Isolation of a cell surface component of Helicobacter pylori that binds H type 2, Lewis(a), and Lewis(b) antigens

Alkout, Abdulhamid M.; Blackwell, C. Caroline; Weir, D. M.; Poxton, Ian R.; Elton, R. A.; Luman, W; Palmer, K R

doi:10.1016/s0016-5085(97)70129-x

Cited by 77 publications

(67 citation statements)

References 2 publications

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“…KATO III cells were routinely maintained in RPMI 1640 medium supplemented with 20% FCS and with gentamicin (20 mg/L) in a humidified incubator containing 5% CO 2 (all Sigma-Aldrich, Munich, Germany). KATO III cells were chosen because subclones of this cell line were previously described to express Le b receptors (29). We confirmed the presence of this epitope on KATO III cells by immunocytochemistry.…”

Section: Coculture Of H Pylori With Kato III Cells and Determinationmentioning

confidence: 59%

TheHelicobacter pyloriBlood Group Antigen-Binding Adhesin Facilitates Bacterial Colonization and Augments a Nonspecific Immune Response

Rad¹,

Gerhard²,

Lang

et al. 2002

The Journal of Immunology

162

104

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Presence of the Helicobacter pylori adherence factor blood group Ag-binding adhesin (BabA; binding to Lewisb (Leb)) is associated with ulcer disease, adenocarcinoma, and precancerous lesions. The importance of BabA for bacterial colonization and the inflammatory response is unknown. A total of 141 antral biopsies from H. pylori-infected patients were assessed in regard to the degree of granulocytic (G0°–G3°) and lymphocytic (L1°–L3°) infiltration. DNA genotypes of babA2 (the transcriptionally active gene of BabA), cagA, and vacAs1/2 were determined by PCR. Colonization density and Leb status on gastric epithelial cells were determined by immunohistochemistry. Real-time quantitative (TaqMan) RT-PCR determined mRNA expression of IL-8, TNF -α, and the Th1 markers IFN-γ and the IL-12R β2 chain. A total of 91% of infected patients were Leb positive. The vacAs1+/cagA+ strains harboring babA2 showed significantly higher levels of granulocytic infiltration, bacterial colonization, and IL-8 mRNA than vacAs1+/cagA+ strains lacking babA2. IL-8 mRNA and protein production by KATO III cells in vitro increased dose dependently with addition of different numbers of type 1 strains (G27 and 2808 strains, 0.1–20 bacteria/cell). The mRNA expression of TNF-α, IFN-γ, and IL-12R β2 was higher in H. pylori-positive patients than in controls, but it did not differ significantly between patients infected with different strain types. These data suggest that BabA facilitates colonization of H. pylori and thereby increases IL-8 response, resulting in enhanced mucosal inflammation. Infection with strains harboring BabA thereby augment a nonspecific immune response, whereas the Th1 response toward H. pylori appears to be independent of BabA, cytotoxin-associated gene A, or vacuolating cytotoxin.

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Section: Coculture Of H Pylori With Kato III Cells and Determinationmentioning

confidence: 59%

TheHelicobacter pyloriBlood Group Antigen-Binding Adhesin Facilitates Bacterial Colonization and Augments a Nonspecific Immune Response

Rad¹,

Gerhard²,

Lang

et al. 2002

The Journal of Immunology

162

104

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“…pylori key-adhesins for competing H type 2, Lewis-a and Lewis-b antigenic receptors in gastric mucosa [69,70] . Proactive role of some seemingly unrelated factors such as leptin resistance, trypsin inhibitor types, passage into blood plasma of intestinal alkaline phosphatase, PTC tasting ability etc., which have a bearing on the etiology of peptic ulcers also need to be investigated.…”

Section: Resultsmentioning

confidence: 99%

“…Clyne and Drumm [68] , using flow cytometry to investigate the binding of H. pylori to Kato III cells and the primary gastric epithelial cells, found that adherence of the bacterium occurred in a manner independent of Lewis-a and Lewis-b expression. Alkout and coworkers [69] identified an H. pylori adhesin 61-kDa that binds H type 2, Lewis-a and Lewis-b antigens. They argued that if a key adhesin of H. pylori binds to H type 2 of epithelial cells, the presence of Lewis-b in the mucus of secretors will compete with greater effect for the 61-kDa adhesin and reduce colonisation of H. pylori.…”

Section: Hypothesismentioning

confidence: 99%

Revisiting D'Adamo's Blood Type Diet: The Critical Role of Secreted Antigens in Digestive Health - An Evolutionary Perspective

Bhalla¹

2017

jmscr

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HYPOTHESISThe ability to secrete A-B-H substances in saliva and other glandular secretions was, perhaps, of paramount adaptive significance at a stage in the early history of man when human ancestors subsisted on raw food like other animals. Dietary habits shifted profoundly with the invention of fire making techniques, which greatly increased consumption of cooked food, The cultural revolution in food habits of early man resulted in relaxation of selection pressure on the dominant secretor gene (Se) necessary for survival in a lectin-rich dietary environment. With the abatement of selection pressure, conditions were set for the recessive mutant gene 'se', to accumulate over a long period of time, leading to the establishment of ABH polymorphism in man. Evidence lending support to 'selection-relaxation' hypothesis is derived from the distribution of secretor types (which is purportedly related to history of dependence on raw food) in man and non-human primates. Serological investigations carried out on hundreds of apes and monkeys reveal that they are invariably secretors. The nonwww.jmscr.igmpublication.org Impact Factor 5.84 Index Copernicus Value: 83.27

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“…As a biological reagent, MAb L9E10 may be useful in the study of cancer biology, particularly cancer stem cell biology as well as gastric diseases caused by H. pylori bacteria, with attachment to human gastric epithelial cells mediated by blood group antigens Lewis b and H2. (31,32) Additionally, it can be used for the identification of the Bombay blood type in which erythrocytes have no BG-H2 antigen expression. (28) …”

Section: Resultsmentioning

confidence: 99%

MAb L9E10 to Blood Group H2 Antigen Binds to Colon Cancer Stem Cells and Inhibits Tumor Cell Migration and Invasion

Wang

Gildersleeve

et al. 2010

Hybridoma

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The functions of the precursor H antigen for ABO blood group antigens are still not fully understood, particularly in cancer cells. In this study, we used hybridoma technology and NSY human colon cancer cells as an immunogen to generate a monoclonal antibody designated as MAb L9E10. The binding antigen of MAb L9E10 was identified as blood group (BG) H2 antigen using carbohydrate array and erythrocyte agglutination assays. In immunofluorescence study, we found that BG-H2 was expressed on the surfaces of both colon cancer stem cells and their differentiated progeny. In a functional study, we observed that MAb L9E10 inhibited tumor cell migration and invasion at a concentration of 10 mg/mL in vitro. This result suggests that MAb L9E10 could be used to study cancer biology, particularly cancer stem cell biology. In addition, it is potentially useful for studying gastric diseases caused by Helicobacter pylori bacteria, with attachment to human gastric epithelial cells mediated by blood group antigens Lewis b and H2. Finally, MAb L9E10 is an ideal biological reagent for identifying Bombay blood type in which erythrocytes have no BG-H2 antigen expression.

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Isolation of a cell surface component of Helicobacter pylori that binds H type 2, Lewis(a), and Lewis(b) antigens

Cited by 77 publications

References 2 publications

TheHelicobacter pyloriBlood Group Antigen-Binding Adhesin Facilitates Bacterial Colonization and Augments a Nonspecific Immune Response

TheHelicobacter pyloriBlood Group Antigen-Binding Adhesin Facilitates Bacterial Colonization and Augments a Nonspecific Immune Response

Revisiting D'Adamo's Blood Type Diet: The Critical Role of Secreted Antigens in Digestive Health - An Evolutionary Perspective

MAb L9E10 to Blood Group H2 Antigen Binds to Colon Cancer Stem Cells and Inhibits Tumor Cell Migration and Invasion

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