether, 85.5% of the dogs had CHV1 titers ≥ 80 and were classified as positive. Mean age for dogs included in the study was 4.2 years (95% CI 4.0-4.5), and there was no difference in age between seronegative dogs vs seropositive dogs. When grouping the seropositive dogs into three categories according to the magnitude of the titer, a total of 38.8% of the bitches displayed a weakly positive titer of 80, 44.8% had moderately positive titers of 160 or 320 and 16.4% of the dogs fell into the strongly positive category with titer of ≥640. No association was demonstrated when comparing CHV1 antibody titers to fertility parameters such as previous matings, pregnancies, whelpings, puppies born or condition of puppies. Further, there was no difference in seroprevalence between bitches that had been abroad for a period of time and dogs only living within a Norwegian environment. Samples from dogs collected in summer and fall displayed moderate to high antibody titers indicating recent infection with CHV1. Season, previous birth, and participation in competitions/shows explained 67-78% of the variation in antibody titer.ConclusionsThis study demonstrates that CHV1 infection is common in breeding bitches in the eastern part of Norway. Associations with putative risk factors were not identified. However, season, previous whelping, and participation in competitions/shows explained 67-78% of the variation in antibody titer.

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Isolation of a canine herpes virus

Cited by 18 publications

References 0 publications

Seroprevalence of canine herpesvirus in breeding kennels in the Gauteng Province of South Africa

Seroprevalence of canine herpesvirus in breeding kennels in the Gauteng Province of South Africa

Immunological relationship between feline herpesvirus type 1 (FHV-1) and canine herpesvirus (CHV) as revealed by polyvalent and monoclonal antibodies

A serological study of canine herpesvirus-1 infection in a population of breeding bitches in Norway

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