Isolation of a <i>Coxiella burnetii</i> Strain That Has Low Virulence for Mice from a Patient with Acute Q Fever

Oda, Hiroshi; Yoshiie, Kiyotaka

doi:10.1111/j.1348-0421.1989.tb00984.x

Cited by 27 publications

(22 citation statements)

References 9 publications

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“…The nested PCR assays were then performed to determine the detection limit. C. burnetii viability test: When C. burnetii DNA was detected from a sample by nested PCR, the viability of C. burnetii was assessed by inoculating the samples into mice [21]. The refrigerated sample was treated according to the method stated above, except that the first incubation temperature was 50°C.…”

Section: Sequencing Of Fragments Of 3 Target Genesmentioning

confidence: 99%

Development of a Method for Detecting Coxiella burnetii in Cheese Samples

et al. 2012

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ABSTRACT. Coxiella burnetii is the causative agent of Q fever, and the main route of infection in humans is inhalation of contaminated aerosols. Although oral transmission by contaminated raw milk or dairy products is also a possible route of human infection, there have been few studies investigating the presence of C. burnetii in dairy products. We developed a new method of extracting DNA from cheese and detecting C. burnetii DNA in cheese samples with a nested PCR assay. The limit of detection was 6.0  10 2 C. burnetii particles per gram. We subsequently used this method to examine the presence of C. burnetii in cheese at commercial markets in Tokyo from June 2005 to December 2008. Twenty-eight of 147 cheese samples were found to be positive for C. burnetii DNA. However, when we assessed the viability of C. burnetii by inoculating mice with DNA-positive samples, all of the samples were found to be negative. Thus, the viability of C. burnetii appears to have been lost in these cheese samples.

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Section: Sequencing Of Fragments Of 3 Target Genesmentioning

confidence: 99%

Development of a Method for Detecting Coxiella burnetii in Cheese Samples

et al. 2012

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“…In addition, several reports of isolation of C. burnetii from humans, cattle and ticks have been published [14,15,18,19]. However, there are no records of the infection among dairy cattle with reproductive disorders.…”

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Prevalence of Coxiella burnetii Infection in Dairy Cattle with Reproductive Disorders.

Htwe

Kako

et al. 1998

The Journal of Veterinary Medical Science

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ABSTRACT. The prevalence of Coxiella burnetii infection in 207 cattle with reproductive disorders was studied by using an indirect immunofluorescence (IF) test, nested polymerase chain reaction (PCR) and isolation. IF antibodies to phase I and phase II antigens of C. burnetii were found in 122 (58.9%) and 125 (60.4%) of the sera, respectively, and PCR-positives were found in 8 (3.9%) of the sera and in 51 (24.6%) of the milk samples. In addition, C. burnetii was isolated from 51 (24.6%) of the milk samples by inoculating laboratory mice. The results indicate that the IF test plus PCR are useful in the diagnosis of bovine coxiellosis. It is difficult to deny that dairy cattle with reproductive disorders would be one of the important reservoirs of C. burnetii responsible for infection in both animal and human populations in Japan. -KEY WORDS: bovine coxiellosis, Coxiella burnetii, nested polymerase chain reaction.

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“…of isolation of C. burnetii from an acute Q fever patient who became ill shortly after returning to Japan from Canada in 1989 [51].…”

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confidence: 99%