Isolation of a capsid protein of bluetongue virus that induces a protective immune response in sheep

Huismans, H.; Walt, N.T. Van der; Cloete, M.; Erasmus, B. J.

doi:10.1016/0042-6822(87)90326-6

Cited by 204 publications

(115 citation statements)

References 15 publications

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“…Together these results emphasize prior observations that infection with one serotype does not necessarily confer protection against infection with another (Howell, 1960;Howell et al, 1970;Jeggo and Wardley, 1985;Huismans et al, 1987;Eschbaumer et al, 2011) and highlights the requirement for the pre-screening of animals by using both molecular and serological methods prior to inclusion in BTV challenge studies.…”

Section: Discussionsupporting

confidence: 82%

“…Twenty six serotypes of the virus have been identified (Howell, 1960;Howell et al, 1970;Maan et al, 2007;Hofmann et al, 2008;Maan et al, 2011) based either on serotype-specific neutralisation of the viral outer capsid protein VP2 (Huismans and Erasmus, 1981;Huismans et al, 1987;Mertens et al, 1989) or analysis of VP2 amino acid sequence data (Hoffman et al, 2008;Maan et al, 2007;Maan et al, 2011). Bluetongue virus is transmitted primarily through the bites of haematophagous midges (Culicoides spp.…”

Section: Introductionmentioning

confidence: 99%

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Transplacental infection in goats experimentally infected with a European strain of bluetongue virus serotype 8

Coetzee

Stokstad

Myrmel

et al. 2013

The Veterinary Journal

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The capability of the current European strain of bluetongue virus serotype 8 (BTV-8) to cross the ruminant placenta has been established in experimental and field studies in both sheep and cattle. Sero-prevalence rates in goats in north-western Europe were high during the recent outbreak of BTV-8, however the capability of the virus to infect goats through the transplacental route has not been established. In the present study, four Saanen goats pregnant at 62 days of gestation were inoculated with the European strain of BTV-8, in order to investigate the capability of the virus to cross the caprine placenta. Infection of adult goats resulted in mild clinical signs, however gross lesions observed post mortem were more severe.

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Section: Discussionsupporting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Transplacental infection in goats experimentally infected with a European strain of bluetongue virus serotype 8

Coetzee

Stokstad

Myrmel

et al. 2013

The Veterinary Journal

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“…After the winter season, the viral infection showed a strong recrudescence and dramatically extended its European distribution in 2007, leading to 40 The evolution of BTV infection in Europe is threatening especially when considering the speed and extent of spreading of several serotypes that may favor the emergence of new variants by reassortment [85].…”

Section: Epidemiological Situation From 1900 To 2008mentioning

confidence: 99%

“…Sheep inoculated with VP2, either isolated from intact particles or generated by in vitro expression, produced virusneutralizing antibodies and were resistant to challenge with the homologous BTV serotype [40,81]. Two hydrophylic domains in VP2, AA 199-213 (region 1) and AA 321-346 (region 2), contain multiple conformation-dependent neutralization epitopes [17].…”

Section: Humoral Immunity Against Btvmentioning

confidence: 99%

Bluetongue virus: virology, pathogenesis and immunity

et al. 2008

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-Bluetongue (BT) virus, an orbivirus of the Reoviridae family encompassing 24 known serotypes, is transmitted to ruminants via certain species of biting midges (Culicoides spp.) and causes thrombo-hemorrhagic fevers mainly in sheep. During the 20th century, BTV was endemic in sub-tropical regions but in the last ten years, new strains of BTV (serotypes 1, 2, 4, 8, 9, 16) have appeared in Europe leading to a devastating disease in naive sheep and bovine herds (serotype 8). BTV enters into insect cells via the viral inner core VP7 protein and in mammalian cells via the external capsid VP2 haemagglutinin, which is the major determinant of BTV serotype and neutralization. BTV replicates in mononuclear phagocytes and endothelial cells where it induces expression of inflammatory cytokines as well as apoptosis. BTV can remain as nonreplicating entities concealed in erythrocytes for up to five months. Homologous protection against one BTV serotype involves neutralizing antibodies and T cell responses directed to the external VP2 and VP5 proteins, whereas heterologous protection is supported by T cells directed to the NS1 non structural protein and inner core proteins.

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“…Previous reports on the purification and structural protein components of AHSV (Oellerman et al, 1970;Bremer, 1976;Grubman & Lewis, 1992) are all based on procedures published for BTV (Verwoerd, 1969;Verwoerd et al, 1972;Huismans, 1979;Huismans et al, 1987a, b;Mecham et al, 1986). These methods involve I Present address: The Robens Institute, The University of Surrey, Guildford, Surrey GU2 5XH, U.K.…”

Section: Introductionmentioning

confidence: 99%

Purification and properties of virus particles, infectious subviral particles, cores and VP7 crystals of African horsesickness virus serotype 9

Burroughs

Ohara

Smale

et al. 1994

Journal of General Virology

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Methods were developed for the purification, at high yield, of four different particle types of African horsesickness virus serotype 9 (AHSV-9). These products included virus particles purified on CsC1 gradients which contain proteins apparently directly comparable to those ofbluetongue virus (VP1 to VP7); virus particles purified on sucrose gradients which also contain, as a variable component, protein NS2; infectious subviral particles (ISVPs), containing chymotrypsin cleavage products of VP2; and cores, obtained by treating purified ISVPs with 1 M-MgCIz to remove the components of the outer capsid layer (VP5 and VP2 cleavage products). Additional protein bands migrating with apparent Mrs lower than that of VP5 were detected during SDS-PAGE analysis of virus particles. These appear to be conform-

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Isolation of a capsid protein of bluetongue virus that induces a protective immune response in sheep

Cited by 204 publications

References 15 publications

Transplacental infection in goats experimentally infected with a European strain of bluetongue virus serotype 8

Transplacental infection in goats experimentally infected with a European strain of bluetongue virus serotype 8

Bluetongue virus: virology, pathogenesis and immunity

Purification and properties of virus particles, infectious subviral particles, cores and VP7 crystals of African horsesickness virus serotype 9

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