2007
DOI: 10.1002/jobm.200610263
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Isolation of a FAD‐GPDH gene encoding a mitochondrial FAD‐dependent glycerol‐3‐phosphate dehydrogenase from Dunaliella salina

Abstract: The mitochondrial FAD-dependent glycerol-3-phosphate dehydrogenase (FAD-GPDH), recently reported in plants, has been detailed in yeast and animal systems. It oxidizes glycerol-3-phosphate (G-3-P) to dihydroxyacetone phosphate (DHAP) on the outer surface of mitochondrial inner membrane. A cDNA encoding the Dunaliella salina mitochondrial glycerol-3-phosphate dehydrogenase (DsFAD-GPDH) has been cloned and sequenced. The full length cDNA is 2791 bp, with an open reading frame (ORF) encoding 650 predicted amino ac… Show more

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Cited by 15 publications
(12 citation statements)
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“…This indicates that the, up-regulation of DsPFK expression occurs in order to satisfy the requirement of the massive synthesis of glycerol under hyperosmotic stress. The response of DsPFK to hyperosmotic stress is similar to that of DsFAD-GPDH and DsGPI [9,25]. Transcription levels first increased significantly and then decreased significantly, suggesting that the functions of DsPFK, DsFAD-GPDH and DsGPI are coordinated.…”
Section: Discussionmentioning
confidence: 72%
See 1 more Smart Citation
“…This indicates that the, up-regulation of DsPFK expression occurs in order to satisfy the requirement of the massive synthesis of glycerol under hyperosmotic stress. The response of DsPFK to hyperosmotic stress is similar to that of DsFAD-GPDH and DsGPI [9,25]. Transcription levels first increased significantly and then decreased significantly, suggesting that the functions of DsPFK, DsFAD-GPDH and DsGPI are coordinated.…”
Section: Discussionmentioning
confidence: 72%
“…The content of intracellular dihydroxyacetone phosphate determines the rate and amount of the production of glycerol. Phosphofructokinase (PFK) [8] and glycerol-3-phosphate dehydrogenase (GPD) [9][10][11] are two key enzymes of this process. PFK catalyzes the conversion of fructose-6-phosphate to fructose-1,6-bisphosphate [12,13], and has the characteristics of allosteric modulation in bacteria, plants, and animals, keeping consistent with the activity of glycolysis [14].…”
Section: Introductionmentioning
confidence: 99%
“…-GPDHs in Saccharomyces cerevisiae and Arabidopsis combine their activity with a mitochondrial FAD-dependent GPDH to form the G3P shuttle, which function seems to be transferring reducing equivalents from cytosol into the mitochondrial respiratory chain [19,29,31]. In microalgae, a mitochondrial FAD-GPDH has recently been identified for D. salina [41]. It will be interesting to investigate whether CrGPDH1 could be involved in a similar G3P shuttle in C. reinhardtii.…”
Section: Discussionmentioning
confidence: 97%
“…; Yang et al . ). Both the transcription and enzyme activities of FAD‐dependent GPDH were shown to increase after 1–3 h but declined after 3–6 h of salt stress (Yang et al .…”
Section: Introductionmentioning
confidence: 97%