Isolation of A Novel Bacterial Strain Planococcus Plakortidi from Ross Broiler Chicken Gut (CECUM) with Combination of Non-Starch Polysaccride Degrading Pectinase Enzyme

Abstract

Purpose: In many countries, including Pakistan, broiler chickens and the poultry industry are severely affected by bacteria. Broiler chickens are infected with Gram-positive and Gram-negative bacteria. Methods & Findings: The current study successfully isolated non-starch polysaccharide (NSP) degrading a novel strain Planococcusplakortidi (PCB2) from the chicken gut cecum, screened out from 60 samples. Practical Implication & Conclusion: Furthermore, the isolate was taxonomically characterized based on its cellular morphology, physiological, and chemotaxonomic characteristics, as well as the phylogenetic similarity index of the 16S rRNA gene sequence. The bacterium was found to exhibit maximum production of pectinase enzyme on the eighth day of incubation at 37℃. Keywords: Broiler chicken, gut microbiota, cecal bacterial diversity, chicken food digestion, Non-starch Polysaccharides

Impacts of Solid-State Fermented Barley with Fibrolytic Exogenous Enzymes on Feed Utilization, and Antioxidant Status of Broiler Chickens

“…Other possible explanations for the enhanced broiler performance are that fermented barley-based diets treated with exogenous NSPs-degrading enzymes decrease the digesta viscosity, prompt the degradation of the cell wall, release the encapsulated nutrients, and induce gut microbiota modification via prebiotic oligosaccharides [71][72][73]. The viscous contents inside the intestines of birds fed with barley-based diets unlike birds fed with corn-based diets were noted due to their higher β-glucans content [74].…”