Isolation of a cDNA Encoding a β-1,4-endoglucanase in the Root-Knot Nematode <i>Meloidogyne incognita</i> and Expression Analysis During Plant Parasitism

Rosso, Marie‐Noëlle; Favery, Bruno; Piotte, Christine; Arthaud, Laury; Boer, J.M. de; Hussey, R. S.; Bakker, J.; Baum, Thomas J.; Abad, Pierre

doi:10.1094/mpmi.1999.12.7.585

Cited by 189 publications

(152 citation statements)

References 34 publications

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“…Most studies have focused on cyst and root-knot nematodes, where cellulases (de Meutter et al 1998;Smant et al 1998;Rosso et al 1999;Goellner et al 2000), xylanase (Dautova et al 2001a) (Uehara et al 2001). We have generated and analyzed 1928 ESTs for P. penetrans, significantly increasing the available sequence information for this nematode.…”

Section: Comparison To C Elegans Genes With Rnai Phenotypesmentioning

confidence: 99%

A survey of SL1-spliced transcripts from the root-lesion nematode Pratylenchus penetrans

et al. 2004

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Plant-parasitic nematodes are important and cosmopolitan pathogens of crops. Here, we describe the generation and analysis of 1928 expressed sequence tags (ESTs) of a splice-leader 1 (SL1) library from mixed life stages of the root-lesion nematode Pratylenchus penetrans. The ESTs were grouped into 420 clusters and classified by function using the Gene Ontology (GO) hierarchy and the Kyoto KEGG database. Approximately 80% of all translated clusters show homology to Caenorhabditis elegans proteins, and 37% of the C. elegans gene homologs had confirmed phenotypes as assessed by RNA interference tests. Use of an SL1-PCR approach, while ensuring the cloning of the 5¢ ends of mRNAs, has demonstrated bias toward short transcripts. Putative nematode-specific and Pratylenchusspecific genes were identified, and their implications for nematode control strategies are discussed.

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Section: Comparison To C Elegans Genes With Rnai Phenotypesmentioning

confidence: 99%

A survey of SL1-spliced transcripts from the root-lesion nematode Pratylenchus penetrans

et al. 2004

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“…Cyst nematode intracellular migration in roots and feeding from parasitized roots cells requires degradation of plant cell walls and this is facilitated by in planta secretion of cellulase by these parasites . Additional cellulase genes more recently have been isolated from H. glycines (Yan et al, 2001;Gao et al, 2002a,b) and other plant-parasitic nematodes including Globodera tabacum (Goellner et al, 2000, Heterodera schachtii (de Meutter et al, 2001), M. incognita (Rosso et al, 1999), and Pratylenchus penetrans (Uehara et al, 2001). The expression of cyst nematode cellulases has been detected in juveniles within eggs, in hatched juveniles, in parasitic second-stage juveniles, but only rarely within third-stage juvenile males or females, and not in any subsequent female cyst nematode life stage (Smant et al, 1997;de Boer et al, 1999;Gao et al, 2002a;Goellner et al, 2000).…”

Section: Rs Hussey Et Almentioning

confidence: 99%

Secrets in secretions: genes that control nematode parasitism of plants

Hussey

Davis

Baum

2002

Braz. J. Plant Physiol.

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The most evolutionary advanced adaptations for plant parasitism by nematodes are the products of parasitism genes expressed in their esophageal gland cells and secreted through their stylet into host tissue to control the complex process of parasitism. Molecular analyses of nematode parasitism genes are revealing the complexity of the tools that enable the nematode to attack plants, and the results paint a more elaborate picture of host cellular events under specific control by the parasite than previously hypothesized. Interestingly, the majority of the parasitism genes discovered encodes proteins unique to plant-parasitic nematodes. Identifying the nematode parasitome, i.e., the complete profile of parasitism gene products secreted through the nematode stylet during the parasitic cycle, is the key to understanding the molecular basis of nematode parasitism of plants. Such knowledge will identify vulnerable points in the parasitic process that can be interfered with to achieve nematode control to limit nematode-induced yield losses in crops.Key words: esophageal gland cells, nematode parasitism, parasitome, parasitism genes, secretion.Secredos em secreções: genes tque controlam o parasitismo de nematóides de plantas: As mais avançadas adaptações evolucionárias para o parasitismo de plantas por nematóides são os produtos dos genes de parasitismo expressos nas células da sua glândula esofagial e secretados através do estilete no tecido do hospedeiro, com a finalidade de controlar o complexo processo de parasitismo. Análises moleculares de genes de parasitismo de nematóides têm revelado a complexidade das ferramentas que os permitem atacar as plantas e também obter uma visão mais elaborada do que antes se tinha por hipótese, dos eventos celulares do hospedeiro sob controle específico do parasita. Interessantemente, a maioria dos genes de parasitismo de nematóides descobertos, codificam proteínas específicas para nematóides parasitas de plantas. Identificar o parasitoma do nematóide, isto é, o perfil completo dos produtos dos genes de parasitismo secretados através do estilete durante o ciclo parasítico, é a chave para a compreensão da base molecular do parasitismo de nematóides de plantas. Este conhecimento permitirá identificar os pontos vulneráveis no processo parasítico que podem sofrer interferência, a fim de se controlar o nematóide e limitar as perdas de produção que eles causam em plantas cultivadas.Palavras-chave: células da glândula esofogial, genes de parasitismo, parasitoma, parasitismo de nematóides, secreção.

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“…This stylet is associated with oesophageal glands, which produce secretions containing cellulases. Endogenous cellulase genes have been identified in obligatory plantparasitic nematodes, such as Heterodera, Globodera and Meloidogyne species (Smant et al, 1998;Rosso et al, 1999;Goellner et al, 2000). The proteins encoded by these genes belong to the glycosyl hydrolase family (GHF), which in B. xylophilus are more similar to fungal GHF cellulases, suggesting acquisition by horizontal gene transfer from fungi (Jones et al, 2005;Kikuchi et al, 2007;Xie et al, 2009).…”

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confidence: 99%

Molecular characterization of Portuguese populations of the pinewood nematodeBursaphelenchus xylophilususing cytochromeband cellulase genes

et al. 2012

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Bursaphelenchus xylophilus is the causal agent of pine wilt disease and a worldwide pest with high economic impact. Since its first diagnosis in Portugal in 1999, it has been subjected to quarantine measures with impact on forest health and ecosystem stability, significantly affecting international trade of wood products. The disease was detected in the north and centre of continental Portugal and, since 2008, the whole country has been considered an affected area. Recently, it was detected in Madeira Island. In order to avoid new outbreaks, it has become of major importance to understand the patterns of spread, introduction points and to characterize the new populations from continental Portugal and Madeira Island. Mitochondrial cytochrome b (cytb) and parasitic cellulase gene sequences were used to evaluate the genetic relationships among isolates that could indicate possible origins of the new outbreaks. Portuguese isolates were compared with isolates from USA, China, Japan and South Korea, in order to investigate possible infection pathways and disease spread patterns in Portugal. Phylogenetic trees based on both genes show that Portuguese isolates group with Asian isolates. Isolates from USA are in a separate position in both gene trees. However, the phylogenetic tree based on the cellulase gene sequences shows higher differentiation among Portuguese isolates than that of cytb. These results agree with those previously obtained using inter-simple sequence repeats (ISSR). This was the first study to use cytb and cellulase genes to characterize pinewood nematode (PWN) populations. This study suggests that cellulase is a better marker than cytb to study genetic diversity in B. xylophilus.

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Isolation of a cDNA Encoding a β-1,4-endoglucanase in the Root-Knot Nematode Meloidogyne incognita and Expression Analysis During Plant Parasitism

Cited by 189 publications

References 34 publications

A survey of SL1-spliced transcripts from the root-lesion nematode Pratylenchus penetrans

A survey of SL1-spliced transcripts from the root-lesion nematode Pratylenchus penetrans

Secrets in secretions: genes that control nematode parasitism of plants

Molecular characterization of Portuguese populations of the pinewood nematodeBursaphelenchus xylophilususing cytochromeband cellulase genes

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