Isolation of a highly purified myelin protein

A fluorine NMR study of model membranes containing 19F‐labeled phospholipids and an intrinsic membrane protein

“…Myelin was obtained from bovine brain white matter as in [ 131. Lipophilin was prepared by the method in [2] and stored in the lyophilized form. SDS gel electrophoresis showed a single band of 32 000 Mr.…”

“…This protein has no known enzymatic function but is a so-called structural protein [l]. Its precise function is not known, but it is supposed to be mainly embedded in the bilayer, because this protein is characterized by its large content of apolar amino acid residues and because it has the rather uncommon property for proteins of being soluble in organic solvents [2].…”

Association of proteolipid apoproteins from bovine myelin with phospholipid in bilayer vesicles

“…The chloroform-methanol extract containing the proteolipid proteins was delipidated by gel fdtration on a Sephadex LH-20 (Pharmacia Ltd) column (2.5 X 70 cm) in chloroform/methanol/0.01 M HC1 (50:50:1, by vol.) [9]. This procedure was repeated to ensure complete delipidation of the proteolipid proteins as determined by thin-layer chromatography and phosphorus analysis [10].…”

In vitro Inhibition of Negative Strand Virus Transcriptase Activity by Proteins Soluble in Acidic Chloroform-Methanol

“…The M protein isolated from the virions of negative strand viruses possesses some physical and chemical peculiarities; one of the most important of them is its solubility in chloroformmethanol mixture at acid pH (acidic chloroform-methanol, ACM) (Gregoriades, 1973). Proteins soluble in ACM (ACMS proteins) have been isolated not only from viruses, but from membrane structures of myelin, mitochondria and bacteria as well (Folch & Less, 1951 ;Gagnon et al, 1971 ;Sanderman & Strominger, 1971 ;Tzagaloff & Kawai, 1972).…”