Isolation of a novel alginate lyase‐producing <i>Bacillus litoralis</i> strain and its potential to ferment <i>Sargassum horneri</i> for biofertilizer

Wang, Mingpeng; Chen, Lei; Liu, Zhengyi; Zhang, Zhaojie; Qin, Song; Yan, Peisheng

doi:10.1002/mbo3.387

Cited by 26 publications

(17 citation statements)

References 48 publications

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“…As a biofertilizer, seaweed is a sustainable alternative to chemical fertilizers. Furthermore, advanced production technology enhances the nutrient content of seaweed extract (Kadam et al, 2015;Wang et al, 2016a). Previous studies have reported the beneficial effects of seaweed extract application in the agriculture domain, such as early seed germination and seedling establishment, improved crop yield and quality, increased resistance to environmental stresses, and regulation of the soil micro-ecosystem (Bai et al, 2013;El Kaoaua et al, 2013;Mohamed and El-Sehrawy, 2013).…”

Section: Introductionmentioning

confidence: 99%

Responses of soil microbial communities to a short-term application of seaweed fertilizer revealed by deep amplicon sequencing

Wang

Chen

et al. 2018

Applied Soil Ecology

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Numerous studies have reported soil damage from chemical fertilizer application and an obvious promotional effect of seaweed fertilizer fermented with Sargassum horneri on the growth of tomato roots and seedlings due to its alginate oligosaccharide. However, few studies have assessed the effects of the fermented seaweed fertilizer on ecological environment and microorganisms in soil. Herein, our objective is to uncover microbial and soil environmental responses to Sargassum horneri-fermented seaweed fertilizer. After treated tomato-planting plots with Sargassum horneri fermented seaweed fertilizer, soil bacterial community compositions based on 16S rRNA gene amplicon sequencing, enzyme activities in soil and crop yield were analyzed. The bacterial α-diversity was strongly influenced by seaweed fertilizer amendment after 60 days. Non-metric multidimensional scaling (NMDS) analysis showed that a difference in bacterial community compositions between day 0 and day 60 was obvious for soil treated with seaweed fertilizer. The community variation could be caused by invertase activity and dehydrogenase activity in canonical correlation analysis (CCA). Protease activity, polyphenol oxidase activity and urease activity showed an obvious correlation with community variation in the Mantel test. The fertilization increased tomato yield by 1.48-1.83 times, Vc content by 1.24-4.55 times and lycopene content by 1.20-2.33 times. In the present study, a possible reason for bacterial community variation was discovered, which will provide an economical dilution rate of seaweed fertilizer for optimal crop yield and quality. Meanwhile, our study will be beneficial for developing a possible substitute for chemical fertilizer and an improved understanding of soil microbial functions and soil sustainability.

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Section: Introductionmentioning

confidence: 99%

Responses of soil microbial communities to a short-term application of seaweed fertilizer revealed by deep amplicon sequencing

Wang

Chen

et al. 2018

Applied Soil Ecology

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“…We used TLC to check the oligosaccharide profiles [ 37 ] of commercial SA and aqueous Wakame extract ( Fig 1a ). Similar TLC profiles confirm the similarities of oligosaccharide compositions of SA and Wakame extract.…”

Section: Resultsmentioning

confidence: 99%

“…TLC plates were developed using a mixture of 1-butanol, formic acid, and water in 4:6:1 (v:v:v) ratio. The developed TLC plates were heated at 110°C for 5 min after spraying with 10% (v/v) sulfuric acid in ethanol to test for the presence of alginate oligosaccharides [ 37 ].…”

Section: Methodsmentioning

confidence: 99%

Antimicrobial biopolymer formation from sodium alginate and algae extract using aminoglycosides

et al. 2019

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Antimicrobial biopolymers provide a biodegradable, sustainable, safe, and cheap approach to drug delivery and wound dressing to control bacterial infection and improve wound healing respectively. Here, we report a one-step method of making antimicrobial alginate polymer from sodium alginate and aqueous extract of Wakame using antibiotic aminoglycosides. Thin layer chromatography of commercially available sodium alginate and Wakame extract showed similar oligosaccharide profiles. Screening of six aminoglycosides showed that kanamycin disulfate and neomycin sulfate produces the highest amount of biopolymer; however, kanamycin disulfate produces the most malleable and form fitting biopolymer. Image texture analysis of biopolymers showed similar quantification parameters for all the six aminoglycosides. Weight of alginate polymer as a function of aminoglycoside concentration follows a growth model of prion protein, consistent with the aggregating nature of both processes. Slow release of antibiotics and the resulting zone of inhibition against E. coli DH5α were observed by agar well diffusion assay. Inexpensive method of production and slow release of antibiotics will enable diverse applications of antimicrobial alginate biopolymer reported in this paper.

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“…The enzymes secreted by these Bacteroidetes hydrolyze alginate (Li, Shang, Li, Wang, & Yu, ). Alginate‐degrading bacteria, such as Gracilibacillus A7 (Tang, Taniguchi, Chu, Zhou, & Nagata, ), Bacillus subtilis KCTC 11782BP (Bang et al., ; Yang et al., ), Bacillus litoralis strain M3 (Wang, Chen et al., ), Flavobacterium sp. strain LXA (An et al., ), and Pseudoalteromonas agarovorans CHO‐12 (Choi, Piao, Shin, & Cho, ), have been isolated and utilized for AOS production.…”

Section: Production Of Aosmentioning

confidence: 99%

Alginate Oligosaccharides: Production, Biological Activities, and Potential Applications

Liu

Yang

et al. 2019

Comp Rev Food Sci Food Safe

227

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Alginate, a group of polyuronic saccharides, has been widely used in both pharmaceutical and food industries due to its unique physicochemical properties as well as beneficial health effects. However, the potential applications of alginate are restricted because of its low water solubility and high solution viscosity when significant concentrations are needed, particularly in food products. Alginate oligosaccharides (AOS), oligomers containing 2 to 25 monomers, can be obtained via hydrolysis of glycosidic bonds, organic synthesis, or through biosynthesis. Generally, AOS have shorter chain lengths and thus improved water solubility when compared with higher molecular weight alginates of the same monomers. These oligosaccharides have attracted interest from both basic and applied researchers. AOS have unique bioactivity and can impart health benefits. They have shown immunomodulatory, antimicrobial, antioxidant, prebiotic, antihypertensive, antidiabetic, antitumor, anticoagulant, and other activities. As examples, they have been utilized as prebiotics, feed supplements for aquaculture, poultry, and swine, elicitors for plants and microorganisms, cryoprotectors for frozen foods, and postharvest treatments. This review comprehensively covers methods for AOS production from alginate, such as physical/chemical methods, enzymatic methods, fermentation, organic synthesis, and biosynthesis. Moreover, current progress in structural characterization, potential health benefits, and AOS metabolism after ingestion are summarized in this review. This review will discuss methods for producing and modified AOS with desirable structures that are suited for novel applications.

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Isolation of a novel alginate lyase‐producing Bacillus litoralis strain and its potential to ferment Sargassum horneri for biofertilizer

Cited by 26 publications

References 48 publications

Responses of soil microbial communities to a short-term application of seaweed fertilizer revealed by deep amplicon sequencing

Responses of soil microbial communities to a short-term application of seaweed fertilizer revealed by deep amplicon sequencing

Antimicrobial biopolymer formation from sodium alginate and algae extract using aminoglycosides

Alginate Oligosaccharides: Production, Biological Activities, and Potential Applications

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