Isolation of a galactose-binding lectin from the venom of the snake Bothrops godmani (Godmann's pit viper)

Lomonte, Bruno; Rojas, Gustavo; Gutiérrez, José Marı́a; Ramirez, G.

doi:10.1016/0041-0101(90)90008-u

Cited by 32 publications

(16 citation statements)

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“…These Ca 2+ -dependent lectins induce platelet aggregation [9,120] and are potent mitogens [121,122]. In all previous studies, GBLs have been found at very low levels [120], and their known sequences display very little sequence variation [10].…”

Section: Resultsmentioning

confidence: 99%

“…However, with the present findings, that view may have to be revised somewhat. While several species in the present study also have typically low GBL titers (0–0.19%)(Table S2), M. paraensis (1.93%) and M. corallinus (3.35%) have the highest levels reported in any snake venom to date, suggesting that in these two species, GBL hemagglutinating and edematogenic properties [122] may play a significant role in envenomation. Signal peptides are shown to the left of the vertical black line.…”

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confidence: 78%

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Coralsnake Venomics: Analyses of Venom Gland Transcriptomes and Proteomes of Six Brazilian Taxa

Aird

Silva

Qiu

et al. 2017

Toxins

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Venom gland transcriptomes and proteomes of six Micrurus taxa (M. corallinus, M. lemniscatus carvalhoi, M. lemniscatus lemniscatus, M. paraensis, M. spixii spixii, and M. surinamensis) were investigated, providing the most comprehensive, quantitative data on Micrurus venom composition to date, and more than tripling the number of Micrurus venom protein sequences previously available. The six venomes differ dramatically. All are dominated by 2–6 toxin classes that account for 91–99% of the toxin transcripts. The M. s. spixii venome is compositionally the simplest. In it, three-finger toxins (3FTxs) and phospholipases A2 (PLA2s) comprise >99% of the toxin transcripts, which include only four additional toxin families at levels ≥0.1%. Micrurus l. lemniscatus venom is the most complex, with at least 17 toxin families. However, in each venome, multiple structural subclasses of 3FTXs and PLA2s are present. These almost certainly differ in pharmacology as well. All venoms also contain phospholipase B and vascular endothelial growth factors. Minor components (0.1–2.0%) are found in all venoms except that of M. s. spixii. Other toxin families are present in all six venoms at trace levels (<0.005%). Minor and trace venom components differ in each venom. Numerous novel toxin chemistries include 3FTxs with previously unknown 8- and 10-cysteine arrangements, resulting in new 3D structures and target specificities. 9-cysteine toxins raise the possibility of covalent, homodimeric 3FTxs or heterodimeric toxins with unknown pharmacologies. Probable muscarinic sequences may be reptile-specific homologs that promote hypotension via vascular mAChRs. The first complete sequences are presented for 3FTxs putatively responsible for liberating glutamate from rat brain synaptosomes. Micrurus C-type lectin-like proteins may have 6–9 cysteine residues and may be monomers, or homo- or heterodimers of unknown pharmacology. Novel KSPIs, 3× longer than any seen previously, appear to have arisen in three species by gene duplication and fusion. Four species have transcripts homologous to the nociceptive toxin, (MitTx) α-subunit, but all six species had homologs to the β-subunit. The first non-neurotoxic, non-catalytic elapid phospholipase A2s are reported. All are probably myonecrotic. Phylogenetic analysis indicates that the six taxa diverged 15–35 million years ago and that they split from their last common ancestor with Old World elapines nearly 55 million years ago. Given their early diversification, many cryptic micrurine taxa are anticipated.

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 78%

Coralsnake Venomics: Analyses of Venom Gland Transcriptomes and Proteomes of Six Brazilian Taxa

Aird

Silva

Qiu

et al. 2017

Toxins

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“…Ogilvie et al [119] likewise found low expression levels for GBLs in Bothrops atrox (0.2%) and Dendroaspis jamesonii (0.4%) venoms, with a somewhat higher level (0.8%) in Lachesis muta venom. Lomonte et al [120] found that the GBL from Cerrophidion godmani venom exhibited edema-forming activity in mice, but concluded that with its low potency and low abundance, it probably plays relatively little role in envenomation. The aforementioned data suggest that GBLs may exist in venom as mitogens to regulate synthetic activity in the glandular epithelium itself.…”

Section: Resultsmentioning

confidence: 99%

Quantitative high-throughput profiling of snake venom gland transcriptomes and proteomes (Ovophis okinavensis and Protobothrops flavoviridis)

et al. 2013

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BackgroundAdvances in DNA sequencing and proteomics have facilitated quantitative comparisons of snake venom composition. Most studies have employed one approach or the other. Here, both Illumina cDNA sequencing and LC/MS were used to compare the transcriptomes and proteomes of two pit vipers, Protobothrops flavoviridis and Ovophis okinavensis, which differ greatly in their biology.ResultsSequencing of venom gland cDNA produced 104,830 transcripts. The Protobothrops transcriptome contained transcripts for 103 venom-related proteins, while the Ovophis transcriptome contained 95. In both, transcript abundances spanned six orders of magnitude. Mass spectrometry identified peptides from 100% of transcripts that occurred at higher than contaminant (e.g. human keratin) levels, including a number of proteins never before sequenced from snakes. These transcriptomes reveal fundamentally different envenomation strategies. Adult Protobothrops venom promotes hemorrhage, hypotension, incoagulable blood, and prey digestion, consistent with mammalian predation. Ovophis venom composition is less readily interpreted, owing to insufficient pharmacological data for venom serine and metalloproteases, which comprise more than 97.3% of Ovophis transcripts, but only 38.0% of Protobothrops transcripts. Ovophis venom apparently represents a hybrid strategy optimized for frogs and small mammals.ConclusionsThis study illustrates the power of cDNA sequencing combined with MS profiling. The former quantifies transcript composition, allowing detection of novel proteins, but cannot indicate which proteins are actually secreted, as does MS. We show, for the first time, that transcript and peptide abundances are correlated. This means that MS can be used for quantitative, non-invasive venom profiling, which will be beneficial for studies of endangered species.

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“…Snake venom lectins are able to inhibit or activate specific platelet membrane receptors and blood coagulation factors (Morita, 2004(Morita, , 2005Ogawa et al, 2005;Wang, 2008) and can promote a diversity of biological effects, such as lymphocyte proliferation (Mastro et al, 1986), induction of edema (Lomonte et al, 1990;Panunto et al, 2006), induction of Ca 2+ release from the sarcoplasmic reticulum (Ohkura et al, 1996), inhibition of cancer cell proliferation (Pereira-Bittencourt et al, 1999), erythrocyte agglutination in vitro (Kassab et al, 2001), cytotoxicity to tumors and endothelial cell lines (Carvalho et al, 2001), renal effects (Havt et al, 2005) and induction of rolling of leukocytes (Elífio-Esposito et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Purification of a lectin with antibacterial activity from Bothrops leucurus snake venom

Nunes

Souza

Vaz

et al. 2011

Comparative Biochemistry and Physiology Part B: Biochemistry an

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A novel lectin was isolated from Bothrops leucurus snake venom using a combination of affinity and gel filtration chromatographies. The lectin (BlL) agglutinated glutaraldehyde-treated rabbit and human erythrocytes with preference for rabbit erythrocytes. Galactose, raffinose, lactose, fetal bovine serum and casein inhibited lectin-induced rabbit erythrocyte agglutination. BlL, with a molecular mass of 30 kDa and composed of two subunits of 15 kDa, showed dependence on calcium. BlL is an acidic protein with highest activity over the pH range of 4.0-7.0 and stable under heating to 70°C. Fluorescence emission spectra showed tryptophan residues partially buried within the lectin structure. The percentages of secondary structure revealed by circular dichroism were 1% α-helix, 44% β-sheet, 24% β-turn and 31% unordered. BlL showed effective antibacterial activity against Gram-positive bacteria Staphylococcus aureus, Enterococcus faecalis and Bacillus subtilis with minimal inhibitory concentrations of 31.25, 62.25 and 125 μg/mL, respectively. In conclusion, B. leucurus snake venom contains a galactoside-binding lectin with antibacterial activity.

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Isolation of a galactose-binding lectin from the venom of the snake Bothrops godmani (Godmann's pit viper)

Cited by 32 publications

References 9 publications

Coralsnake Venomics: Analyses of Venom Gland Transcriptomes and Proteomes of Six Brazilian Taxa

Coralsnake Venomics: Analyses of Venom Gland Transcriptomes and Proteomes of Six Brazilian Taxa

Quantitative high-throughput profiling of snake venom gland transcriptomes and proteomes (Ovophis okinavensis and Protobothrops flavoviridis)

Purification of a lectin with antibacterial activity from Bothrops leucurus snake venom

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