Isolation of a dimethylsulfide-utilizingHyphomicrobium species and its application in biofiltration of polluted air

Pol, Arjan; Camp, Huub J. M. Op den; Mees, S. G. M.; Kersten, M.; Drift, Chris van der

doi:10.1007/bf00700635

Cited by 76 publications

(47 citation statements)

References 27 publications

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“…Results revealed that only Hyphomicrobium strains were able to grow using the three substrates individually as the only carbon and energy source, similar to results of previous studies (De Bont et al 1981;Pol et al 1994). The other isolates grew on methanol as their sole carbon and energy source, but were unable to grow on DMS and DMSO.…”

Section: Methylotrophic Bacteria Isolated From the Enrichment Culturessupporting

confidence: 88%

Culture-dependent and culture-independent methods reveal diverse methylotrophic communities in terrestrial environments

Eyice

Schäfer

2015

Arch Microbiol

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Original citation:Eyice, Özge and Schäfer, Hendrik. (2015) Culture-dependent and culture-independent methods reveal diverse methylotrophic communities in terrestrial environments. Archives of Microbiology. Permanent WRAP url:http://wrap.warwick.ac.uk/73935 Copyright and reuse:The Warwick Research Archive Portal (WRAP) makes this work by researchers of the University of Warwick available open access under the following conditions. Copyright © and all moral rights to the version of the paper presented here belong to the individual author(s) and/or other copyright owners. To the extent reasonable and practicable the material made available in WRAP has been checked for eligibility before being made available.Copies of full items can be used for personal research or study, educational, or not-for profit purposes without prior permission or charge. Provided that the authors, title and full bibliographic details are credited, a hyperlink and/or URL is given for the original metadata page and the content is not changed in any way. Publisher's statement:The final publication is available at Springer via http://dx.doi.org/10.1007/s00203-015-1160-x A note on versions: The version presented here may differ from the published version or, version of record, if you wish to cite this item you are advised to consult the publisher's version. Please see the 'permanent WRAP url' above for details on accessing the published version and note that access may require a subscription. They are suggested to affect atmospheric chemistry and global climate. Methylotrophic microorganisms are considered as a significant sink for these compounds, therefore we analysed the diversity of terrestrial bacteria that utilise methanol, DMS and DMSO as carbon and energy source using culture-dependent and -independent methods. The effect of habitat type on the methylotrophic community structure was also investigated in rhizosphere and bulk soil. While thirteen strains affiliated to the genera

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Section: Methylotrophic Bacteria Isolated From the Enrichment Culturessupporting

confidence: 88%

Culture-dependent and culture-independent methods reveal diverse methylotrophic communities in terrestrial environments

Eyice

Schäfer

2015

Arch Microbiol

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“…Both aerobic and anaerobic DMS-degrading microorganisms have been previously described. Aerobic DMS-degrading microorganisms that have been previously isolated from sewage treatment plants, marine sediments, soil and biofilters include Thiobacilli (De Zwart and Kuenen, 1992;Visscher and Taylor, 1993), Hyphomicrobia (Suylen and Kuenen, 1986;Pol et al, 1994) and Methylophaga (De Zwart et al, 1996). Anaerobic DMS-degrading bacteria previously described are methanogens (Kiene et al, 1986;Lomans et al, 1999b), anoxygenic phototrophs (Widdel and Pfennig, 1981;Zeyer et al, 1987), sulfate-reducing bacteria (Tanimoto and Bak, 1994) and denitrifying bacteria (Visscher and Taylor 1993b).…”

Section: Oxidation Of Reduced Sulfur Compoundsmentioning

confidence: 99%

Bacteria associated with iron seeps in a sulfur-rich, neutral pH, freshwater ecosystem

et al. 2008

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The freshwater nature reserve De Bruuk is an iron-and sulfur-rich minerotrophic peatland containing many iron seeps and forms a suitable habitat for iron and sulfur cycle bacteria. Analysis of 16S rRNA gene-based clone libraries showed a striking correlation of the bacterial population of samples from this freshwater ecosystem with the processes of iron reduction (genus Geobacter), iron oxidation (genera Leptothrix and Gallionella) and sulfur oxidation (genus Sulfuricurvum).Results from fluorescence in situ hybridization analyses with a probe specific for the beta-1 subgroup of Proteobacteria, to which the genera Leptothrix and Gallionella belong, and newly developed probes specific for the genera Geobacter and Sulfuricurvum, supported the clone library data. Molecular data suggested members of the epsilonproteobacterial genus Sulfuricurvum as contributors to the oxidation of reduced sulfur compounds in the iron seeps of De Bruuk. In an evaluation of anaerobic dimethyl sulfide (DMS)-degrading activity of sediment, incubations with the electron acceptors sulfate, ferric iron and nitrate were performed. The fastest conversion of DMS was observed with nitrate. Further, a DMS-oxidizing, nitrate-reducing enrichment culture was established with sediment material from De Bruuk. This culture was dominated by dimorphic, prosthecate bacteria, and the 16S rRNA gene sequence obtained from this enrichment was closely affiliated with Hyphomicrobium facile, which indicates that the Hyphomicrobium species are capable of both aerobic and nitrate-driven DMS degradation.

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“…These bacteria are ubiquitous in the biosphere and have been isolated from a wide variety of natural habitats including plants, soils and freshwater and marine sediments (Anthony, 1982). Methylotrophs play an important role in biogeochemical cycling and possess a potential for use in bioremediation (Pol et al, 1994;De Marco et al, 2004). We are studying a specific habitat that is rich in methylotrophic activity: the sediment of Lake Washington at a depth of about 60 m (Kuivila et al, 1988;Costello & Lidstrom, 1999).…”

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confidence: 99%

Labrys methylaminiphilus sp. nov., a novel facultatively methylotrophic bacterium from a freshwater lake sediment

Miller

Kalyuzhnaya

Noyes

et al. 2005

International Journal of Systematic and Evolutionary Microbiology

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A new bacterial isolate from a methylamine enrichment culture is described, representing a novel species of facultatively methylotrophic bacteria. The non-motile bacterium is Gram-negative, replicates by budding and does not form endospores. The isolate utilizes methylated amines, as well as a variety of monosaccharides, disaccharides, amino acids, organic acids, aromatic compounds and alcohols as substrates, but does not utilize methanol. Growth factors are not required, although yeast extract stimulates growth. The major components of the fatty acid profile are C 18 : 1 v7c, C 19 : 0 cyclo and C 16 : 0 . The dominant cellular phospholipids are phosphatidyl acid, phosphatidylcholine and phosphatidylethanolamine. The G+C content of the DNA is 65?7±0?3 mol%. 16S rRNA gene-based phylogenetic analysis revealed that the novel isolate belongs to the a-Proteobacteria and is closely related to the only representative of the genus Labrys, Labrys monachus (97?4 % sequence similarity). However, the level of DNA-DNA relatedness with L. monachus is less than 3 %, justifying the placement of this isolate into a novel species of the genus Labrys. The name Labrys methylaminiphilus sp. nov. is proposed (type strain JLW10 T =ATCC BAA-1080 T =DSM 16812 T ).Methylotrophs are a group of bacteria capable of metabolizing compounds containing no carbon-carbon bonds as sole sources of carbon and energy. These bacteria are ubiquitous in the biosphere and have been isolated from a wide variety of natural habitats including plants, soils and freshwater and marine sediments (Anthony, 1982). Methylotrophs play an important role in biogeochemical cycling and possess a potential for use in bioremediation (Pol et al., 1994; De Marco et al., 2004). We are studying a specific habitat that is rich in methylotrophic activity: the sediment of Lake Washington at a depth of about 60 m (Kuivila et al., 1988;Costello & Lidstrom, 1999). Until recently, research at this site has focused primarily on methanotroph populations (Auman et al., 2000;Costello & Lidstrom, 1999) and no survey of broader methylotroph presence has been conducted. This study focused on enrichment, isolation and taxonomic description of methylamine-utilizing bacteria from Lake Washington sediment. One of these isolates, a novel facultative methylotroph, is described. Sediment samples were collected on 21 July 2003, off the RV Clifford Barnes from a 63 m-deep site in Lake Washington, Seattle, WA, USA (47 u 38?0759 N 122 u 15?9939 W) using a box core that allowed collection of undisturbed sediment. Sediment cores were taken to a depth of approximately 20 cm and subsectioned into layers approximately 1?5 cm thick. Samples were transported to the laboratory on ice and used immediately. One millilitre samples of the upper 1?0 cm of the sediment cores were inoculated into 250 ml flasks containing 50 ml 0?26 salts medium (Harder et al., 1973) supplemented with 0?01 % methylamine (w/v) and were incubated for 6-8 days at room temperature with shaking (125 r.p.m.). In subsequent enrichments, ...

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Isolation of a dimethylsulfide-utilizingHyphomicrobium species and its application in biofiltration of polluted air

Cited by 76 publications

References 27 publications

Culture-dependent and culture-independent methods reveal diverse methylotrophic communities in terrestrial environments

Culture-dependent and culture-independent methods reveal diverse methylotrophic communities in terrestrial environments

Bacteria associated with iron seeps in a sulfur-rich, neutral pH, freshwater ecosystem

Labrys methylaminiphilus sp. nov., a novel facultatively methylotrophic bacterium from a freshwater lake sediment

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