Isolation of a New <i>Orientia tsutsugamushi</i> Serotype

Seong, Seung Yong; Park, Sae Gwang; Kim, Hang Rae; Han, Tae Hee; Kang, Jae Seung; Choi, Man-Sik; Kim, Ik Sang; Chang, Woo Hyun

doi:10.1111/j.1348-0421.1997.tb01876.x

Cited by 10 publications

(7 citation statements)

References 23 publications

(22 reference statements)

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“…However, we failed to obtain a Yonchon-specific fragment. The realignment of the nucleotide sequence made by Seong and others 32 resulted in a dramatic increase of the sequence similarities among those of the 7 serotypes of O. tsutsugamushi. This implies that the hypervariability was due in part to alignment error and likely explains why a Yonchon-specific band was not detected by PCR.…”

Section: Resultsmentioning

confidence: 99%

Determination and geographical distribution of Orientia tsutsugamushi serotypes in Korea by nested polymerase chain reaction.

Ree¹,

Kim²,

Lee³

et al. 2001

The American Journal of Tropical Medicine and Hygiene

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Abstract. Field rodents and chigger mites were collected at 30 locations in Korea in October and November 1997-1999 to determine the serotypes of Orientia tsutsugamushi and their geographical distribution. A nested polymerase chain reaction was performed with the spleen tissues from 546 field-striped mice (Apodemus agrarius) and 104 pools of chigger mites. The positivity rate of O. tsutsugamushi was 45.6% in A. agrarius and 39.4% in the chigger mite pools. Two serotypes, Boryong and Karp, were found in these samples; the former was predominant (78.3% in the mice and 82.9% in the chigger mite pools), with wide distribution throughout the country, including Cheju-do. The latter was confined to the middle of the Korean peninsula, with positivity rates of 15.7% in the mice and 12.2% in the chigger mite pools. The double infection of Karp and Boryong serotypes was found in 15 (6.0%) A. agrarius mice. Gilliam serotype was not detected at any of the study locations. The Boryong and Kuroki serotypes were identical in amino acid sequence of the 56-kDa protein, although they differed in virulence to BALB/c mice.

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Section: Resultsmentioning

confidence: 99%

Determination and geographical distribution of Orientia tsutsugamushi serotypes in Korea by nested polymerase chain reaction.

Ree¹,

Kim²,

Lee³

et al. 2001

The American Journal of Tropical Medicine and Hygiene

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“…Serological antigen characterization, performed using monoclonal antibodies, of mouse-propagated Orientia isolates from humans identified the then-new type, the Boryong strain, as the most common (88 [78%] of 113 strains), followed by Karp (14%) and Gilliam (11%) [55,57,255]. Both monoclonal antibody serological characterization and phylogenetic tree analysis suggest that the Boryong strain is closely related to the Japanese Kuroki strain [19,137].…”

Section: Geographic Distribution Of Antigenic Typesmentioning

confidence: 99%

Scrub Typhus: The Geographic Distribution of Phenotypic and Genotypic Variants ofOrientia tsutsugamushi

et al. 2009

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Orientia tsutsugamushi is the etiological agent of scrub typhus, an acute, mite-borne, febrile illness that occurs in the Asia-Pacific region. Historically, strain characterization used serological analysis and revealed dramatic antigenic diversity. Eyeing a recommendation of potential vaccine candidates for broad protection, we review geographic diversity and serological and DNA prevalences. DNA analysis together with immunological analysis suggest that the prototype Karp strain and closely related strains are the most common throughout the region of endemicity. According to serological analysis, approximately 50% of isolates are seroreactive to Karp antisera, and approximately one-quarter of isolates are seroreactive to antisera against the prototype Gilliam strain. Molecular methods reveal greater diversity. By molecular methods, strains phylogenetically similar to Karp make up approximately 40% of all genotyped isolates, followed by the JG genotype group (Japan strains serotypically similar to the Gilliam strain but genetically non-Gilliam; 18% of all genotyped isolates). Three other genotype groups (Kato-related, Kawasaki-like, and TA763-like) each represent approximately 10% of genotyped isolates. Strains genetically similar to the Gilliam strain make up only 5% of isolates. Strains from these groups should be included in any potential vaccine.

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“…Also, variant serotypes have been reported in China, Japan, Thailand, and other Asian countries (Enatsu et al, 1999;Qiang et al, 2003;Blacksell et al, 2008). Some other strains such as the Yongworl, Pajoo, and Yonchon are identified as causative agents of O. tsutsugamushi in Korea (Chang et al, 1990;Seong et al, 1997;Shim et al, 2005;Yamashita et al, 1988). 56 kDa protein antigen is a primary antigen found in the sera of humans infected with scrub typhus.…”

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confidence: 95%

Phylogenetic clustering of 4 prevalent virulence genes in Orientia tsutsugamushi isolates from human patients

et al. 2010

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The pathogenicity of microbes is involved in many kinds of virulence genes. The relationships between these virulence genes and strains are not clear in Orientia tsutsugamushi yet. In this study, we confirmed the presence of the virulence genes and classified into O. tsutsugamushi isolates using phylogenetic analysis of the virulence genes. We also compared the fatality rates of every isolate via an infection experiment in BALB/c mice using the O. tsutsugamushi isolates, Deajeon03-01, Wonju03-01, and Muju03-01. Moreover, we compared the phylogenetic analysis, in basis with 56 kDa protein sequence which determined from serotype, and virulence genes of O. tsutsugamushi. Our results showed remarkably different fatality rates between Deajeon03-01 and Muju03-01, which are both Boryong strains of O. tsutsugamushi. Also, clustering analyses including these two isolates gave slightly different results depending on whether they were clustered based on virulence genes or on the 56 kDa protein sequences. Consequently, we conclude that fatality rates in O. tsutsugamushi are correlated with differences in both serotypes and virulence genes. We identified some variations within the virulence genes dnaA, virB8, tolR, and trxA among the isolates.

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Isolation of a New Orientia tsutsugamushi Serotype

Cited by 10 publications

References 23 publications

Determination and geographical distribution of Orientia tsutsugamushi serotypes in Korea by nested polymerase chain reaction.

Determination and geographical distribution of Orientia tsutsugamushi serotypes in Korea by nested polymerase chain reaction.

Scrub Typhus: The Geographic Distribution of Phenotypic and Genotypic Variants ofOrientia tsutsugamushi

Phylogenetic clustering of 4 prevalent virulence genes in Orientia tsutsugamushi isolates from human patients

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