Isolation of a Marine-derived Flammeovirga sp. mbrc-1 Strain and Characterization of Its Agarase

장혜지,; 이동근,; 이승우,; 전명제,; 천원주,; 권개경,; Lee), 이희순(Hee Soon; 이상현,

doi:10.7841/ksbbj.2011.26.6.552

Cited by 5 publications

(3 citation statements)

References 19 publications

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“…Agaropectin과 agarose로 구성되어 있는 다당류인 한천 (agar)은 홍조류의 세포벽성분으로 3,6-anhydro α-L-gal-actopyranose와 β-D-galactose의 중합체이다 [3,19] [17,20]. 따라서 많은 연구진 들이 한천분해효소를 생산하는 균주를 찾기 위해 많은 노력을 기울이고 있어 Agarivorans 속 [10,16], Bacillus 속 [22], Cellvibrio 속 [1], Flammeovirga 속 [4], Glaciecola 속 [12,17], Microbulbifer 속 [8], Pseudoalteromonas 속 [18,23], Saccharophagus 속 [14], Sphingomonas 속 [6], Thalassomonas 속 [11]…”

Section: 서 론unclassified

Characterization of β-agarase from Isolated Simiduia sp. SH-4

Kim¹,

Lee²,

Jo³

et al. 2016

Journal of Life Science

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Agarases are classified into α-agarase and β-agarase that produce agarooligosaccharides and neoagarooligosaccharides, respectively. Neoagarooligosaccharides have whitening effect of skin, delay of starch degradation, and inhibition of bacterial growth etc. Hence, the object of this study was to isolate a novel agarase producing marine bacterium and characterization of its β-agarase. A novel agar-degrading bacterium was isolated from seashore of Namhae at Gyeongnamprovine, Korea and purely cultured with Marine agar 2216 media. The isolated bacterium was identified as Simiduia sp. SH-4 after 16S rRNA gene sequencing. The enzymatic sample was obtained from culture media of Simiduia sp. SH-4. Enzymatic activity was highly increased from 20(30% relative activity) to 30℃ (100%) and decreased from 30 to 40℃(75%) and so more. Relative activity was 100% at pH 6 while those were about 91% and 59% at pH 5.0 and 7.0, respectively, meaning the enzyme possesses narrow optimal pH range. Hence, the enzyme exhibited the maximal activity with 120.4 units/l at pH 6.0 and 30℃ in 20 mM Tris-HCl buffer. Thin layer chromatography (TLC) analysis showed that Simiduia sp. SH-4 produces β-agarase, which hydrolyze agarose to produce biofunctional neoagarooligosaccharides such as neoagarotetraose and neoagarobiose. Hence, broad applications would be possible using Simiduia sp. SH-4 and its enzyme in the food industry, cosmetics and medical fields.

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Section: 서 론unclassified

Characterization of β-agarase from Isolated Simiduia sp. SH-4

Kim¹,

Lee²,

Jo³

et al. 2016

Journal of Life Science

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“…한천 (agar)은 galactose와 galactopyranose의 중합체로, 홍조 류의 세포벽 구성성분인 점질성의 난소화성 복합다당류이 다 [1,2]. 한천은 3,6-anhydro-α-L-galactopyranosyl-(1,3)-D-galactopyranose가 β-1,4 결합으로 연결된 heteropolymer 형태의 D, L-galactan인 agarose (70%)와 sulfate esters, pyruvate acetal과 methyl ethers 등에 의해 수식된 3,6-anhydro-α-L-galactose가 존재하는 agaropectin (30%)으로 구성되어 있다 [3][4][5].…”

Section: Introductionunclassified

Characterization of α-agarase from Alteromonas sp. SH-1

Lee¹,

Shin²,

Kim³

et al. 2016

KSBB Journal

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A novel agar-degrading marine bacterium, SH-1 strain, was isolated from seashore of Namhae at Gyeongnam province, Korea. The SH-1 strain exhibited 98% similarity with Alteromonas species based on 16S rDNA sequencing and named as Alteromonas sp. SH-1. Alteromonas sp. SH-1 showed agarase activity of 348.3 U/L (1.67 U/mg protein). The molecular masses of the enzymes were predicted as about 85 kDa and 110 kDa by SDS-PAGE and zymogram. The enzymatic activity was optimal at 30 o C and the relative agarase activity was decreased as temperature increase from 30 o C and thus about 90% and 70% activities were shown at 40 o C and 50 o C, respectively. The optimum pH was 6.0 for agarase activity in 20 mM Tris-HCl buffer and activities were less than 70% and 85% activity at pH 5.0 and pH 7.0, respectively, compared with that at pH 6. Agarase activity has remained over 90% at 20 o C after 1.5 hour exposure at this temperature. However, its activity was less than 60% at 30 o C after 0.5 h exposure at this temperature. The enzymes produced agarooligosaccharides such as agaropentaose and agarotriose from agarose, indicating that the agarases are α-agarases. Thus, Alteromonas sp. SH-1 and its agarases would be useful for the industrial production of agarooligosaccharides which are known as having anticancer and antioxidation activities.

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“…따라서 고기능성인 한천분해산물의 생산을 위해 한천분해 효소를 생산하는 균주에 대한 연구들이 많이 이루어지고 있 으며 특히 β-agarase에 관한 연구가 최근까지 많이 보고되 고 있다. 지금까지 보고된 균주로는 Cellvibrio 속 [3], Alteromonas 속 [4], Microbulbifer 속 [11], Pseudoalteromonas 속 [14,20], Flammeovirga 속 [8], Glaciecola 속 [17], Thalassomonas 속 [16], Neiella 속 [18]…”

unclassified

Isolation of a New Agar Degrading Bacterium, Maribacter sp. SH-1 and Characterization of its Agarase

Lee¹,

Lee²,

Lee³

et al. 2016

Microbiology and Biotechnology Letters

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In this study, we isolated a new agar-degrading marine bacterium and characterized its agarase. An agardegrading marine bacterium SH-1 was isolated from seawater, collected from the seashore of Namhae in Gyeongnam province, Korea, and cultured in marine agar 2216 media. It was identified as Maribacter. sp. SH-1 by phylogenetic analyses, based on 16S rRNA gene sequence. The extracellular agarase was extracted from culture media of Maribacter sp. SH-1 and characterized.

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Isolation of a Marine-derived Flammeovirga sp. mbrc-1 Strain and Characterization of Its Agarase

Cited by 5 publications

References 19 publications

Characterization of β-agarase from Isolated Simiduia sp. SH-4

Characterization of β-agarase from Isolated Simiduia sp. SH-4

Characterization of α-agarase from Alteromonas sp. SH-1

Isolation of a New Agar Degrading Bacterium, Maribacter sp. SH-1 and Characterization of its Agarase

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