Isolation of a Collagenolytic Gram Negative Yellow Pigmented Bacterium

Towards and Understanding of the Microbial Decomposition of Archaeological Bone in the Burial Environment

“…The plates were incubated at 10 C for 14 days, some of the growth removed and the agar examined for clearing. Those isolates which cleared the agar were designated presumptively positive for the production of a collagenase (Labadie, 1982).…”

“…In the collagen, tryptone soya and fungal media, the cultures were set up in quadruplicate and kanamycin added at a concentration of 39·15 g cm 3 to two of each. Subcultures were made to collagen plates (Labadie, 1982) and fresh media after 7 days of incubation, and the subcultures were made three times; therefore at each subculture the amount of original material present was diluted. The agar plates were incubated for 14 days at 10 C.…”

Involvement of the collagenase produced by Corynebacterium rathayii in its adhesion to collagen

“…The bacterium used in this study was a yellow pigmented bacterium previously identified as Empedobacter collagenolyticum [8]. Recent classification of this microorganism by DNA-DNA hybridization showed that it is in fact a phytopathogenic bacterium, Corynebacterium rathayii [9].…”