Isolation of a Flavone Apigenin and a Steroids Squalene from Peronema canescens Jack Leaves with Anti-Inflammatory Activities

Abstract

Research on the isolation of sungkai leaf bioactive compounds has not been explored in depth as an anti-inflammatory, in this study the aim was to partition the ethanol extract into n-hexane fraction and ethanol fraction as well as to test its antiinflammatory activity with male white mice using the method of forming air sacs and artificial edema on the backs of mice. with carrageenan administered subcutaneously. In addition, isolates with good antiinflammatory activity were characterized using UV-Vis and IR spectrophotometry.

Determination of Flavonoid Content and Anti-Inflammatory Activity Extract and Fraction of Sungkai Leaf (Peronema canescens Jack)

“…The acetone : ethanol (6:4) mobile phase was then used to elute the samples. Following the elution procedure, measure the retention factor value for each colour fluorescence and use a 254 nm and 366 nm UV lamp to observe the stain's form (19) .…”

“…The choice of eluent is based on the ability of the eluent to elute compounds. The eluent used in this TLC test was acetone : ethanol (6:4), based on research by Ardhica (19), which used the same plant extracts and eluent. According to Ardhica, the Sungkai leaf's pure ethanol extract contained flavonoid compounds with an RF value of 0.71 (19) .…”

“…The eluent used in this TLC test was acetone : ethanol (6:4), based on research by Ardhica (19), which used the same plant extracts and eluent. According to Ardhica, the Sungkai leaf's pure ethanol extract contained flavonoid compounds with an RF value of 0.71 (19) . The results of the TLC test in Table 4 show the similarity of the RF values with Ardhica's research, where the RF values of the ethanol extract and ethanol fraction were 0.75 and 0.72 and gave a blue glow in the 366 nm UV lamp; however, the colour fluorescence was still not wholly separated.…”

Formulation and Characterization of a Microencapsulant of Sungkai Leaves Ethanol Extract (<em>Peronema canescens</em> Jack)

“…This plant is traditionally used by the community in medicine or health care, such as bruising medicine, fever medicine, cold medicine, worming medicine, and mouthwash [2]. P. canescens contains bioactive compounds that play as antidiaberics [3], antihyperurisemia [4], anti-inflammatory [5], potential anticancer [6], and immunostimulant [7]. Other studies mention that P. canescens has potential bioactivity as an antibacterial and antioxidant.…”

“…Other studies mention that P. canescens has potential bioactivity as an antibacterial and antioxidant. This potential is related to the content of secondary metabolite compounds possessed by P. canescens plants, such as alkaloids, terpenoids, phenolics, flavonoids, tannins, and saponins [5].…”