Isolation of a Cellulolytic Enzyme Producing Microorganism, Culture Conditions and Some Properties of the Enzymes

Yamanobe, Takashi; Mitsuishi, Yasushi; Takasaki, Yoshiyuki

doi:10.1080/00021369.1987.10867998

Cited by 16 publications

(22 citation statements)

References 3 publications

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“…Natsukaze) [22] when used as a silage additive. In addition to endoglucanase activity, ENZ has greater β-glucosidase activity than some other products such as those derived from Trichoderma viride [23]. The linear increase in in vitro DMD of dry-rolled barley with increasing ENZ addition observed in Experiment 2 was confirmed in Experiment 3, and was consistent with the observed increase in total gas production (trend only in Experiment 2) and decrease in fermentation pH in both experiments.…”

Section: Discussionsupporting

confidence: 74%

Effects of particle size of processed barley grain, enzyme addition and microwave treatment on in vitro disappearance and gas production for feedlot cattle

Tagawa

Holtshausen

McAllister

et al. 2016

Asian-Australas J Anim Sci

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ObjectiveThe effects of particle size of processed barley grain, enzyme addition and microwave treatment on in vitro dry matter (DM) disappearance (DMD), gas production and fermentation pH were investigated for feedlot cattle.MethodsRumen fluid from four fistulated feedlot cattle fed a diet of 860 dry-rolled barley grain, 90 maize silage and 50 supplement g/kg DM was used as inoculum in 3 batch culture in vitro studies. In Experiment 1, dry-rolled barley and barley ground through a 1-, 2-, or 4-mm screen were used to obtain four substrates differing in particle size. In Experiment 2, cellulase enzyme (ENZ) from Acremonium cellulolyticus Y-94 was added to dry-rolled and ground barley (2-mm) at 0, 0.1, 0.5, 1, and 2 mg/g, while Experiment 3 examined the interactions between microwaving (0, 30, and 60 s microwaving) and ENZ addition (0, 1, and 2 mg/g) using dry-rolled barley and 2-mm ground barley.ResultsIn Experiment 1, decreasing particle size increased DMD and gas production, and decreased fermentation pH (p<0.01). The DMD (g/kg DM) of the dry-rolled barley after 24 h incubation was considerably lower (p<0.05) than that of the ground barley (119.1 dry-rolled barley versus 284.8 for 4-mm, 341.7 for 2-mm; and 358.6 for 1-mm). In Experiment 2, addition of ENZ to dry-rolled barley increased DMD (p<0.01) and tended to increase (p = 0.09) gas production and decreased (p<0.01) fermentation pH, but these variables were not affected by ENZ addition to ground barley. In Experiment 3, there were no interactions between microwaving and ENZ addition after microwaving for any of the variables. Microwaving had minimal effects (except decreased fermentation pH), but consistent with Experiment 2, ENZ addition increased (p<0.01) DMD and gas production, and decreased (p<0.05) fermentation pH of dry-rolled barley, but not ground barley.ConclusionWe conclude that cellulase enzymes can be used to increase the rumen disappearance of barley grain when it is coarsely processed as in the case of dry-rolled barley. However, microwaving of barley grain offered no further improvements in ruminal fermentation of barley grain.

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Section: Discussionsupporting

confidence: 74%

Effects of particle size of processed barley grain, enzyme addition and microwave treatment on in vitro disappearance and gas production for feedlot cattle

Tagawa

Holtshausen

McAllister

et al. 2016

Asian-Australas J Anim Sci

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“…Various nitrogen sources were proved to have different influences on carbohydrate degradation [28][29][30][31][32][33][34][35]. In this study, the presence of yeast extract, tryptone, or peptone in the culture medium enhanced cellulase activities more compared with inorganic nitrogen sources as shown in Table 1.…”

Section: Medium Optimizationmentioning

confidence: 88%

Optimization of Growth Medium and Enzyme Assay Conditions for Crude Cellulases Produced by a Novel Thermophilic and Cellulolytic Bacterium, Anoxybacillus sp. 527

Liang

Feng

Yesuf

et al. 2009

Appl Biochem Biotechnol

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A newly isolated Anoxybacillus sp. 527 was found to grow on crystalline cellulose as sole carbon and energy sources. Cellulases secreted by strain 527 were better induced by cellobiose, followed by glucose, lactose, sucrose, and cellulose. Cellulase secretion was enhanced by an optimized medium. Cellulase activity was increased by the addition of Ca(2+) and NH (4) (+) and achieved maximum as 7.0 FPU ml(-1) at 70 degrees C and pH 6.0. Even at 100 degrees C, the enzymes were still active, which implies their potential application in large-scale cellulose conversion process.

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“…This ability is influenced by the composition of the enzyme components, particularly crystalline cellulose‐hydrolyzing enzyme (FPase) and β‐glucosidase. In contrast, Acremonium cellulolyticus , which was isolated in 1987 and developed to increase performance, is able to produce both enzymes, besides carbomethyl cellulose‐hydrolyzing enzyme (CMCase) and a small amount of xylanase, β‐1,3‐glucanase, and amylase (4).…”

Section: Introductionmentioning

confidence: 99%

“…Indeed, the commercial enzymes produced from A. cellulolyticus have a high hydrolyzing performance with regards to cellulolytic wastes, compared to other Trichoderma enzymes (6). Despite its high enzymatic performance, only a few studies on A. cellulolyticus for cellulase production have been reported (4 − 9). In particular, the results obtained in the studies (4) have cast light on its unique enzyme composition and its relatively high cellulase activity.…”

Section: Introductionmentioning

confidence: 99%

Efficient Cellulase Production by the Filamentous Fungus Acremonium cellulolyticus

Ikeda

Hayashi

Okuda³

et al. 2007

Biotechnology Progress

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Cellulase production was investigated in a culture of a strain of Acremonium cellulolyticus. The medium components were optimized for the improvement of cellulase production. The maximum production of cellulolytic enzymes was obtained in a medium containing (grams per liter) 50 Solka Floc, 5 (NH4)2SO4, 24 KH2PO4, 4.7 potassium tartrate hemihydrate, 1.2 MgSO4.7H2O, 1 Tween 80, 4 urea, 0.01 ZnSO4.7H2O, 0.01 MnSO4.6H2O, and 0.01 CuSO4.7H2O, with a pH of 4.0. In the flask culture, 15.5 filter paper units (FPU)/mL of maximum cellulase activity was obtained, 17.42 FPU/mL in a 7-L bioreactor, and 13.08 FPU/mL in a 50-L scale bioreactor for 4-8 d at 30 degrees C. Average production rates were 1.94 FPU/mL.d in flasks, 2.86 FPU/mL.d in the 7-L bioreactor, and 2.56 FPU/mL.d in the 50-L bioreactor. Cellulase production on a small scale was successfully reproduced in the 50-L pilot scale bioreactor. Saccharification activity from A. cellulolyticus was compared with cellulolytic enzymes produced by other strains. The A. cellulolyticus culture broth had a comparable saccharification yield in comparison with those of other Trichoderma enzymes (GC220 or Cellulosin T2) under the same total cellulase activity. Its saccharification yield (percent of released reducing sugar to used dried substrate) was 60%, and its glucose content was 83%.

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Isolation of a Cellulolytic Enzyme Producing Microorganism, Culture Conditions and Some Properties of the Enzymes

Cited by 16 publications

References 3 publications

Effects of particle size of processed barley grain, enzyme addition and microwave treatment on in vitro disappearance and gas production for feedlot cattle

Effects of particle size of processed barley grain, enzyme addition and microwave treatment on in vitro disappearance and gas production for feedlot cattle

Optimization of Growth Medium and Enzyme Assay Conditions for Crude Cellulases Produced by a Novel Thermophilic and Cellulolytic Bacterium, Anoxybacillus sp. 527

Efficient Cellulase Production by the Filamentous Fungus Acremonium cellulolyticus

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